Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891366384> ?p ?o ?g. }
- W2891366384 endingPage "16051" @default.
- W2891366384 startingPage "16044" @default.
- W2891366384 abstract "Abstract Macrocyclic and linear hydroxamate siderophores produced by NRPS‐independent siderophore (NIS; NRPS=nonribosomal peptide synthetase) synthetases are important in the bacterial competition for iron, as virulence factors, and as drugs for medical use in humans. Despite their importance, the mechanistic details of NIS synthetases have so far remained obscure. Using synthetic substrate analogues as tools allowed for an interrogation of the mechanism of the two closely related NIS synthetases AvbD and DesD. While AvbD produces macrocyclic homo‐ and heterodimers as native products, DesD is responsible for the synthesis of trimeric desferrioxamines. These enzymes comprise two adjacent binding sites with different substrate selectivities, which direct oligomerization and macrocyclization steps. Exploiting this difference, synthetic substrates were used to invert the native affinities for the sites resulting in switching from trimerization to dimerization reactions for DesD. Based on this work, a comprehensive model explaining the mechanistic details of the reactions and the differences between trimerizing and dimerizing enzymes was developed. Finally, a DesD mutant demonstrated the tuneability of the enzyme's substrate selectivity by only minor changes in the protein sequence. This finding confirms the affinity‐directed mechanism responsible for the iterativity of oligomerization and macrocyclization steps." @default.
- W2891366384 created "2018-09-27" @default.
- W2891366384 creator A5045116743 @default.
- W2891366384 creator A5049490807 @default.
- W2891366384 date "2018-10-17" @default.
- W2891366384 modified "2023-10-14" @default.
- W2891366384 title "Dissecting the Mechanism of Oligomerization and Macrocyclization Reactions of NRPS-Independent Siderophore Synthetases" @default.
- W2891366384 cites W172096104 @default.
- W2891366384 cites W1884751158 @default.
- W2891366384 cites W1970697235 @default.
- W2891366384 cites W198416653 @default.
- W2891366384 cites W1988953378 @default.
- W2891366384 cites W1990871173 @default.
- W2891366384 cites W1993404759 @default.
- W2891366384 cites W1996553642 @default.
- W2891366384 cites W2001236306 @default.
- W2891366384 cites W2020594474 @default.
- W2891366384 cites W2022723077 @default.
- W2891366384 cites W2031090875 @default.
- W2891366384 cites W2034850834 @default.
- W2891366384 cites W2039352898 @default.
- W2891366384 cites W2049318881 @default.
- W2891366384 cites W2054113864 @default.
- W2891366384 cites W2057805033 @default.
- W2891366384 cites W2058332557 @default.
- W2891366384 cites W2060215839 @default.
- W2891366384 cites W2082125514 @default.
- W2891366384 cites W2095923527 @default.
- W2891366384 cites W2099143902 @default.
- W2891366384 cites W2100195325 @default.
- W2891366384 cites W2103287691 @default.
- W2891366384 cites W2106023343 @default.
- W2891366384 cites W2124513776 @default.
- W2891366384 cites W2127681878 @default.
- W2891366384 cites W2137403734 @default.
- W2891366384 cites W2143417865 @default.
- W2891366384 cites W2158068445 @default.
- W2891366384 cites W2234739035 @default.
- W2891366384 cites W2309734820 @default.
- W2891366384 cites W2331266149 @default.
- W2891366384 cites W2557907553 @default.
- W2891366384 cites W2561844505 @default.
- W2891366384 cites W2592529963 @default.
- W2891366384 cites W2606664302 @default.
- W2891366384 cites W2728481641 @default.
- W2891366384 cites W2768735549 @default.
- W2891366384 cites W2772427550 @default.
- W2891366384 cites W2774096277 @default.
- W2891366384 cites W2787961068 @default.
- W2891366384 cites W2791244336 @default.
- W2891366384 cites W2797167677 @default.
- W2891366384 cites W4246629905 @default.
- W2891366384 doi "https://doi.org/10.1002/chem.201803494" @default.
- W2891366384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30182450" @default.
- W2891366384 hasPublicationYear "2018" @default.
- W2891366384 type Work @default.
- W2891366384 sameAs 2891366384 @default.
- W2891366384 citedByCount "20" @default.
- W2891366384 countsByYear W28913663842019 @default.
- W2891366384 countsByYear W28913663842020 @default.
- W2891366384 countsByYear W28913663842021 @default.
- W2891366384 countsByYear W28913663842022 @default.
- W2891366384 countsByYear W28913663842023 @default.
- W2891366384 crossrefType "journal-article" @default.
- W2891366384 hasAuthorship W2891366384A5045116743 @default.
- W2891366384 hasAuthorship W2891366384A5049490807 @default.
- W2891366384 hasConcept C104317684 @default.
- W2891366384 hasConcept C108319773 @default.
- W2891366384 hasConcept C143065580 @default.
- W2891366384 hasConcept C181199279 @default.
- W2891366384 hasConcept C185592680 @default.
- W2891366384 hasConcept C18903297 @default.
- W2891366384 hasConcept C21951064 @default.
- W2891366384 hasConcept C2777289219 @default.
- W2891366384 hasConcept C2777379556 @default.
- W2891366384 hasConcept C2779281246 @default.
- W2891366384 hasConcept C2780283098 @default.
- W2891366384 hasConcept C2994592520 @default.
- W2891366384 hasConcept C553450214 @default.
- W2891366384 hasConcept C55493867 @default.
- W2891366384 hasConcept C71240020 @default.
- W2891366384 hasConcept C86803240 @default.
- W2891366384 hasConceptScore W2891366384C104317684 @default.
- W2891366384 hasConceptScore W2891366384C108319773 @default.
- W2891366384 hasConceptScore W2891366384C143065580 @default.
- W2891366384 hasConceptScore W2891366384C181199279 @default.
- W2891366384 hasConceptScore W2891366384C185592680 @default.
- W2891366384 hasConceptScore W2891366384C18903297 @default.
- W2891366384 hasConceptScore W2891366384C21951064 @default.
- W2891366384 hasConceptScore W2891366384C2777289219 @default.
- W2891366384 hasConceptScore W2891366384C2777379556 @default.
- W2891366384 hasConceptScore W2891366384C2779281246 @default.
- W2891366384 hasConceptScore W2891366384C2780283098 @default.
- W2891366384 hasConceptScore W2891366384C2994592520 @default.
- W2891366384 hasConceptScore W2891366384C553450214 @default.
- W2891366384 hasConceptScore W2891366384C55493867 @default.
- W2891366384 hasConceptScore W2891366384C71240020 @default.
- W2891366384 hasConceptScore W2891366384C86803240 @default.