FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891524480> ?p ?o ?g. }

• W2891524480 abstract "In a phosphorus (P) deficient animal diet, phytase supplementation improves the bioavailabilities of P as well as other nutrients by phytate hydrolysis andindirectly, plays a role in biological function of many metabolic processes. In consequence, hypothetically, chicken health in terms of immune responses associated with hematological parameters, blood biochemical constituents and live body weight might be influenced. Phytase production commercially,focuses only on the soil fungus Aspergillus, but many possible sources ofmicrobial novel phytases remain unexplored. In Malaysia, around 30 strains of potential phytase producing soil bacteria were successfully harvested and they show good enzymatic activities and characteristics favouring physiology of chicken gut. In addition, some bacterial phytases, especially those of the genera Bacillus and Enterobacter, exhibits a pH optimum ranging from 6.0 to8.0, close to the physiological pH of the stomach of chicken. Newcastle disease (ND) and infectious bursal disease (IBD) are the most important diseases for poultry worldwide, which can cause huge economic losses in thepoultry industry. In Malaysia, therefore, the possibility of using the locally produced bacterial phytase from Enterobacter sakazakii ASUA273 in broilers fed low P diet (0.19%) and vaccinated against ND and IBD could be justified. The objective of the study was to determine the effects of microbial phytases on humoral immunity and blood characteristics in association with the livebody weights of broilers vaccinated with ND and IBD vaccines. Five experiments (Experiment l, ll, lll, IV and V) were carried out. The first three trials (Experiment l, ll and lll) were carried out in broilers fed low P diet with four doses (0 FTU/kg-1, 500 FTU/kg-1, 1000 FTU/kg-1, and 1500 FTU/kg-1 of diet) of local bacterial phytase grouped as T0 (control), T1, T2, and T3,accordingly on ND, IBD, and both of ND and IBD vaccinations, respectively. In each trial, 180 day-old-male broilers were allocated to four treatmentgroups with 12 cages comprising three replicates, each cage containing 15 birds. The last two trials (Experiment lV and V) were conducted with broiler chickens fed the same diet, with two doses (0 FTU/kg-1 and 1500 FTU/kg-1 of diet) of Natuphos® grouped as T0 (control) and T1, accordingly on ND and IBD vaccinations, respectively. Ninety (92) day-old-male broilers per trial were randomly assigned into two treatments, each contained three replicates of 15 chicks each. They were maintained on formulated experimental basal diet based on available phosphorus (0.19%), lasted up to six weeks of agewith feed, and water made attainable for ad libitum consumption. Birds received two doses of ND vaccines (ND ‘V4 HR’) at day-old and 21 day-old,respectively and one dose of IBD vaccine (IBD UPM93) at 10 day-old. Twobirds were randomly selected weekly, from each treated group (8 and 4 birds per replicate were selected from experiments using local phytase and Natuphos® supplementation, respectively) and live body weights weremeasured. These birds were then slaughtered for blood collection to prepare serum for quantification of antibody (Ab) titers, IgM, and IgG and jejunal fluidwere collected to quantify IgA throughout the experiment. At the end of experiment, blood was furthermore collected for determining the complete hemogram and blood biochemistry. Antibody titers (ND and IBD), IgM, IgG,and IgA were detected by ELISA using commercial kits. Although a hematology analyzer using commercial reagents measured the complete hemogram, other parameters (differential leukocyte count (DLC), packed cellvolume (PCV), icterus index, and total plasma protein) were determined manually. All blood biochemical constituents were determined with the help of a chemistry analyzer. Data of humoral immunity with live body weights,and blood characteristics were analyzed based on factorial arrangement (treatments × weeks) of completely randomized design (CRD) and CRD,respectively. Results of humoral immunity of vaccinated broilers showed thatserum Ab titers (ND and IBD), IgM, and IgG contents did not increase by phytase supplementation in low P diet. However, mucosal secretory IgA concentrations of vaccinated birds increased consistently and significantly(P<0.05) with increasing phytase doses throughout the experiments. Results of live body weights of broilers showed that body weights were linearly and significantly (P<0.05) increased to graded levels of phytase supplementation at weekly intervals. Cumulative effects of mucosal IgA contents and live body weights of broilers also showed the significant interaction between effects of phytase levels and effects of weeks. Overall, phytase dose at 1500 FTU/kg-1 of diet and over the age of 6 weeks showed the best performance. On the overall, findings on complete hemogram and blood biochemical constituents did not show any consistent and significant (P<0.05) difference that would suggest that phytase supplementation in corn-soybean based P deficient dietaffected the health of broilers. It was therefore, concluded that the locally produced bacterial phytase obtained from Enterobacter sakazakii273 couldbe as effective as the commercially produced fungal phytase (Natuphos®). Further researches are recommended to determine the optimum level of available P in order to produce maximum performances (body weight gain,feed intake, feed conversion ratio, bone mineralization, mineral retention, and P excretion in the environment) using larger number of chickens. The cellmediatedimmunity in broilers vaccinated against ND and IBD vaccines should also be measured to assess the real effect on immune response by local phytase supplementation in low P diet. In addition, phytase fromASUIA273 can be used to a diet to determine the impacts on vitamin-D,parathormone, glucocorticoids, and thyroids in animal body." @default.
