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- W2891365730 abstract "Comparative transcriptional analyses require appropriate and precise normalization. Here we describe a modified transcription run-on (TRO) method, named quantitative TRO (qTRO), that allows quantification of nascent transcription activity. The most critical improvement it introduces is a new standardization method for RNA isolation and hybridization steps, enabling transcription activity quantification and comparative biological analysis. We used this technique with chromatin immunoprecipitation to investigate RNA polymerase I (RNAPI) transcription activity and its rDNA gene profiles in Saccharomyces cerevisiae. We designed a set of new oligonucleotide probes complementary to nascent ribosomal RNA (rRNA) transcripts and standardized their hybridization strength. The qTRO method could be successfully implemented to study RNAPI transcription in response to oxidative stress and in two mutant strains with impaired rRNA synthesis." @default.
- W2891365730 created "2018-09-27" @default.
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- W2891365730 date "2018-09-01" @default.
- W2891365730 modified "2023-09-23" @default.
- W2891365730 title "Analysis of rRNA synthesis using quantitative transcription run-on (qTRO) in yeast" @default.
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- W2891365730 doi "https://doi.org/10.2144/btn-2018-0073" @default.
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