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- W2891620034 abstract "Protocols were successfully established for in vitro regeneration and coloured callusproduction of Echinocereus cinerascens. Investigation mainly focused on the effects ofplant growth regulators in rapid production of this endangered species and the optimumproduction of coloured callus. Additional assessments studied concerning theproduction of synthetic seeds, extraction of natural pigments and detection ofsomaclonal variation of the regenerants. Rapid production through direct in vitroregeneration gave the highest mean number of shoots, 4.37 ± 0.27, observed in MSmedium supplemented with 2.0 mg/l Kinetin + 1.0 mg/l IBA which promoted thehighest production of shoots after 4 months, 131 shoots in total. Nevertheless, throughindirect in vitro regeneration, somatic embryos of Echinocereus cinerascens weresuccessfully developed in two treatments of liquid medium including MS mediumsupplemented with 0.5 mg/l 2,4-D + 0.1 mg/l BAP + 0.5 mg/l thiamine HCl and MSmedium supplemented with 0.5 mg/l 2,4-D + 0.5 mg/l BAP + 0.5 mg/l thiamine HCl, asboth media promoted 100% total mean production of somatic embryos (globular, heart,torpedo-shaped and cotyledonary stage) after 4 months. Practically, the production ofideal synthetic seeds was successfully established whereby, micro shoots as the mostresponsive propagule were encapsulated in 3% of sodium alginate hardened in 100 mMof calcium chloride dehydrate solution for 30 minutes gave 100% of germination rateafter 4 months. Complete plantlets were successfully acclimatized with the highestsurvival rate of 90% observed in sand, the most suitable planting substrate whichpossessed 3 major elements such as SiO2, Al2O3 and CaO that play important roles tosupport the growth of Echinocereus cinerascens. Basically, the present study indicatedthat 100% production of green, yellow and pink callus was obtained after 2 months inseveral treatments of MS medium supplemented with 2,4-D + BAP + thiamine HClapplied in combination. Interestingly, the occurrence of dramatic changes in the production of coloured callus was clearly observed in a conversion of green to pinkcallus within 2 months. Moreover, pigment extraction analysis through UV-VISspectroscopy discovered that both regenerants and callus possessed chlorophyll a and bas the major pigment while carotenoids as the minor pigment. Meanwhile, HPLCanalysis revealed individual carotenoids present in in vitro plantlets namely, neoxanthin,β-carotene, lutein and violaxanthin, whereas in callus were β-carotene and lutein only.Analysis of cytological studies clarified that there were no significant differences in cellorganization and behaviour of in vitro plantlets and ex vitro plants. The evidenceverified that Echinocereus cinerascens regenerated normally in vitro and grownvigorously after being transferred to the natural environment" @default.
- W2891620034 created "2018-09-27" @default.
- W2891620034 creator A5045947739 @default.
- W2891620034 date "2017-08-01" @default.
- W2891620034 modified "2023-09-27" @default.
- W2891620034 title "Micropropagation and pigment extraction of Echinocereus cinerascens / Hashimah Elias" @default.
- W2891620034 hasPublicationYear "2017" @default.
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