FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891315832> ?p ?o ?g. }

• W2891315832 endingPage "10320" @default.
• W2891315832 startingPage "10315" @default.
• W2891315832 abstract "ssDNA, which is involved in numerous aspects of chromosome biology, is managed by a suite of proteins with tailored activities. The majority of these proteins bind ssDNA indiscriminately, exhibiting little apparent sequence preference. However, there are several notable exceptions, including the Saccharomyces cerevisiae Cdc13 protein, which is vital for yeast telomere maintenance. Cdc13 is one of the tightest known binders of ssDNA and is specific for G-rich telomeric sequences. To investigate how these two different biochemical features, affinity and specificity, contribute to function, we created an unbiased panel of alanine mutations across the Cdc13 DNA-binding interface, including several aromatic amino acids that play critical roles in binding activity. A subset of mutant proteins exhibited significant loss in affinity in vitro that, as expected, conferred a profound loss of viability in vivo. Unexpectedly, a second category of mutant proteins displayed an increase in specificity, manifested as an inability to accommodate changes in ssDNA sequence. Yeast strains with specificity-enhanced mutations displayed a gradient of viability in vivo that paralleled the loss in sequence tolerance in vitro, arguing that binding specificity can be fine-tuned to ensure optimal function. We propose that DNA binding by Cdc13 employs a highly cooperative interface whereby sequence diversity is accommodated through plastic binding modes. This suggests that sequence specificity is not a binary choice but rather is a continuum. Even in proteins that are thought to be specific nucleic acid binders, sequence tolerance through the utilization of multiple binding modes may be a broader phenomenon than previously appreciated." @default.
• W2891315832 created "2018-09-27" @default.
• W2891315832 creator A5017441144 @default.
• W2891315832 creator A5021223273 @default.
• W2891315832 creator A5029458441 @default.
• W2891315832 creator A5031343617 @default.
• W2891315832 creator A5043273992 @default.
• W2891315832 creator A5051761061 @default.
• W2891315832 creator A5066987254 @default.
• W2891315832 creator A5080730606 @default.
• W2891315832 date "2018-09-24" @default.
• W2891315832 modified "2023-09-26" @default.
• W2891315832 title "Single-stranded telomere-binding protein employs a dual rheostat for binding affinity and specificity that drives function" @default.
• W2891315832 cites W1948628402 @default.
• W2891315832 cites W1965580483 @default.
• W2891315832 cites W1973544851 @default.
• W2891315832 cites W1974988689 @default.
• W2891315832 cites W1975015488 @default.
• W2891315832 cites W1977780474 @default.
• W2891315832 cites W1979164620 @default.
• W2891315832 cites W1983634771 @default.
• W2891315832 cites W1985724808 @default.
• W2891315832 cites W1988669405 @default.
• W2891315832 cites W2000050780 @default.
• W2891315832 cites W2009240297 @default.
• W2891315832 cites W2015821968 @default.
• W2891315832 cites W2018137678 @default.
• W2891315832 cites W2028500901 @default.
• W2891315832 cites W2037728125 @default.
• W2891315832 cites W2043545239 @default.
• W2891315832 cites W2043730199 @default.
• W2891315832 cites W2049576685 @default.
• W2891315832 cites W2053684353 @default.
• W2891315832 cites W2058918675 @default.
• W2891315832 cites W2068185463 @default.
• W2891315832 cites W2078519009 @default.
• W2891315832 cites W2080032853 @default.
• W2891315832 cites W2100826791 @default.
• W2891315832 cites W2101627975 @default.
• W2891315832 cites W2101751768 @default.
• W2891315832 cites W2105901498 @default.
• W2891315832 cites W2107820972 @default.
• W2891315832 cites W2113872491 @default.
• W2891315832 cites W2121350840 @default.
• W2891315832 cites W2122658576 @default.
• W2891315832 cites W2124109942 @default.
• W2891315832 cites W2129527380 @default.
• W2891315832 cites W2135453449 @default.
• W2891315832 cites W2150308082 @default.
• W2891315832 cites W2153436893 @default.
• W2891315832 cites W2155844132 @default.
• W2891315832 cites W2159439902 @default.
• W2891315832 cites W2161467914 @default.
• W2891315832 cites W2167247319 @default.
• W2891315832 cites W2171775098 @default.
• W2891315832 cites W2263230113 @default.
• W2891315832 cites W2342557539 @default.
• W2891315832 cites W2503586198 @default.
• W2891315832 cites W2515959703 @default.
• W2891315832 cites W2736857166 @default.
• W2891315832 cites W2760969465 @default.
• W2891315832 cites W2770736990 @default.
• W2891315832 cites W2800189919 @default.
• W2891315832 cites W4241678164 @default.
• W2891315832 doi "https://doi.org/10.1073/pnas.1722147115" @default.
• W2891315832 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6187146" @default.
• W2891315832 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30249661" @default.
• W2891315832 hasPublicationYear "2018" @default.
• W2891315832 type Work @default.
• W2891315832 sameAs 2891315832 @default.
• W2891315832 citedByCount "6" @default.
• W2891315832 countsByYear W28913158322019 @default.
• W2891315832 countsByYear W28913158322020 @default.
• W2891315832 countsByYear W28913158322023 @default.
• W2891315832 crossrefType "journal-article" @default.
• W2891315832 hasAuthorship W2891315832A5017441144 @default.
• W2891315832 hasAuthorship W2891315832A5021223273 @default.
• W2891315832 hasAuthorship W2891315832A5029458441 @default.
• W2891315832 hasAuthorship W2891315832A5031343617 @default.
• W2891315832 hasAuthorship W2891315832A5043273992 @default.
• W2891315832 hasAuthorship W2891315832A5051761061 @default.
• W2891315832 hasAuthorship W2891315832A5066987254 @default.
• W2891315832 hasAuthorship W2891315832A5080730606 @default.
• W2891315832 hasBestOaLocation W28913158321 @default.
• W2891315832 hasConcept C104317684 @default.
• W2891315832 hasConcept C14036430 @default.
• W2891315832 hasConcept C143065580 @default.
• W2891315832 hasConcept C167625842 @default.
• W2891315832 hasConcept C177336024 @default.
• W2891315832 hasConcept C2777576037 @default.
• W2891315832 hasConcept C2779222958 @default.
• W2891315832 hasConcept C501734568 @default.
• W2891315832 hasConcept C51639874 @default.
• W2891315832 hasConcept C54355233 @default.
• W2891315832 hasConcept C552990157 @default.
• W2891315832 hasConcept C55493867 @default.
• W2891315832 hasConcept C86339819 @default.
• W2891315832 hasConcept C86803240 @default.

• next