Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891551181> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2891551181 endingPage "42" @default.
- W2891551181 startingPage "33" @default.
- W2891551181 abstract "We describe a method for polyethyleneimine (PEI) mediated transient transfection of suspension-adapted Chinese hamster ovary (CHO-DG44) cells for protein expression applicable at scales from 2 mL to 2 L. The method involves transfection at a high cell density (5 × 106 cells/mL) by direct addition of plasmid DNA (pDNA) and PEI to the culture and subsequent incubation at 31 °C with agitation by orbital shaking. This method requires 0.3 mg/L of coding pDNA, 2.7 mg/L of nonspecific (filler) DNA and 15 mg/L of PEI. The production phase is performed at 31 °C in the presence of 0.25% N,N-dimethylacetamide (DMA). We also provide information on culture vessel options, recommended working volumes, and recommended shaking speeds for transfections at scales from 2 mL to 2 L." @default.
- W2891551181 created "2018-09-27" @default.
- W2891551181 creator A5087273668 @default.
- W2891551181 date "2018-01-01" @default.
- W2891551181 modified "2023-10-18" @default.
- W2891551181 title "PEI-Mediated Transient Gene Expression in CHO Cells" @default.
- W2891551181 cites W1875527972 @default.
- W2891551181 cites W1940198378 @default.
- W2891551181 cites W1979578972 @default.
- W2891551181 cites W1986329449 @default.
- W2891551181 cites W1992273316 @default.
- W2891551181 cites W2032901225 @default.
- W2891551181 cites W2075251342 @default.
- W2891551181 cites W2085806334 @default.
- W2891551181 cites W2116213570 @default.
- W2891551181 cites W2186467938 @default.
- W2891551181 cites W2598907213 @default.
- W2891551181 cites W2612548846 @default.
- W2891551181 doi "https://doi.org/10.1007/978-1-4939-8730-6_3" @default.
- W2891551181 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30242678" @default.
- W2891551181 hasPublicationYear "2018" @default.
- W2891551181 type Work @default.
- W2891551181 sameAs 2891551181 @default.
- W2891551181 citedByCount "4" @default.
- W2891551181 countsByYear W28915511812021 @default.
- W2891551181 countsByYear W28915511812022 @default.
- W2891551181 countsByYear W28915511812023 @default.
- W2891551181 crossrefType "book-chapter" @default.
- W2891551181 hasAuthorship W2891551181A5087273668 @default.
- W2891551181 hasConcept C104317684 @default.
- W2891551181 hasConcept C150194340 @default.
- W2891551181 hasConcept C153911025 @default.
- W2891551181 hasConcept C175656101 @default.
- W2891551181 hasConcept C182420341 @default.
- W2891551181 hasConcept C185592680 @default.
- W2891551181 hasConcept C190283241 @default.
- W2891551181 hasConcept C22744801 @default.
- W2891551181 hasConcept C25642318 @default.
- W2891551181 hasConcept C54009773 @default.
- W2891551181 hasConcept C54355233 @default.
- W2891551181 hasConcept C552990157 @default.
- W2891551181 hasConcept C55493867 @default.
- W2891551181 hasConcept C81885089 @default.
- W2891551181 hasConcept C86803240 @default.
- W2891551181 hasConcept C91779695 @default.
- W2891551181 hasConcept C95444343 @default.
- W2891551181 hasConceptScore W2891551181C104317684 @default.
- W2891551181 hasConceptScore W2891551181C150194340 @default.
- W2891551181 hasConceptScore W2891551181C153911025 @default.
- W2891551181 hasConceptScore W2891551181C175656101 @default.
- W2891551181 hasConceptScore W2891551181C182420341 @default.
- W2891551181 hasConceptScore W2891551181C185592680 @default.
- W2891551181 hasConceptScore W2891551181C190283241 @default.
- W2891551181 hasConceptScore W2891551181C22744801 @default.
- W2891551181 hasConceptScore W2891551181C25642318 @default.
- W2891551181 hasConceptScore W2891551181C54009773 @default.
- W2891551181 hasConceptScore W2891551181C54355233 @default.
- W2891551181 hasConceptScore W2891551181C552990157 @default.
- W2891551181 hasConceptScore W2891551181C55493867 @default.
- W2891551181 hasConceptScore W2891551181C81885089 @default.
- W2891551181 hasConceptScore W2891551181C86803240 @default.
- W2891551181 hasConceptScore W2891551181C91779695 @default.
- W2891551181 hasConceptScore W2891551181C95444343 @default.
- W2891551181 hasLocation W28915511811 @default.
- W2891551181 hasLocation W28915511812 @default.
- W2891551181 hasOpenAccess W2891551181 @default.
- W2891551181 hasPrimaryLocation W28915511811 @default.
- W2891551181 hasRelatedWork W119418178 @default.
- W2891551181 hasRelatedWork W2019099201 @default.
- W2891551181 hasRelatedWork W2058797471 @default.
- W2891551181 hasRelatedWork W2083274803 @default.
- W2891551181 hasRelatedWork W2313181124 @default.
- W2891551181 hasRelatedWork W2357235844 @default.
- W2891551181 hasRelatedWork W2364175713 @default.
- W2891551181 hasRelatedWork W2368733723 @default.
- W2891551181 hasRelatedWork W3138321581 @default.
- W2891551181 hasRelatedWork W4283802959 @default.
- W2891551181 isParatext "false" @default.
- W2891551181 isRetracted "false" @default.
- W2891551181 magId "2891551181" @default.
- W2891551181 workType "book-chapter" @default.