Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891277524> ?p ?o ?g. }
- W2891277524 endingPage "2402" @default.
- W2891277524 startingPage "2391" @default.
- W2891277524 abstract "α-Amyrin is a plant-derived pentacyclic triterpenoid, with a lot of important physiological and pharmacological activities. The formation of α-amyrin from (3 S)-2,3-oxidosqualene is catalyzed by α-amyrin synthase (α-AS), a member of the oxidosqualene cyclase (OSC) protein family. However, α-amyrin is not yet commercially developed due to its extremely low productivity in plants. The engineered Saccharomyces cerevisiae with efficient α-amyrin production pathway could be used as an alternative and sustainable solution to produce α-amyrin from renewable raw materials. To efficiently improve α-amyrin production in S. cerevisiae, we identified two α-ASs, EjAS and MdOSC1 from Eriobotrya japonica and Malus × domestica, respectively, through strict bioinformatics screening criteria and phylogenetic analysis. The specific activities of purified EjAS and MdOSC1 were 0.0032 and 0.0293 μmol/min/mg, respectively. EjAS produced α-amyrin and β-amyrin at a ratio of 17:3, MdOSC1 produced α-amyrin, β-amyrin and lupeol at a ratio of 86:13:1, indicating MdOSC1 had significantly higher specific activity and higher ratio of α-amyrin than EjAS. Furthermore, MdOSC1 was introduced into S. cerevisiae combining with the increased supply of (3 S)-2,3-oxidosqualene to achieve the encouraging α-amyrin production, and the titer of α-amyrin achieved 11.97 ± 0.61 mg/L, 5.8 folds of the maximum production reported." @default.
- W2891277524 created "2018-09-27" @default.
- W2891277524 creator A5012318541 @default.
- W2891277524 creator A5018863416 @default.
- W2891277524 creator A5034711601 @default.
- W2891277524 creator A5040622965 @default.
- W2891277524 creator A5053158139 @default.
- W2891277524 creator A5057584601 @default.
- W2891277524 creator A5063070943 @default.
- W2891277524 creator A5072246677 @default.
- W2891277524 creator A5088030846 @default.
- W2891277524 creator A5089817900 @default.
- W2891277524 date "2018-09-14" @default.
- W2891277524 modified "2023-10-16" @default.
- W2891277524 title "Productive Amyrin Synthases for Efficient α-Amyrin Synthesis in Engineered <i>Saccharomyces cerevisiae</i>" @default.
- W2891277524 cites W1498901232 @default.
- W2891277524 cites W1567072612 @default.
- W2891277524 cites W1596041927 @default.
- W2891277524 cites W1967108492 @default.
- W2891277524 cites W1968212788 @default.
- W2891277524 cites W1986482685 @default.
- W2891277524 cites W1994433688 @default.
- W2891277524 cites W2009414057 @default.
- W2891277524 cites W2019065272 @default.
- W2891277524 cites W2022657578 @default.
- W2891277524 cites W2026688176 @default.
- W2891277524 cites W2026899324 @default.
- W2891277524 cites W2036032792 @default.
- W2891277524 cites W2049773266 @default.
- W2891277524 cites W2053689785 @default.
- W2891277524 cites W2058102274 @default.
- W2891277524 cites W2063554961 @default.
- W2891277524 cites W2070470282 @default.
- W2891277524 cites W2074126983 @default.
- W2891277524 cites W2075602575 @default.
- W2891277524 cites W2093905539 @default.
- W2891277524 cites W2110952175 @default.
- W2891277524 cites W2124478635 @default.
- W2891277524 cites W2124838044 @default.
- W2891277524 cites W2142766362 @default.
- W2891277524 cites W2148656018 @default.
- W2891277524 cites W2149792885 @default.
- W2891277524 cites W2155010685 @default.
- W2891277524 cites W2164098358 @default.
- W2891277524 cites W2166813071 @default.
- W2891277524 cites W2335572167 @default.
- W2891277524 cites W2408221009 @default.
- W2891277524 cites W2460043299 @default.
- W2891277524 cites W2580848968 @default.
- W2891277524 cites W2588403571 @default.
- W2891277524 cites W2591200718 @default.
- W2891277524 cites W2602443505 @default.
- W2891277524 cites W2610080414 @default.
- W2891277524 cites W2612303549 @default.
- W2891277524 cites W2619195667 @default.
- W2891277524 cites W2733166765 @default.
- W2891277524 cites W2748956475 @default.
- W2891277524 cites W2784492203 @default.
- W2891277524 cites W3193177704 @default.
- W2891277524 cites W50360755 @default.
- W2891277524 doi "https://doi.org/10.1021/acssynbio.8b00176" @default.
- W2891277524 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30216049" @default.
- W2891277524 hasPublicationYear "2018" @default.
- W2891277524 type Work @default.
- W2891277524 sameAs 2891277524 @default.
- W2891277524 citedByCount "30" @default.
- W2891277524 countsByYear W28912775242019 @default.
- W2891277524 countsByYear W28912775242020 @default.
- W2891277524 countsByYear W28912775242021 @default.
- W2891277524 countsByYear W28912775242022 @default.
- W2891277524 countsByYear W28912775242023 @default.
- W2891277524 crossrefType "journal-article" @default.
- W2891277524 hasAuthorship W2891277524A5012318541 @default.
- W2891277524 hasAuthorship W2891277524A5018863416 @default.
- W2891277524 hasAuthorship W2891277524A5034711601 @default.
- W2891277524 hasAuthorship W2891277524A5040622965 @default.
- W2891277524 hasAuthorship W2891277524A5053158139 @default.
- W2891277524 hasAuthorship W2891277524A5057584601 @default.
- W2891277524 hasAuthorship W2891277524A5063070943 @default.
- W2891277524 hasAuthorship W2891277524A5072246677 @default.
- W2891277524 hasAuthorship W2891277524A5088030846 @default.
- W2891277524 hasAuthorship W2891277524A5089817900 @default.
- W2891277524 hasConcept C185592680 @default.
- W2891277524 hasConcept C2777576037 @default.
- W2891277524 hasConcept C2779222958 @default.
- W2891277524 hasConcept C55493867 @default.
- W2891277524 hasConceptScore W2891277524C185592680 @default.
- W2891277524 hasConceptScore W2891277524C2777576037 @default.
- W2891277524 hasConceptScore W2891277524C2779222958 @default.
- W2891277524 hasConceptScore W2891277524C55493867 @default.
- W2891277524 hasFunder F4320321001 @default.
- W2891277524 hasIssue "10" @default.
- W2891277524 hasLocation W28912775241 @default.
- W2891277524 hasLocation W28912775242 @default.
- W2891277524 hasOpenAccess W2891277524 @default.
- W2891277524 hasPrimaryLocation W28912775241 @default.
- W2891277524 hasRelatedWork W1566492806 @default.
- W2891277524 hasRelatedWork W1985480577 @default.