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• W2891571774 abstract "Significance While biological utilization of one-carbon (C1) compounds has attracted much attention, previous studies have focused mainly on the utilization of CO 2 . Here, we report development of Escherichia coli strains capable of assimilating formic acid (FA) and CO 2 through the C1 assimilation pathway, synthesizing pyruvate from FA and CO 2 by establishing the reconstructed tetrahydrofolate cycle and the reverse glycine cleavage pathway. To generate energy and redox while using less glucose, a heterologous formate dehydrogenase was introduced together with the C1 assimilation pathway. The resulting strain could utilize FA and CO 2 as sole carbon sources for sustaining growth. This work demonstrates that the combined use of the C1 assimilation pathway and formate dehydrogenase allows E. coli to utilize FA and CO 2 efficiently." @default.
• W2891571774 created "2018-09-27" @default.
• W2891571774 creator A5008430104 @default.
• W2891571774 creator A5082427477 @default.
• W2891571774 date "2018-09-17" @default.
• W2891571774 modified "2023-10-03" @default.
• W2891571774 title "Assimilation of formic acid and CO ₂ by engineered <i>Escherichia coli</i> equipped with reconstructed one-carbon assimilation pathways" @default.
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• W2891571774 doi "https://doi.org/10.1073/pnas.1810386115" @default.
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