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- W2891290905 abstract "Gene regulatory networks used by Mycobacterium tuberculosis (Mtb) during infection include many genes of unknown function, confounding efforts to determine their roles in Mtb biology. Rv1265 encodes a conserved hypothetical protein that is expressed during infection and in response to elevated levels of cyclic AMP. Here, we report that Rv1265 is a novel auto-inhibitory ATP-binding transcription factor that upregulates expression of the small non-coding RNA Mcr11, and propose that Rv1265 be named ATP-binding mcr11 regulator (AbmR). AbmR directly and specifically bound DNA, as determined by electrophoretic mobility shift assays, and this DNA-binding activity was enhanced by AbmR's interaction with ATP. Genetic knockout of abmR in Mtb increased abmR promoter activity and eliminated growth phase-dependent increases in mcr11 expression during hypoxia. Mutagenesis identified arginine residues in the carboxy terminus that are critical for AbmR's DNA-binding activity and gene regulatory function. Limited similarity to other DNA- or ATP-binding domains suggests that AbmR belongs to a novel class of DNA- and ATP-binding proteins. AbmR was also found to form large organized structures in solution and facilitate the serum-dependent association of Mtb with human lung epithelial cells. These results indicate a potentially complex role for AbmR in Mtb biology." @default.
- W2891290905 created "2018-09-27" @default.
- W2891290905 creator A5001688784 @default.
- W2891290905 creator A5004307488 @default.
- W2891290905 creator A5022719681 @default.
- W2891290905 creator A5085966383 @default.
- W2891290905 creator A5091109601 @default.
- W2891290905 date "2018-10-21" @default.
- W2891290905 modified "2023-10-16" @default.
- W2891290905 title "AbmR (Rv1265) is a novel transcription factor of<i>Mycobacterium tuberculosis</i>that regulates host cell association and expression of the non-coding small RNA Mcr11" @default.
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