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• W2891395386 abstract "Background: Recently in cancer research has shown that tumors are highly heterogeneous. Cancer developments are driven by specific cells-cancer stem cells (CSCs)-which are also responsible for metastases and drug resistance. Many studies have proved the significant role of miRNAs in controlling the Metastasis in CSCs. So, the present systematic analysis based on literature mining and bioinformatics approaches was performed to find the miRNAs in breast cancer. Methods: After systematic analysis, we found miR-200c and miR-30c as regulators of metastasis in breast cancer. Then, using mammosphere formation in serum-free condition and colony formation assay were conducted to check the stemness properties of MDA-MB231 breast cancer cells. After RNA extraction of mammosphere and primary breast cancer tissue, real-time PCR analysis was performed for metastasis genes (CDH1/2, SNAI1, TWIST1/2 and ZEB1) and the prior selected miRNAs. Finally, differentially expressed genes and miRNAs in mammospheres have been determined and compared with seven malignant breast tissues. Results: Mammospheres demonstrated higher colony-forming ability and has higher expression of metastasis genes in comparison to their parental. Also, they have shown down regulated miR-200c and up regulated of miR-30c. Interestingly, 3 metastatic patients with grade II/III who received neo adjuvant therapy have higher expression of metastasis and have shown down regulated miR-200c and up regulated of miR-30c in comparison to primary breast normal tissue like mammospheres. Conclusions: This study validates the use of breast cancer cell lines as models to elucidate the nature of BCSCs that may represent a novel target for therapy. Additionally, by correlating EMT genes with two miRNAs expression we suggest that simultaneous reduction of miR-200c and increasing in miR-30c could be an indicator for resistance to treatment or secondary metastasis. Legal entity responsible for the study: Department of Stem Cells & Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology & Technology, ACECR, Tehran, Iran. Funding: Department of Stem Cells & Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology & Technology, ACECR, Tehran, Iran. Disclosure: All authors have declared no conflicts of interest." @default.
• W2891395386 created "2018-09-27" @default.
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• W2891395386 date "2018-09-01" @default.
• W2891395386 modified "2023-09-30" @default.
• W2891395386 title "Comparison of expression of microRNAs which regulate metastasis genes in breast cancer stem cells and primary breast cancer tissues" @default.
• W2891395386 doi "https://doi.org/10.1093/annonc/mdy318.023" @default.
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