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• W2891551516 abstract "To ascertain whether red light, known to enhance mitochondrial function, can blunt a blue light insult to ARPE19 cells in culture.Semi-confluent ARPE19 cells cultured in 10% FBS were subjected to various regimes of treatment with blue (465-475 nm, 800 lux, 26 W/m2 ) and red (625-635 nm, 950 lux, 6.5 W/m2 ) light, as well as with toxins that inactivate specific enzymes associated with mitochondrial oxidative phosphorylation. Cultures were then analysed for cell viability (MTT assay), mitochondrial status (JC-1), ROS formation, immunocytochemistry and the activation of specific proteins by electrophoresis/Western blotting. In addition, ARPE19 cells were cultured in polycarbonate membrane inserts in culture medium containing 1% FBS. Such cultures were exposed to cycles of red, blue or a combination of red and blue light for up to 6 weeks. Culture medium was changed and the trans-epithelium membrane resistance (TER) of the inserts-containing cells was measured twice weekly.ARPE19 cells in culture are affected negatively when exposed to blue light. This is indicated by a loss of viability, a depolarization of their mitochondria and a stimulation of ROS. Moreover, blue light causes an up-regulation of HO-1 and phospho-p-38-MAPK and a cleavage of apoptosis inhibitory factor, proteins which are all known to be activated during cell death. All of these negative effects of blue light are significantly blunted by the red light administered after the blue light insult in each case. ARPE19 cell loss of viability and mitochondrial potential caused by toxins that inhibit specific mitochondrial enzyme complexes was additive to an insult delivered by blue light in each case. After a time, ARPE19 cells in culture express the tight junction protein ZO-1, which is affected by blue light. The development of tight junctions between ARPE19 cells grown in inserts reached a steady peak of resistance after about 40 days and then increased very slightly over the next 40 days when still in darkness. However, maximum resistance was significantly attenuated, when cultures were treated with cycles of blue light after the initial 40 days in the dark and counteracted significantly when the blue light cycle insult was combined with red light.Blue light affects mitochondrial function and also the development tight junctions between ARPE19 cells, which results in a loss of cell viability. Importantly, red light delivered after a blue light insult is significantly blunted. These findings argue for the therapeutic use of red light as a noninvasive procedure to attenuate insults caused by blue light and other insults to retinal pigment epithelial cell mitochondria that are likely to occur in age-related macular degeneration." @default.
• W2891551516 created "2018-09-27" @default.
• W2891551516 creator A5034726740 @default.
• W2891551516 creator A5056124686 @default.
• W2891551516 creator A5069352403 @default.
• W2891551516 creator A5089713407 @default.
• W2891551516 date "2018-09-09" @default.
• W2891551516 modified "2023-09-30" @default.
• W2891551516 title "Blue light negatively affects the survival of ARPE19 cells through an action on their mitochondria and blunted by red light" @default.
• W2891551516 cites W1526655781 @default.
• W2891551516 cites W1615358991 @default.
• W2891551516 cites W1892356714 @default.
• W2891551516 cites W1928416889 @default.
• W2891551516 cites W1964637189 @default.
• W2891551516 cites W1964952274 @default.
• W2891551516 cites W1966763936 @default.
• W2891551516 cites W1969185891 @default.
• W2891551516 cites W1973679435 @default.
• W2891551516 cites W1976805974 @default.
• W2891551516 cites W1984215221 @default.
• W2891551516 cites W1985811338 @default.
• W2891551516 cites W1988655325 @default.
• W2891551516 cites W1991743283 @default.
• W2891551516 cites W1994133882 @default.
• W2891551516 cites W1995103965 @default.
• W2891551516 cites W1996953529 @default.
• W2891551516 cites W1998262397 @default.
• W2891551516 cites W1998797995 @default.
• W2891551516 cites W2001164337 @default.
• W2891551516 cites W2007338505 @default.
• W2891551516 cites W2009481357 @default.
• W2891551516 cites W2011823018 @default.
• W2891551516 cites W2012832087 @default.
• W2891551516 cites W2014513835 @default.
• W2891551516 cites W2018398696 @default.
• W2891551516 cites W2019833658 @default.
• W2891551516 cites W2020134182 @default.
• W2891551516 cites W2023838096 @default.
• W2891551516 cites W2028610920 @default.
• W2891551516 cites W2034184306 @default.
• W2891551516 cites W2035360638 @default.
• W2891551516 cites W2035490715 @default.
• W2891551516 cites W2036903951 @default.
• W2891551516 cites W2044141700 @default.
• W2891551516 cites W2047393488 @default.
• W2891551516 cites W2049871886 @default.
• W2891551516 cites W2050971607 @default.
• W2891551516 cites W2056293291 @default.
• W2891551516 cites W2056620406 @default.
• W2891551516 cites W2056659132 @default.
• W2891551516 cites W2056875520 @default.
• W2891551516 cites W2058108015 @default.
• W2891551516 cites W2059691962 @default.
• W2891551516 cites W2061017616 @default.
• W2891551516 cites W2067065591 @default.
• W2891551516 cites W2068104033 @default.
• W2891551516 cites W2071007484 @default.
• W2891551516 cites W2074329998 @default.
• W2891551516 cites W2075551125 @default.
• W2891551516 cites W2079825767 @default.
• W2891551516 cites W2085641738 @default.
• W2891551516 cites W2085960334 @default.
• W2891551516 cites W2086536717 @default.
• W2891551516 cites W2087146423 @default.
• W2891551516 cites W2087790613 @default.
• W2891551516 cites W2089017520 @default.
• W2891551516 cites W2091487004 @default.
• W2891551516 cites W2093452444 @default.
• W2891551516 cites W2093888690 @default.
• W2891551516 cites W2094302526 @default.
• W2891551516 cites W2094857141 @default.
• W2891551516 cites W2114918609 @default.
• W2891551516 cites W2121833062 @default.
• W2891551516 cites W2123274056 @default.
• W2891551516 cites W2124122523 @default.
• W2891551516 cites W2125324546 @default.
• W2891551516 cites W2133177101 @default.
• W2891551516 cites W2135661251 @default.
• W2891551516 cites W2135700152 @default.
• W2891551516 cites W2136359299 @default.
• W2891551516 cites W2136494048 @default.
• W2891551516 cites W2137745164 @default.
• W2891551516 cites W2143249129 @default.
• W2891551516 cites W2144839999 @default.
• W2891551516 cites W2145071690 @default.
• W2891551516 cites W2148200348 @default.
• W2891551516 cites W2149848093 @default.
• W2891551516 cites W2167340008 @default.
• W2891551516 cites W2169164629 @default.
• W2891551516 cites W2177010324 @default.
• W2891551516 cites W2291689294 @default.
• W2891551516 cites W2337485948 @default.
• W2891551516 cites W2341577039 @default.
• W2891551516 cites W2396811347 @default.
• W2891551516 cites W2411031570 @default.
• W2891551516 cites W2467527170 @default.
• W2891551516 cites W2538984373 @default.
• W2891551516 cites W2560822119 @default.
• W2891551516 cites W2563569933 @default.
• W2891551516 cites W2602573314 @default.

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