FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891573044> ?p ?o ?g. }

• W2891573044 abstract "ABSTRACT The gastrointestinal colonizer Enterococcus faecium is a leading cause of hospital-acquired infections. Multidrug-resistant (MDR) E. faecium are particularly concerning for infection treatment. Previous comparative genomic studies revealed that subspecies referred to as Clade A and Clade B exist within E. faecium . MDR E. faecium belong to Clade A, while Clade B consists of drug-susceptible fecal commensal E. faecium . Isolates from Clade A are further grouped into two sub-clades, A1 and A2. In general, Clade A1 isolates are hospital epidemic isolates whereas Clade A2 isolates are isolates from animals and sporadic human infections. Such phylogenetic separation indicates that reduced gene exchange occurs between the clades. We hypothesize that endogenous barriers to gene exchange exist between E. faecium clades. Restriction-modification (R-M) systems are such barriers in other microbes. We utilized bioinformatics analysis coupled with second generation and third generation deep sequencing platforms to characterize the methylome of two representative E. faecium strains, one from Clade A1 and one from Clade B. We identified a Type I R-M system that is Clade A1-specific, is active for DNA methylation, and significantly reduces transformability of Clade A1 E. faecium . Based on our results, we conclude that R-M systems act as barriers to horizontal gene exchange in E. faecium and propose that R-M systems contribute to E. faecium subspecies separation. IMPORTANCE Enterococcus faecium is a leading cause of hospital-acquired infections around the world. Rising antibiotic resistance in certain E. faecium lineages leaves fewer treatment options. The overarching aim of the attached work was to determine whether restriction-modification (R-M) systems contribute to the structure of the E. faecium species, wherein hospital-epidemic and non-hospital-epidemic isolates have distinct evolutionary histories and highly resolved clade structures. R-M provides bacteria with a type of innate immunity to horizontal gene transfer (HGT). We identified a Type I R-M system that is enriched in the hospital-epidemic clade and determined that it is active for DNA modification activity and significantly impacts HGT. Overall, this work is important because it provides a mechanism for the observed clade structure of E. faecium as well as a mechanism for facilitated gene exchange among hospital-epidemic E. faecium ." @default.
• W2891573044 created "2018-09-27" @default.
• W2891573044 creator A5000280826 @default.
• W2891573044 creator A5002492478 @default.
• W2891573044 creator A5051244336 @default.
• W2891573044 creator A5058417247 @default.
• W2891573044 creator A5063841588 @default.
• W2891573044 date "2018-09-05" @default.
• W2891573044 modified "2023-09-24" @default.
• W2891573044 title "A Type I Restriction-Modification System Associated withEnterococcus faeciumSubspecies Separation" @default.
• W2891573044 cites W1545338345 @default.
• W2891573044 cites W1553436411 @default.
• W2891573044 cites W1586342495 @default.
• W2891573044 cites W1966595658 @default.
• W2891573044 cites W1969701499 @default.
• W2891573044 cites W1972921915 @default.
• W2891573044 cites W1973437578 @default.
• W2891573044 cites W1984783889 @default.
• W2891573044 cites W1990152860 @default.
• W2891573044 cites W1991471459 @default.
• W2891573044 cites W1991634127 @default.
• W2891573044 cites W2008951118 @default.
• W2891573044 cites W2022316530 @default.
• W2891573044 cites W2022496007 @default.
• W2891573044 cites W2025361601 @default.
• W2891573044 cites W2042177152 @default.
• W2891573044 cites W2057744551 @default.
• W2891573044 cites W2064577406 @default.
• W2891573044 cites W2069332918 @default.
• W2891573044 cites W2075969575 @default.
• W2891573044 cites W2080945645 @default.
• W2891573044 cites W2084369308 @default.
• W2891573044 cites W2085492504 @default.
• W2891573044 cites W2104371716 @default.
• W2891573044 cites W2108058966 @default.
• W2891573044 cites W2109117147 @default.
• W2891573044 cites W2118436958 @default.
• W2891573044 cites W2118473805 @default.
• W2891573044 cites W2121453804 @default.
• W2891573044 cites W2123755502 @default.
• W2891573044 cites W2127266712 @default.
• W2891573044 cites W2130063710 @default.
• W2891573044 cites W2130607415 @default.
• W2891573044 cites W2131374955 @default.
• W2891573044 cites W2132341951 @default.
• W2891573044 cites W2146155543 @default.
• W2891573044 cites W2152900584 @default.
• W2891573044 cites W2157009395 @default.
• W2891573044 cites W2163330376 @default.
• W2891573044 cites W2169297879 @default.
• W2891573044 cites W2171434257 @default.
• W2891573044 cites W2264366671 @default.
• W2891573044 cites W2412140074 @default.
• W2891573044 cites W4247447075 @default.
• W2891573044 doi "https://doi.org/10.1101/408914" @default.
• W2891573044 hasPublicationYear "2018" @default.
• W2891573044 type Work @default.
• W2891573044 sameAs 2891573044 @default.
• W2891573044 citedByCount "0" @default.
• W2891573044 crossrefType "posted-content" @default.
• W2891573044 hasAuthorship W2891573044A5000280826 @default.
• W2891573044 hasAuthorship W2891573044A5002492478 @default.
• W2891573044 hasAuthorship W2891573044A5051244336 @default.
• W2891573044 hasAuthorship W2891573044A5058417247 @default.
• W2891573044 hasAuthorship W2891573044A5063841588 @default.
• W2891573044 hasBestOaLocation W28915730441 @default.
• W2891573044 hasConcept C104317684 @default.
• W2891573044 hasConcept C17966761 @default.
• W2891573044 hasConcept C193252679 @default.
• W2891573044 hasConcept C2777677444 @default.
• W2891573044 hasConcept C44465124 @default.
• W2891573044 hasConcept C523546767 @default.
• W2891573044 hasConcept C54355233 @default.
• W2891573044 hasConcept C86803240 @default.
• W2891573044 hasConcept C90856448 @default.
• W2891573044 hasConceptScore W2891573044C104317684 @default.
• W2891573044 hasConceptScore W2891573044C17966761 @default.
• W2891573044 hasConceptScore W2891573044C193252679 @default.
• W2891573044 hasConceptScore W2891573044C2777677444 @default.
• W2891573044 hasConceptScore W2891573044C44465124 @default.
• W2891573044 hasConceptScore W2891573044C523546767 @default.
• W2891573044 hasConceptScore W2891573044C54355233 @default.
• W2891573044 hasConceptScore W2891573044C86803240 @default.
• W2891573044 hasConceptScore W2891573044C90856448 @default.
• W2891573044 hasLocation W28915730441 @default.
• W2891573044 hasLocation W28915730442 @default.
• W2891573044 hasOpenAccess W2891573044 @default.
• W2891573044 hasPrimaryLocation W28915730441 @default.
• W2891573044 hasRelatedWork W1846728357 @default.
• W2891573044 hasRelatedWork W1996489389 @default.
• W2891573044 hasRelatedWork W2053755514 @default.
• W2891573044 hasRelatedWork W2057811770 @default.
• W2891573044 hasRelatedWork W2102210144 @default.
• W2891573044 hasRelatedWork W2110162847 @default.
• W2891573044 hasRelatedWork W2118177226 @default.
• W2891573044 hasRelatedWork W2127298790 @default.
• W2891573044 hasRelatedWork W2182757181 @default.
• W2891573044 hasRelatedWork W2350619100 @default.
• W2891573044 hasRelatedWork W2405871281 @default.
• W2891573044 hasRelatedWork W2480071061 @default.

• next