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- W2891476799 abstract "In this protocol, cells are cultured in the presence of [ 32 P]orthophosphate used by the cell to phosphorylate proteins. Cells must be maintained at 37°C at all times during this procedure. A drop in temperature will significantly reduce cellular metabolic activity and subsequently decrease the uptake of [ 32 P]orthophosphate. Once the cells are labeled, cell lysates are prepared for immunoprecipitation, followed by SDS-PAGE analysis. The gel can be dried directly or transferred onto a nitrocellulose or polyvinylidene fluoride (PVDF) membrane for analysis by western blotting and imaging by autoradiography or phosphorimager." @default.
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- W2891476799 date "2018-09-01" @default.
- W2891476799 modified "2023-10-18" @default.
- W2891476799 title "Metabolic Labeling of Protein Antigens with [<sup>32</sup>P]Orthophosphate" @default.
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- W2891476799 doi "https://doi.org/10.1101/pdb.prot098533" @default.
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