• W2891524480 created "2018-09-27" @default.
• W2891524480 creator A5084587943 @default.
• W2891524480 date "2014-04-01" @default.
• W2891524480 modified "2023-09-27" @default.
• W2891524480 title "Effects of locally produced microbial phytase on humoral immunity and blood characteristics in broilers vaccinated against newcastle and infectious bursal diseases" @default.
• W2891524480 hasPublicationYear "2014" @default.
• W2891524480 type Work @default.
• W2891524480 sameAs 2891524480 @default.
• W2891524480 citedByCount "0" @default.
• W2891524480 crossrefType "dissertation" @default.
• W2891524480 hasAuthorship W2891524480A5084587943 @default.
• W2891524480 hasConcept C104317684 @default.
• W2891524480 hasConcept C109551439 @default.
• W2891524480 hasConcept C150903083 @default.
• W2891524480 hasConcept C181199279 @default.
• W2891524480 hasConcept C203014093 @default.
• W2891524480 hasConcept C2522874641 @default.
• W2891524480 hasConcept C2777810641 @default.
• W2891524480 hasConcept C2778116459 @default.
• W2891524480 hasConcept C2778883795 @default.
• W2891524480 hasConcept C2778983983 @default.
• W2891524480 hasConcept C2779341262 @default.
• W2891524480 hasConcept C2780255968 @default.
• W2891524480 hasConcept C2780289900 @default.
• W2891524480 hasConcept C31903555 @default.
• W2891524480 hasConcept C509243982 @default.
• W2891524480 hasConcept C523546767 @default.
• W2891524480 hasConcept C54355233 @default.
• W2891524480 hasConcept C547475151 @default.
• W2891524480 hasConcept C55493867 @default.
• W2891524480 hasConcept C60987743 @default.
• W2891524480 hasConcept C86803240 @default.
• W2891524480 hasConcept C8891405 @default.
• W2891524480 hasConcept C89423630 @default.
• W2891524480 hasConceptScore W2891524480C104317684 @default.
• W2891524480 hasConceptScore W2891524480C109551439 @default.
• W2891524480 hasConceptScore W2891524480C150903083 @default.
• W2891524480 hasConceptScore W2891524480C181199279 @default.
• W2891524480 hasConceptScore W2891524480C203014093 @default.
• W2891524480 hasConceptScore W2891524480C2522874641 @default.
• W2891524480 hasConceptScore W2891524480C2777810641 @default.
• W2891524480 hasConceptScore W2891524480C2778116459 @default.
• W2891524480 hasConceptScore W2891524480C2778883795 @default.
• W2891524480 hasConceptScore W2891524480C2778983983 @default.
• W2891524480 hasConceptScore W2891524480C2779341262 @default.
• W2891524480 hasConceptScore W2891524480C2780255968 @default.
• W2891524480 hasConceptScore W2891524480C2780289900 @default.
• W2891524480 hasConceptScore W2891524480C31903555 @default.
• W2891524480 hasConceptScore W2891524480C509243982 @default.
• W2891524480 hasConceptScore W2891524480C523546767 @default.
• W2891524480 hasConceptScore W2891524480C54355233 @default.
• W2891524480 hasConceptScore W2891524480C547475151 @default.
• W2891524480 hasConceptScore W2891524480C55493867 @default.
• W2891524480 hasConceptScore W2891524480C60987743 @default.
• W2891524480 hasConceptScore W2891524480C86803240 @default.
• W2891524480 hasConceptScore W2891524480C8891405 @default.
• W2891524480 hasConceptScore W2891524480C89423630 @default.
• W2891524480 hasLocation W28915244801 @default.
• W2891524480 hasOpenAccess W2891524480 @default.
• W2891524480 hasPrimaryLocation W28915244801 @default.
• W2891524480 hasRelatedWork W2106584691 @default.
• W2891524480 hasRelatedWork W2112668506 @default.
• W2891524480 hasRelatedWork W2115717910 @default.
• W2891524480 hasRelatedWork W2131362736 @default.
• W2891524480 hasRelatedWork W2162735287 @default.
• W2891524480 hasRelatedWork W2167903119 @default.
• W2891524480 hasRelatedWork W2174767992 @default.
• W2891524480 hasRelatedWork W2186842728 @default.
• W2891524480 hasRelatedWork W2265386243 @default.
• W2891524480 hasRelatedWork W2352878977 @default.
• W2891524480 hasRelatedWork W2586471531 @default.
• W2891524480 hasRelatedWork W2596344206 @default.
• W2891524480 hasRelatedWork W2597323089 @default.
• W2891524480 hasRelatedWork W2747681877 @default.
• W2891524480 hasRelatedWork W2787038648 @default.
• W2891524480 hasRelatedWork W3004601846 @default.
• W2891524480 hasRelatedWork W3019547765 @default.
• W2891524480 hasRelatedWork W3133655185 @default.
• W2891524480 hasRelatedWork W887240860 @default.
• W2891524480 hasRelatedWork W2611005816 @default.
• W2891524480 isParatext "false" @default.
• W2891524480 isRetracted "false" @default.
• W2891524480 magId "2891524480" @default.
• W2891524480 workType "dissertation" @default.