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- W2891323180 abstract "Gelatin added in certain concentration into the density gradient mixtures allows to solidify and melt the gradient reversibly. Such a method was applied to separation of partially disintegrated Escherichia coli VKM B-125 cells in discontinuous sucrose gradient which was optimized to obtain small round nano-cells free from other cell components. Gelatin added to the sucrose solutions had the advantages: i) accurate gradient layers were formed, ii) gelatin did not interfere with the cells and sub-particles during centrifugation, iii) solidified gelatin allowed to cut out the separated cell components, and iv) gelatin allowed to prepare ready-made solidified gradient centrifugation tubes into storage." @default.
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- W2891323180 date "2018-10-01" @default.
- W2891323180 modified "2023-10-01" @default.
- W2891323180 title "Separation of cells in density gradients with temporary solidification of the gradient: Application to Escherichia coli forms obtained by microwave treatment of pure cultures" @default.
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- W2891323180 doi "https://doi.org/10.1016/j.mimet.2018.09.003" @default.
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