FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891482074> ?p ?o ?g. }

• W2891482074 endingPage "9997" @default.
• W2891482074 startingPage "9992" @default.
• W2891482074 abstract "DNA polymerases have evolved to process the four canonical nucleotides accurately. Nevertheless, these enzymes are also known to process modified nucleotides, which is the key to numerous core biotechnology applications. Processing of modified nucleotides includes incorporation of the modified nucleotide and postincorporation elongation to proceed with the synthesis of the nascent DNA strand. The structural basis for postincorporation elongation is currently unknown. We addressed this issue and successfully crystallized KlenTaq DNA polymerase in six closed ternary complexes containing the enzyme, the modified DNA substrate, and the incoming nucleotide. Each structure shows a high-resolution snapshot of the elongation of a modified primer, where the modification moves from the 3'-primer terminus upstream to the sixth nucleotide in the primer strand. Combining these data with quantum mechanics/molecular mechanics calculations and biochemical studies elucidates how the enzyme and the modified substrate mutually modulate their conformations without compromising the enzyme's activity significantly. The study highlights the plasticity of the system as origin of the broad substrate properties of DNA polymerases and facilitates the design of improved systems." @default.
• W2891482074 created "2018-09-27" @default.
• W2891482074 creator A5001031775 @default.
• W2891482074 creator A5010133877 @default.
• W2891482074 creator A5011269879 @default.
• W2891482074 creator A5012342782 @default.
• W2891482074 creator A5035525469 @default.
• W2891482074 date "2018-09-17" @default.
• W2891482074 modified "2023-10-18" @default.
• W2891482074 title "Snapshots of a modified nucleotide moving through the confines of a DNA polymerase" @default.
• W2891482074 cites W1970080065 @default.
• W2891482074 cites W1986326400 @default.
• W2891482074 cites W1994567145 @default.
• W2891482074 cites W2001825475 @default.
• W2891482074 cites W2012016911 @default.
• W2891482074 cites W2013059500 @default.
• W2891482074 cites W2026398206 @default.
• W2891482074 cites W2026758416 @default.
• W2891482074 cites W2027011093 @default.
• W2891482074 cites W2052484845 @default.
• W2891482074 cites W2053792903 @default.
• W2891482074 cites W2054423031 @default.
• W2891482074 cites W2054525948 @default.
• W2891482074 cites W2057141789 @default.
• W2891482074 cites W2072461007 @default.
• W2891482074 cites W2073040109 @default.
• W2891482074 cites W2091984642 @default.
• W2891482074 cites W2092013032 @default.
• W2891482074 cites W2093322394 @default.
• W2891482074 cites W2097255042 @default.
• W2891482074 cites W2098273801 @default.
• W2891482074 cites W2103272211 @default.
• W2891482074 cites W2110416817 @default.
• W2891482074 cites W2118526609 @default.
• W2891482074 cites W2122464209 @default.
• W2891482074 cites W2123321071 @default.
• W2891482074 cites W2128450975 @default.
• W2891482074 cites W2146296036 @default.
• W2891482074 cites W2149410625 @default.
• W2891482074 cites W2153508349 @default.
• W2891482074 cites W2153792822 @default.
• W2891482074 cites W2156643308 @default.
• W2891482074 cites W2164389987 @default.
• W2891482074 cites W2167092133 @default.
• W2891482074 cites W2291221808 @default.
• W2891482074 cites W2334213118 @default.
• W2891482074 cites W2340001130 @default.
• W2891482074 cites W2367508821 @default.
• W2891482074 cites W2396849069 @default.
• W2891482074 cites W2460559503 @default.
• W2891482074 cites W2558307411 @default.
• W2891482074 cites W2585093856 @default.
• W2891482074 cites W2767332703 @default.
• W2891482074 cites W2797942267 @default.
• W2891482074 cites W2808574630 @default.
• W2891482074 cites W4254945764 @default.
• W2891482074 doi "https://doi.org/10.1073/pnas.1811518115" @default.
• W2891482074 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6176618" @default.
• W2891482074 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30224478" @default.
• W2891482074 hasPublicationYear "2018" @default.
• W2891482074 type Work @default.
• W2891482074 sameAs 2891482074 @default.
• W2891482074 citedByCount "22" @default.
• W2891482074 countsByYear W28914820742019 @default.
• W2891482074 countsByYear W28914820742020 @default.
• W2891482074 countsByYear W28914820742021 @default.
• W2891482074 countsByYear W28914820742022 @default.
• W2891482074 countsByYear W28914820742023 @default.
• W2891482074 crossrefType "journal-article" @default.
• W2891482074 hasAuthorship W2891482074A5001031775 @default.
• W2891482074 hasAuthorship W2891482074A5010133877 @default.
• W2891482074 hasAuthorship W2891482074A5011269879 @default.
• W2891482074 hasAuthorship W2891482074A5012342782 @default.
• W2891482074 hasAuthorship W2891482074A5035525469 @default.
• W2891482074 hasBestOaLocation W28914820741 @default.
• W2891482074 hasConcept C104317684 @default.
• W2891482074 hasConcept C178790620 @default.
• W2891482074 hasConcept C185592680 @default.
• W2891482074 hasConcept C2756471 @default.
• W2891482074 hasConcept C2777563447 @default.
• W2891482074 hasConcept C512185932 @default.
• W2891482074 hasConcept C54355233 @default.
• W2891482074 hasConcept C552990157 @default.
• W2891482074 hasConcept C55493867 @default.
• W2891482074 hasConcept C82381507 @default.
• W2891482074 hasConcept C86803240 @default.
• W2891482074 hasConceptScore W2891482074C104317684 @default.
• W2891482074 hasConceptScore W2891482074C178790620 @default.
• W2891482074 hasConceptScore W2891482074C185592680 @default.
• W2891482074 hasConceptScore W2891482074C2756471 @default.
• W2891482074 hasConceptScore W2891482074C2777563447 @default.
• W2891482074 hasConceptScore W2891482074C512185932 @default.
• W2891482074 hasConceptScore W2891482074C54355233 @default.
• W2891482074 hasConceptScore W2891482074C552990157 @default.
• W2891482074 hasConceptScore W2891482074C55493867 @default.
• W2891482074 hasConceptScore W2891482074C82381507 @default.
• W2891482074 hasConceptScore W2891482074C86803240 @default.
• W2891482074 hasIssue "40" @default.

• next