FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891284543> ?p ?o ?g. }

• W2891284543 endingPage "376" @default.
• W2891284543 startingPage "376" @default.
• W2891284543 abstract "Strawberry (Fragaria × ananassa Duch.) is an important crop in Spain. In January 2018, ‘Florida Fortuna’ strawberry plants exhibiting poor growth were observed in a fruit production field in Palos de la Frontera, Huelva (southwestern Spain). These plants were from nurseries in Avila Province (Spain) that had soils with a high clay content. Affected plants showed varying degrees of stunting, black necrosis on basal petioles, brown to dark-brown discoloration in roots, and few new roots emerging from the crown. Two foci were found and surveyed (six plants per focus). Petiole, crown, and root pieces of symptomatic plants were surface sterilized in 1% sodium hypochlorite for 2 min, rinsed in sterile distilled water, and air-dried in a laminar flow cabinet. Disinfested pieces were transferred to Petri dishes containing potato dextrose agar (PDA) and incubated for 7 days at 25°C. Five fungal isolates were recovered. Colonies were white to pale brown and had floccose aerial mycelia. Two isolates were purified by cutting hyphal tips from their margins. Microscopic examination showed morphological characters coinciding with Rhizoctonia (Sneh et al. 1991). Nuclear staining with acridine orange solution (20 μl/ml), prepared in acetic acid buffer, pH 7.2, for 15 min (Seema et al. 2012) showed two nuclei per cell in the hyphae of the two isolates. Mean hyphal diameter was 4.79 µm. No sclerotia were formed after 20 days on PDA at 25°C. The morphological characteristics of the isolates resembled those of binucleate Rhizoctonia (BNR). Species identity was confirmed for one isolate by sequencing the intergenic spacer (ITS) region of ribosomal DNA with the primers ITS1 and ITS4 (White et al. 1990). Identities of the resulting sequences were confirmed by BLAST analysis. The sequences were 100% identical to those of Ceratobasidium sp. AG-A KC782943 and were deposited as MH666073 in GenBank. Hyphal anastomosis tests were conducted by paring isolates with seven known tester isolates of BNR AG on 2% water agar. Anastomosis was observed with AG-A tester isolate. The isolates were identified as a BNR AG-A. To confirm the pathogenicity of the isolate, five runner strawberry plants ‘Rociera FNM’ and five ‘Rabida FNM’ were inoculated with PDA colonized plugs 1 cm in diameter located at the crown base. Five noninoculated plants were used as negative controls. Plants were grown in 200-ml pots with peat in a growth chamber at 25/22°C (day/night) with a 12-h photoperiod. Symptoms started appearing on inoculated plants 18 days after inoculation. All inoculated plants showed the same symptoms described above. Disease severity was scored as proportion of affected or senescent leaves. Inoculated ‘Rociera FNM’ plants showed 36% more affected leaves than noninoculated ones, while this difference in ‘Rabida FNM’ plants was 29%. Inoculated ‘Rociera FNM’ plants also had significantly lower dry weights (mean value: 3.91 g) than control plants (mean value: 8.02 g). The fungus was reisolated and confirmed to be Rhizoctonia sp. The BNR AG-A is one of the most common groups associated with root rot and a serious threat to strawberry production worldwide (Fang et al. 2013). To our knowledge, this is the first report of BNR AG-A causing root rot of strawberry in Spain. From now, growers will have to consider this pathogen for soil health management and for diseased plant diagnosis." @default.
• W2891284543 created "2018-09-27" @default.
• W2891284543 creator A5019128613 @default.
• W2891284543 creator A5025838220 @default.
• W2891284543 creator A5063865158 @default.
• W2891284543 creator A5091751249 @default.
• W2891284543 date "2019-02-01" @default.
• W2891284543 modified "2023-10-17" @default.
• W2891284543 title "First Report of Root Rot on Strawberry Caused by Binucleate Rhizoctonia AG-K in Spain" @default.
• W2891284543 cites W1985129028 @default.
• W2891284543 cites W2120644786 @default.
• W2891284543 cites W2138489374 @default.
• W2891284543 doi "https://doi.org/10.1094/pdis-08-18-1301-pdn" @default.
• W2891284543 hasPublicationYear "2019" @default.
• W2891284543 type Work @default.
• W2891284543 sameAs 2891284543 @default.
• W2891284543 citedByCount "2" @default.
• W2891284543 countsByYear W28912845432020 @default.
• W2891284543 countsByYear W28912845432022 @default.
• W2891284543 crossrefType "journal-article" @default.
• W2891284543 hasAuthorship W2891284543A5019128613 @default.
• W2891284543 hasAuthorship W2891284543A5025838220 @default.
• W2891284543 hasAuthorship W2891284543A5063865158 @default.
• W2891284543 hasAuthorship W2891284543A5091751249 @default.
• W2891284543 hasBestOaLocation W28912845431 @default.
• W2891284543 hasConcept C104727253 @default.
• W2891284543 hasConcept C144027150 @default.
• W2891284543 hasConcept C2777040454 @default.
• W2891284543 hasConcept C2777919559 @default.
• W2891284543 hasConcept C2778394675 @default.
• W2891284543 hasConcept C2780104187 @default.
• W2891284543 hasConcept C2780476228 @default.
• W2891284543 hasConcept C59822182 @default.
• W2891284543 hasConcept C64502627 @default.
• W2891284543 hasConcept C86803240 @default.
• W2891284543 hasConcept C89423630 @default.
• W2891284543 hasConceptScore W2891284543C104727253 @default.
• W2891284543 hasConceptScore W2891284543C144027150 @default.
• W2891284543 hasConceptScore W2891284543C2777040454 @default.
• W2891284543 hasConceptScore W2891284543C2777919559 @default.
• W2891284543 hasConceptScore W2891284543C2778394675 @default.
• W2891284543 hasConceptScore W2891284543C2780104187 @default.
• W2891284543 hasConceptScore W2891284543C2780476228 @default.
• W2891284543 hasConceptScore W2891284543C59822182 @default.
• W2891284543 hasConceptScore W2891284543C64502627 @default.
• W2891284543 hasConceptScore W2891284543C86803240 @default.
• W2891284543 hasConceptScore W2891284543C89423630 @default.
• W2891284543 hasIssue "2" @default.
• W2891284543 hasLocation W28912845431 @default.
• W2891284543 hasOpenAccess W2891284543 @default.
• W2891284543 hasPrimaryLocation W28912845431 @default.
• W2891284543 hasRelatedWork W2043216660 @default.
• W2891284543 hasRelatedWork W2075214723 @default.
• W2891284543 hasRelatedWork W2096724802 @default.
• W2891284543 hasRelatedWork W2131451663 @default.
• W2891284543 hasRelatedWork W2142506840 @default.
• W2891284543 hasRelatedWork W2465118108 @default.
• W2891284543 hasRelatedWork W2891284543 @default.
• W2891284543 hasRelatedWork W3149042365 @default.
• W2891284543 hasRelatedWork W4321842782 @default.
• W2891284543 hasRelatedWork W54827795 @default.
• W2891284543 hasVolume "103" @default.
• W2891284543 isParatext "false" @default.
• W2891284543 isRetracted "false" @default.
• W2891284543 magId "2891284543" @default.
• W2891284543 workType "article" @default.