Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891619499> ?p ?o ?g. }
- W2891619499 abstract "Candida phangngensis is an ascomycetous yeast and a phylogenetic relative of the industrial workhorse Yarrowia lipolytica. Here, we report that genetic tools already established for use in the latter organism-including promoters, expression vectors, antibiotic resistance genes, a transformation protocol, and the Cre/lox system for marker recycle-can be transferred to the newer member of the Yarrowia clade with little or no need for modifications. Using these tools, we engineered C. phangngensis for improved cellulosic lipid production by introducing two heterologous yeast genes. First, overexpression of Saccharomyces cerevisiae ADH6 enhanced in situ detoxification of aldehyde fermentation inhibitors that are generated during biomass pretreatment (e.g. furfural). Subsequently, Y. lipolytica DGA1 expression boosted lipid accumulation in C. phangngensis by pulling additional carbon flux into the triacylglycerol synthesis pathway. In acid-pretreated switchgrass hydrolysate cultures, the final engineered strain JQCP04 showed a 58% decrease in lag time and a 32% increase in lipid titer as compared to wild-type PT1-17. Furthermore, we expect that this study will generate new interest in the highly oleaginous yeast C. phangngensis, which is closely related to a safe, industrial species, and is shown here to be quite amenable for genetic manipulation." @default.
- W2891619499 created "2018-09-27" @default.
- W2891619499 creator A5002311653 @default.
- W2891619499 creator A5003076901 @default.
- W2891619499 creator A5028552755 @default.
- W2891619499 creator A5073579619 @default.
- W2891619499 date "2018-09-21" @default.
- W2891619499 modified "2023-09-26" @default.
- W2891619499 title "Engineering Candida phangngensis—an oleaginous yeast from the Yarrowia clade—for enhanced detoxification of lignocellulose-derived inhibitors and lipid overproduction" @default.
- W2891619499 cites W1850569493 @default.
- W2891619499 cites W1969336035 @default.
- W2891619499 cites W1971439989 @default.
- W2891619499 cites W1975909230 @default.
- W2891619499 cites W1982183605 @default.
- W2891619499 cites W1983188063 @default.
- W2891619499 cites W1988079922 @default.
- W2891619499 cites W1990161207 @default.
- W2891619499 cites W2011893021 @default.
- W2891619499 cites W2012746361 @default.
- W2891619499 cites W2014390233 @default.
- W2891619499 cites W2018111919 @default.
- W2891619499 cites W2018214673 @default.
- W2891619499 cites W2035681245 @default.
- W2891619499 cites W2038812526 @default.
- W2891619499 cites W2040981627 @default.
- W2891619499 cites W2049965804 @default.
- W2891619499 cites W2056077497 @default.
- W2891619499 cites W2061084055 @default.
- W2891619499 cites W2068402580 @default.
- W2891619499 cites W2072261755 @default.
- W2891619499 cites W2074055936 @default.
- W2891619499 cites W2074072123 @default.
- W2891619499 cites W2075918459 @default.
- W2891619499 cites W2075961058 @default.
- W2891619499 cites W2076830784 @default.
- W2891619499 cites W2086458980 @default.
- W2891619499 cites W2096454544 @default.
- W2891619499 cites W2106935141 @default.
- W2891619499 cites W2107910846 @default.
- W2891619499 cites W2116775961 @default.
- W2891619499 cites W2119779443 @default.
- W2891619499 cites W2131183871 @default.
- W2891619499 cites W2149460148 @default.
- W2891619499 cites W2166298490 @default.
- W2891619499 cites W2169832544 @default.
- W2891619499 cites W2277604014 @default.
- W2891619499 cites W2292710945 @default.
- W2891619499 cites W2313226053 @default.
- W2891619499 cites W2329815048 @default.
- W2891619499 cites W2344071659 @default.
- W2891619499 cites W2462787491 @default.
- W2891619499 cites W2474896427 @default.
- W2891619499 cites W2496855910 @default.
- W2891619499 cites W2500245354 @default.
- W2891619499 cites W2513079084 @default.
- W2891619499 cites W2513434026 @default.
- W2891619499 cites W2563320377 @default.
- W2891619499 cites W2565808669 @default.
- W2891619499 cites W2572443119 @default.
- W2891619499 cites W2767637257 @default.
- W2891619499 cites W2793192195 @default.
- W2891619499 cites W4211035205 @default.
- W2891619499 cites W4240212335 @default.
- W2891619499 doi "https://doi.org/10.1093/femsyr/foy102" @default.
- W2891619499 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30247683" @default.
- W2891619499 hasPublicationYear "2018" @default.
- W2891619499 type Work @default.
- W2891619499 sameAs 2891619499 @default.
- W2891619499 citedByCount "19" @default.
- W2891619499 countsByYear W28916194992020 @default.
- W2891619499 countsByYear W28916194992021 @default.
- W2891619499 countsByYear W28916194992022 @default.
- W2891619499 countsByYear W28916194992023 @default.
- W2891619499 crossrefType "journal-article" @default.
- W2891619499 hasAuthorship W2891619499A5002311653 @default.
- W2891619499 hasAuthorship W2891619499A5003076901 @default.
- W2891619499 hasAuthorship W2891619499A5028552755 @default.
- W2891619499 hasAuthorship W2891619499A5073579619 @default.
- W2891619499 hasBestOaLocation W28916194991 @default.
- W2891619499 hasConcept C100544194 @default.
- W2891619499 hasConcept C104317684 @default.
- W2891619499 hasConcept C124420848 @default.
- W2891619499 hasConcept C2777576037 @default.
- W2891619499 hasConcept C2779039970 @default.
- W2891619499 hasConcept C2779222958 @default.
- W2891619499 hasConcept C2779490540 @default.
- W2891619499 hasConcept C2780115692 @default.
- W2891619499 hasConcept C55493867 @default.
- W2891619499 hasConcept C6350086 @default.
- W2891619499 hasConcept C86803240 @default.
- W2891619499 hasConcept C89423630 @default.
- W2891619499 hasConceptScore W2891619499C100544194 @default.
- W2891619499 hasConceptScore W2891619499C104317684 @default.
- W2891619499 hasConceptScore W2891619499C124420848 @default.
- W2891619499 hasConceptScore W2891619499C2777576037 @default.
- W2891619499 hasConceptScore W2891619499C2779039970 @default.
- W2891619499 hasConceptScore W2891619499C2779222958 @default.
- W2891619499 hasConceptScore W2891619499C2779490540 @default.
- W2891619499 hasConceptScore W2891619499C2780115692 @default.
- W2891619499 hasConceptScore W2891619499C55493867 @default.