FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891421302> ?p ?o ?g. }

• W2891421302 abstract "Abstract Biomanufacturing from renewable feedstocks represents a sustainable strategy to offset fossil fuel-based chemical production. One potential biomanufacturing strategy is production of medium chain fatty acids (MCFA) from organic feedstocks using either pure cultures or microbiomes. While the set of microbes in a microbiome can often metabolize more diverse organic materials than a single species, and the role of specific species may be known, knowledge of the carbon and energy flow within and between organisms in MCFA producing microbiomes is only starting to emerge. Here, we integrate metagenomic, metatranscriptomic, and thermodynamic analyses to predict and characterize the metabolic network of an anaerobic microbiome producing MCFA from organic matter derived from lignocellulosic ethanol fermentation conversion residue. A total of 37 high quality (>80% complete, < 10% contamination) metagenome-assembled genomes (MAGs) were recovered from the microbiome and metabolic reconstruction of the 10 most abundant MAGs was performed. Metabolic reconstruction combined with metatranscriptomic analysis predicted that organisms affiliated with Lactobacillus and Coriobacteriaceae degraded carbohydrates and fermented sugars to lactate and acetate. Lachnospiraceae and Eubacteriaceae affiliated organisms were predicted to transform these fermentation products to MCFA. Thermodynamic analyses identified conditions in which H 2 is expected to be either produced or consumed, suggesting a potential role of H 2 partial pressure on MCFA production. From an integrated systems analysis perspective, we propose that MCFA production could be improved if microbiomes are engineered to use homofermentative instead of heterofermentative Lactobacillus , and if MCFA-producing organisms are engineered to preferentially use a thioesterase instead of a CoA transferase as the terminal enzyme in reverse β-oxidation. Importance Mixed communities of microbes play important roles in health, the environment, agriculture, and biotechnology. While tapping the combined activity of organisms within microbiomes may allow the utilization of a wider range of substrate over the use of pure cultures for biomanufacturing, harnessing the metabolism of these mixed cultures remains a major challenge. Here, we predict metabolic functions of bacteria in a microbiome that produces medium-chain fatty acids from a renewable feedstock. Our findings lay the foundation to begin addressing how to engineer and control microbiomes for improved biomanufacturing; to build synthetic mixtures of microbes that produce valuable chemicals from renewable resources; and to better understand microbial communities that contribute to health, agriculture, and the environment." @default.
• W2891421302 created "2018-09-27" @default.
• W2891421302 creator A5017371445 @default.
• W2891421302 creator A5020842663 @default.
• W2891421302 creator A5031617048 @default.
• W2891421302 creator A5039202272 @default.
• W2891421302 creator A5069394957 @default.
• W2891421302 date "2018-09-23" @default.
• W2891421302 modified "2023-09-23" @default.
• W2891421302 title "Metatranscriptomic and thermodynamic insights into medium-chain fatty acid production using an anaerobic microbiome" @default.
• W2891421302 cites W2209197872 @default.
• W2891421302 cites W2550245924 @default.
• W2891421302 cites W2626802773 @default.
• W2891421302 cites W2884536028 @default.
• W2891421302 cites W2891932416 @default.
• W2891421302 doi "https://doi.org/10.1101/424762" @default.
• W2891421302 hasPublicationYear "2018" @default.
• W2891421302 type Work @default.
• W2891421302 sameAs 2891421302 @default.
• W2891421302 citedByCount "1" @default.
• W2891421302 countsByYear W28914213022021 @default.
• W2891421302 crossrefType "posted-content" @default.
• W2891421302 hasAuthorship W2891421302A5017371445 @default.
• W2891421302 hasAuthorship W2891421302A5020842663 @default.
• W2891421302 hasAuthorship W2891421302A5031617048 @default.
• W2891421302 hasAuthorship W2891421302A5039202272 @default.
• W2891421302 hasAuthorship W2891421302A5069394957 @default.
• W2891421302 hasBestOaLocation W28914213021 @default.
• W2891421302 hasConcept C100544194 @default.
• W2891421302 hasConcept C104317684 @default.
• W2891421302 hasConcept C143121216 @default.
• W2891421302 hasConcept C15151743 @default.
• W2891421302 hasConcept C185592680 @default.
• W2891421302 hasConcept C2777695942 @default.
• W2891421302 hasConcept C2779704485 @default.
• W2891421302 hasConcept C31903555 @default.
• W2891421302 hasConcept C42062724 @default.
• W2891421302 hasConcept C55493867 @default.
• W2891421302 hasConcept C60644358 @default.
• W2891421302 hasConcept C86803240 @default.
• W2891421302 hasConceptScore W2891421302C100544194 @default.
• W2891421302 hasConceptScore W2891421302C104317684 @default.
• W2891421302 hasConceptScore W2891421302C143121216 @default.
• W2891421302 hasConceptScore W2891421302C15151743 @default.
• W2891421302 hasConceptScore W2891421302C185592680 @default.
• W2891421302 hasConceptScore W2891421302C2777695942 @default.
• W2891421302 hasConceptScore W2891421302C2779704485 @default.
• W2891421302 hasConceptScore W2891421302C31903555 @default.
• W2891421302 hasConceptScore W2891421302C42062724 @default.
• W2891421302 hasConceptScore W2891421302C55493867 @default.
• W2891421302 hasConceptScore W2891421302C60644358 @default.
• W2891421302 hasConceptScore W2891421302C86803240 @default.
• W2891421302 hasLocation W28914213021 @default.
• W2891421302 hasLocation W28914213022 @default.
• W2891421302 hasLocation W28914213023 @default.
• W2891421302 hasLocation W28914213024 @default.
• W2891421302 hasOpenAccess W2891421302 @default.
• W2891421302 hasPrimaryLocation W28914213021 @default.
• W2891421302 hasRelatedWork W1970472465 @default.
• W2891421302 hasRelatedWork W2096500845 @default.
• W2891421302 hasRelatedWork W2245365787 @default.
• W2891421302 hasRelatedWork W2783050126 @default.
• W2891421302 hasRelatedWork W3001737768 @default.
• W2891421302 hasRelatedWork W3039192128 @default.
• W2891421302 hasRelatedWork W3110332441 @default.
• W2891421302 hasRelatedWork W4206957207 @default.
• W2891421302 hasRelatedWork W4214841476 @default.
• W2891421302 hasRelatedWork W4214945543 @default.
• W2891421302 isParatext "false" @default.
• W2891421302 isRetracted "false" @default.
• W2891421302 magId "2891421302" @default.
• W2891421302 workType "article" @default.