Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891428744> ?p ?o ?g. }
Showing items 1 to 51 of
51
with 100 items per page.
- W2891428744 abstract "Author(s): Wu, Michael Bao Pu | Advisor(s): Zhang, Dong-Er | Abstract: RASSF2 is a powerful pro-apoptotic K-Ras effector that is that is inactivated in many tumors via promoter methylation and has been shown to function as a tumor suppressor in lung, colorectal, and breast cell lines. RASSF2 belongs to the Ras-association domain family (RASSF) of proteins, which are able to engage in homo/hetero-dimerization and interact with common binding partners. In the context of acute myelogenous leukemia (AML), RASSF2 is exclusively downregulated in t(8;21) AML, suggesting that its repression may be essential for t(8;21) leukemia development.In order to further characterize RASSF2’s tumor suppressive role in a leukemic context, we performed a CRISPR/Cas9-mediated knockout of RASSF2 in two non-t(8;21) AML cell lines: HL-60 and U937, generating single cell isolated clonal lines that are RASSF2 wild type, heterozygous, and knockout. Among the clonal cell lines, we assayed for changes in proliferation, apoptosis, cell cycle, and differentiation. We observed that heterozygous knockdown of RASSF2 in the U937 cells resulted in significantly higher (pl.05) proliferation when compared to wild type U937 clonal cells. However, mono- and bi-allelic knockout of RASSF2 did not result in significant differences in differentiation, apoptosis, or cell cycle arrest. In fact, clonal lines of the same genotype were observed to be characteristically variable. Taken together, we demonstrate that RASSF2 knockout is not critical in acute myelogenous leukemia since it does not manifest in a significant functional phenotype that overcame clonal characteristic variation. This suggests that loss of RASSF2 expression leads to the rescue of its function. We also prove that the process of single cell clonal isolation selects for clonal lines that are inherently variable in differentiation, which demonstrates that functional characterizations involving single cell clonal isolation must be performed with a larger pool of samples to account for the characteristic variability of clonal cell lines." @default.
- W2891428744 created "2018-09-27" @default.
- W2891428744 creator A5069240859 @default.
- W2891428744 date "2016-01-01" @default.
- W2891428744 modified "2023-09-24" @default.
- W2891428744 title "Functional characterization of the tumor suppressor RASSF2 in Acute Myelogenous Leukemia via CRISPR/Cas9-mediation" @default.
- W2891428744 hasPublicationYear "2016" @default.
- W2891428744 type Work @default.
- W2891428744 sameAs 2891428744 @default.
- W2891428744 citedByCount "0" @default.
- W2891428744 crossrefType "journal-article" @default.
- W2891428744 hasAuthorship W2891428744A5069240859 @default.
- W2891428744 hasConcept C151730666 @default.
- W2891428744 hasConcept C2778461978 @default.
- W2891428744 hasConcept C2779343474 @default.
- W2891428744 hasConcept C502942594 @default.
- W2891428744 hasConcept C54355233 @default.
- W2891428744 hasConcept C86803240 @default.
- W2891428744 hasConceptScore W2891428744C151730666 @default.
- W2891428744 hasConceptScore W2891428744C2778461978 @default.
- W2891428744 hasConceptScore W2891428744C2779343474 @default.
- W2891428744 hasConceptScore W2891428744C502942594 @default.
- W2891428744 hasConceptScore W2891428744C54355233 @default.
- W2891428744 hasConceptScore W2891428744C86803240 @default.
- W2891428744 hasLocation W28914287441 @default.
- W2891428744 hasOpenAccess W2891428744 @default.
- W2891428744 hasPrimaryLocation W28914287441 @default.
- W2891428744 hasRelatedWork W1493669433 @default.
- W2891428744 hasRelatedWork W1986914857 @default.
- W2891428744 hasRelatedWork W1991912899 @default.
- W2891428744 hasRelatedWork W2010172360 @default.
- W2891428744 hasRelatedWork W2054838258 @default.
- W2891428744 hasRelatedWork W2068940249 @default.
- W2891428744 hasRelatedWork W2145281459 @default.
- W2891428744 hasRelatedWork W2247067509 @default.
- W2891428744 hasRelatedWork W2398619533 @default.
- W2891428744 hasRelatedWork W2412996165 @default.
- W2891428744 hasRelatedWork W2460806560 @default.
- W2891428744 hasRelatedWork W2465690228 @default.
- W2891428744 hasRelatedWork W2508318535 @default.
- W2891428744 hasRelatedWork W2552924338 @default.
- W2891428744 hasRelatedWork W2557223231 @default.
- W2891428744 hasRelatedWork W281337477 @default.
- W2891428744 hasRelatedWork W2922031201 @default.
- W2891428744 hasRelatedWork W2979381396 @default.
- W2891428744 hasRelatedWork W2979408642 @default.
- W2891428744 hasRelatedWork W3082629807 @default.
- W2891428744 isParatext "false" @default.
- W2891428744 isRetracted "false" @default.
- W2891428744 magId "2891428744" @default.
- W2891428744 workType "article" @default.