Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891300466> ?p ?o ?g. }
- W2891300466 endingPage "278" @default.
- W2891300466 startingPage "266" @default.
- W2891300466 abstract "γ-Amino butyric acid (GABA) mediated signaling is critical in the central and enteric nervous systems, pancreas, lungs, and other tissues. It is associated with many neurological disorders and craniofacial development. Glutamic acid decarboxylase (GAD) synthesizes GABA from glutamate, and knockdown of the gad1 gene results in craniofacial defects that are lethal in zebrafish. To bypass this and enable observation of the neurological defects resulting from knocking down gad1 expression, a photoactivatable morpholino oligonucleotide (MO) against gad1 was prepared by cyclization with a photocleavable linker rendering the MO inactive. The cyclized MO was stable in the dark and toward degradative enzymes and was completely linearized upon brief exposure to 405 nm light. In the course of investigating the function of the ccMOs in zebrafish, we discovered that zebrafish possess paralogous gad1 genes, gad1a and gad1b. A gad1b MO injected at the 1–4 cell stage caused severe morphological defects in head development, which could be bypassed, enabling the fish to develop normally, if the fish were injected with a photoactivatable, cyclized gad1b MO and grown in the dark. At 1 day post fertilization (dpf), light activation of the gad1b MO followed by observation at 3 and 7 dpf led to increased and abnormal electrophysiological brain activity compared to wild type animals. The photocleavable linker can be used to cyclize and inactivate any MO, and represents a general strategy to parse the function of developmentally important genes in a spatiotemporal manner." @default.
- W2891300466 created "2018-09-27" @default.
- W2891300466 creator A5014217825 @default.
- W2891300466 creator A5018729208 @default.
- W2891300466 creator A5019521201 @default.
- W2891300466 creator A5024564900 @default.
- W2891300466 creator A5026780383 @default.
- W2891300466 creator A5029873166 @default.
- W2891300466 creator A5068213512 @default.
- W2891300466 creator A5069049550 @default.
- W2891300466 creator A5075211129 @default.
- W2891300466 creator A5077554300 @default.
- W2891300466 creator A5086154216 @default.
- W2891300466 date "2018-09-10" @default.
- W2891300466 modified "2023-10-16" @default.
- W2891300466 title "Bypassing Glutamic Acid Decarboxylase 1 (Gad1) Induced Craniofacial Defects with a Photoactivatable Translation Blocker Morpholino" @default.
- W2891300466 cites W1516532373 @default.
- W2891300466 cites W1520420711 @default.
- W2891300466 cites W1552346480 @default.
- W2891300466 cites W1703858137 @default.
- W2891300466 cites W176212822 @default.
- W2891300466 cites W1875008998 @default.
- W2891300466 cites W1964948396 @default.
- W2891300466 cites W1966989580 @default.
- W2891300466 cites W1969400996 @default.
- W2891300466 cites W1974760604 @default.
- W2891300466 cites W1977164622 @default.
- W2891300466 cites W1983864650 @default.
- W2891300466 cites W1997222580 @default.
- W2891300466 cites W1998756807 @default.
- W2891300466 cites W2001555806 @default.
- W2891300466 cites W2003714509 @default.
- W2891300466 cites W2005439302 @default.
- W2891300466 cites W2007134228 @default.
- W2891300466 cites W2008377803 @default.
- W2891300466 cites W2009219349 @default.
- W2891300466 cites W2009382384 @default.
- W2891300466 cites W2009635392 @default.
- W2891300466 cites W2012166652 @default.
- W2891300466 cites W2017243089 @default.
- W2891300466 cites W2018059970 @default.
- W2891300466 cites W2019483700 @default.
- W2891300466 cites W2021744384 @default.
- W2891300466 cites W2022243850 @default.
- W2891300466 cites W2022906661 @default.
- W2891300466 cites W2023656585 @default.
- W2891300466 cites W2029559333 @default.
- W2891300466 cites W2030792858 @default.
- W2891300466 cites W2032573735 @default.
- W2891300466 cites W2032626095 @default.
- W2891300466 cites W2032742781 @default.
- W2891300466 cites W2032881196 @default.
- W2891300466 cites W2035043164 @default.
- W2891300466 cites W2035757731 @default.
- W2891300466 cites W2043601220 @default.
- W2891300466 cites W2047266773 @default.
- W2891300466 cites W2049106336 @default.
- W2891300466 cites W2052139633 @default.
- W2891300466 cites W2053336082 @default.
- W2891300466 cites W2056050981 @default.
- W2891300466 cites W2059956688 @default.
- W2891300466 cites W2060923069 @default.
- W2891300466 cites W2067011924 @default.
- W2891300466 cites W2067225454 @default.
- W2891300466 cites W2068411596 @default.
- W2891300466 cites W2068420002 @default.
- W2891300466 cites W2068718992 @default.
- W2891300466 cites W2070416972 @default.
- W2891300466 cites W2070572720 @default.
- W2891300466 cites W2071161962 @default.
- W2891300466 cites W2072652145 @default.
- W2891300466 cites W2075108037 @default.
- W2891300466 cites W2077727091 @default.
- W2891300466 cites W2078660236 @default.
- W2891300466 cites W2082628381 @default.
- W2891300466 cites W2086958758 @default.
- W2891300466 cites W2088550674 @default.
- W2891300466 cites W2092246706 @default.
- W2891300466 cites W2098205015 @default.
- W2891300466 cites W2099540110 @default.
- W2891300466 cites W2107176556 @default.
- W2891300466 cites W2112444339 @default.
- W2891300466 cites W2115473287 @default.
- W2891300466 cites W2117860217 @default.
- W2891300466 cites W2119222397 @default.
- W2891300466 cites W2122194908 @default.
- W2891300466 cites W2125349610 @default.
- W2891300466 cites W2126355146 @default.
- W2891300466 cites W2128284178 @default.
- W2891300466 cites W2128635872 @default.
- W2891300466 cites W2128719175 @default.
- W2891300466 cites W2132166460 @default.
- W2891300466 cites W2133178667 @default.
- W2891300466 cites W2143133343 @default.
- W2891300466 cites W2149154873 @default.
- W2891300466 cites W2149498611 @default.
- W2891300466 cites W2149868212 @default.
- W2891300466 cites W2150631626 @default.