Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891389916> ?p ?o ?g. }
- W2891389916 endingPage "335" @default.
- W2891389916 startingPage "325" @default.
- W2891389916 abstract "The aim of the current study is to identify bioactive compound from marine endophytic actinomycetes (MEA) isolated from Gulf of Mannar region, Southeast coast of India. Among the isolated actinomycetes, strain GRG 4 exhibited excellent ability to inhibit isolated colistin resistant (CR) Pseudomonas aeruginosa (P. aeruginosa) and Klebsiella pneumoniae (K. pneumoniae), which is a emerging threat to the world. The strain was identified as Streptomyces coeruleorubidus GRG 4 (KY457708), based on morphological, biochemical, phenotypic and genotypic characters. The bioactive metabolites present in the methanolic extract were partially purified by TLC and preparative HPLC. The active HPLC fraction 2 showed 15, 20 mm zone of inhibition against both CR P. aeruginosa and K. pneumoniae respectively. Analytical HPLC and FT-IR results of fraction 2 showed with carbonyl group. Both GC-MS and LC-MS results confirmed that the fraction 2 contained chemical constituents of Bis (2-Ethylhexyl) Phthalate (BEP). The compromised structure with loosely integrated and ruptured cell wall of BEP treated CR bacteria were observed by confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM) at 75 μg/mL of minimum inhibitory concentration (MIC) dose. Further, cytotoxic effect of BEP against A549 human lung cancer cells revealed complete inhibition by cell proliferation and apoptosis was observed at 100 μg/mL in 24 h treatment. In addition, irreversible ROS dependent oxidative damage was clearly observed at the IC50 concentration of BEP. The toxicity of BEP was also studied against Vibrio fischeri (V. fischeri) and found to be highly toxic after 15 and 30 min of treatment. Based on the results it could be concluded that the identified compound BEP is a potent inhibitor for CR bacteria and A549 lung cancer cells." @default.
- W2891389916 created "2018-09-27" @default.
- W2891389916 creator A5000463261 @default.
- W2891389916 creator A5004857228 @default.
- W2891389916 creator A5008788647 @default.
- W2891389916 creator A5036689935 @default.
- W2891389916 creator A5061174068 @default.
- W2891389916 creator A5073866805 @default.
- W2891389916 creator A5090608250 @default.
- W2891389916 date "2018-12-01" @default.
- W2891389916 modified "2023-10-14" @default.
- W2891389916 title "Antibacterial and anticancer potential of marine endophytic actinomycetes Streptomyces coeruleorubidus GRG 4 (KY457708) compound against colistin resistant uropathogens and A549 lung cancer cells" @default.
- W2891389916 cites W1149955938 @default.
- W2891389916 cites W1833157719 @default.
- W2891389916 cites W1861970592 @default.
- W2891389916 cites W1964501401 @default.
- W2891389916 cites W1970326476 @default.
- W2891389916 cites W1979973356 @default.
- W2891389916 cites W2002824835 @default.
- W2891389916 cites W2008200103 @default.
- W2891389916 cites W2010891652 @default.
- W2891389916 cites W2012772199 @default.
- W2891389916 cites W2050886517 @default.
- W2891389916 cites W2061326178 @default.
- W2891389916 cites W2065461553 @default.
- W2891389916 cites W2092263110 @default.
- W2891389916 cites W2095741521 @default.
- W2891389916 cites W2110105875 @default.
- W2891389916 cites W2110218116 @default.
- W2891389916 cites W2114236742 @default.
- W2891389916 cites W2117332590 @default.
- W2891389916 cites W2142531626 @default.
- W2891389916 cites W2154025076 @default.
- W2891389916 cites W2169358526 @default.
- W2891389916 cites W2256166090 @default.
- W2891389916 cites W2259649099 @default.
- W2891389916 cites W2284917903 @default.
- W2891389916 cites W2299402606 @default.
- W2891389916 cites W2333580931 @default.
- W2891389916 cites W2334708274 @default.
- W2891389916 cites W2441234952 @default.
- W2891389916 cites W2472731489 @default.
- W2891389916 cites W2511417222 @default.
- W2891389916 cites W2560797586 @default.
- W2891389916 cites W2576924213 @default.
- W2891389916 cites W2588262608 @default.
- W2891389916 cites W2594376002 @default.
- W2891389916 cites W2606416324 @default.
- W2891389916 cites W2610524905 @default.
- W2891389916 cites W2677752290 @default.
- W2891389916 cites W2753621659 @default.
- W2891389916 cites W2790456939 @default.
- W2891389916 cites W2793501905 @default.
- W2891389916 cites W4210998501 @default.
- W2891389916 cites W590169958 @default.
- W2891389916 cites W837803878 @default.
- W2891389916 doi "https://doi.org/10.1016/j.micpath.2018.09.025" @default.
- W2891389916 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30243551" @default.
- W2891389916 hasPublicationYear "2018" @default.
- W2891389916 type Work @default.
- W2891389916 sameAs 2891389916 @default.
- W2891389916 citedByCount "37" @default.
- W2891389916 countsByYear W28913899162019 @default.
- W2891389916 countsByYear W28913899162020 @default.
- W2891389916 countsByYear W28913899162021 @default.
- W2891389916 countsByYear W28913899162022 @default.
- W2891389916 countsByYear W28913899162023 @default.
- W2891389916 crossrefType "journal-article" @default.
- W2891389916 hasAuthorship W2891389916A5000463261 @default.
- W2891389916 hasAuthorship W2891389916A5004857228 @default.
- W2891389916 hasAuthorship W2891389916A5008788647 @default.
- W2891389916 hasAuthorship W2891389916A5036689935 @default.
- W2891389916 hasAuthorship W2891389916A5061174068 @default.
- W2891389916 hasAuthorship W2891389916A5073866805 @default.
- W2891389916 hasAuthorship W2891389916A5090608250 @default.
- W2891389916 hasConcept C104317684 @default.
- W2891389916 hasConcept C109316439 @default.
- W2891389916 hasConcept C176947019 @default.
- W2891389916 hasConcept C179998833 @default.
- W2891389916 hasConcept C185592680 @default.
- W2891389916 hasConcept C190283241 @default.
- W2891389916 hasConcept C202751555 @default.
- W2891389916 hasConcept C2777058267 @default.
- W2891389916 hasConcept C2777637488 @default.
- W2891389916 hasConcept C2779259085 @default.
- W2891389916 hasConcept C2779920670 @default.
- W2891389916 hasConcept C43617362 @default.
- W2891389916 hasConcept C4937899 @default.
- W2891389916 hasConcept C523546767 @default.
- W2891389916 hasConcept C54355233 @default.
- W2891389916 hasConcept C547475151 @default.
- W2891389916 hasConcept C55493867 @default.
- W2891389916 hasConcept C81729549 @default.
- W2891389916 hasConcept C86803240 @default.
- W2891389916 hasConcept C89423630 @default.
- W2891389916 hasConceptScore W2891389916C104317684 @default.
- W2891389916 hasConceptScore W2891389916C109316439 @default.
- W2891389916 hasConceptScore W2891389916C176947019 @default.