FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891623539> ?p ?o ?g. }

• W2891623539 abstract "Abstract High throughput sequencing has been proposed as a one-stop solution for diagnostics and molecular typing directly from patient samples, allowing timely and appropriate implementation of measures for treatment, infection prevention and control. However, it is unclear how the variety of available methods impacts the end results. We applied shotgun metagenomics on diverse types of patient samples using three different methods to deplete human DNA prior to DNA extraction. Libraries were prepared and sequenced with Illumina chemistry. Data was analyzed using methods likely to be available in clinical microbiology laboratories using genomics. The results of microbial identification were compared to standard culture-based microbiological methods. On average, 75% of the reads corresponded to human DNA, being a major determinant in the analysis outcome. None of the kits was clearly superior suggesting that the initial ratio between host and microbial DNA or other sample characteristics were the major determinants of the proportion of microbial reads. Most pathogens identified by culture were also identified through metagenomics, but substantial differences were noted between the taxonomic classification tools. In two cases the high number of human reads resulted in insufficient sequencing depth of bacterial DNA for identification. In three samples, we could infer the probable multilocus sequence type of the most abundant species. The tools and databases used for taxonomic classification and antimicrobial resistance identification had a key impact on the results, recommending that efforts need to be aimed at standardization of the analysis methods if metagenomics is to be used routinely in clinical microbiology." @default.
• W2891623539 created "2018-09-27" @default.
• W2891623539 creator A5008289066 @default.
• W2891623539 creator A5017303734 @default.
• W2891623539 creator A5026819464 @default.
• W2891623539 creator A5029356969 @default.
• W2891623539 creator A5033018668 @default.
• W2891623539 creator A5039666775 @default.
• W2891623539 creator A5044514487 @default.
• W2891623539 creator A5045293362 @default.
• W2891623539 creator A5048075811 @default.
• W2891623539 creator A5052955378 @default.
• W2891623539 creator A5057086640 @default.
• W2891623539 creator A5075796846 @default.
• W2891623539 creator A5082344930 @default.
• W2891623539 creator A5083910434 @default.
• W2891623539 date "2018-09-13" @default.
• W2891623539 modified "2023-10-18" @default.
• W2891623539 title "Critical steps in clinical shotgun metagenomics for the concomitant detection and typing of microbial pathogens" @default.
• W2891623539 cites W1806778326 @default.
• W2891623539 cites W1828084056 @default.
• W2891623539 cites W1829807246 @default.
• W2891623539 cites W2051248332 @default.
• W2891623539 cites W2108234281 @default.
• W2891623539 cites W2120902911 @default.
• W2891623539 cites W2121438595 @default.
• W2891623539 cites W2131026689 @default.
• W2891623539 cites W2141041418 @default.
• W2891623539 cites W2154003902 @default.
• W2891623539 cites W2159954944 @default.
• W2891623539 cites W2170551349 @default.
• W2891623539 cites W2308191534 @default.
• W2891623539 cites W2332382218 @default.
• W2891623539 cites W2337752917 @default.
• W2891623539 cites W2403288892 @default.
• W2891623539 cites W2523445937 @default.
• W2891623539 cites W2533132894 @default.
• W2891623539 cites W2561512876 @default.
• W2891623539 cites W2583965592 @default.
• W2891623539 cites W2587565773 @default.
• W2891623539 cites W2607011121 @default.
• W2891623539 cites W2624010287 @default.
• W2891623539 cites W2739323458 @default.
• W2891623539 cites W2766658767 @default.
• W2891623539 doi "https://doi.org/10.1038/s41598-018-31873-w" @default.
• W2891623539 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6137123" @default.
• W2891623539 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30213965" @default.
• W2891623539 hasPublicationYear "2018" @default.
• W2891623539 type Work @default.
• W2891623539 sameAs 2891623539 @default.
• W2891623539 citedByCount "47" @default.
• W2891623539 countsByYear W28916235392019 @default.
• W2891623539 countsByYear W28916235392020 @default.
• W2891623539 countsByYear W28916235392021 @default.
• W2891623539 countsByYear W28916235392022 @default.
• W2891623539 countsByYear W28916235392023 @default.
• W2891623539 crossrefType "journal-article" @default.
• W2891623539 hasAuthorship W2891623539A5008289066 @default.
• W2891623539 hasAuthorship W2891623539A5017303734 @default.
• W2891623539 hasAuthorship W2891623539A5026819464 @default.
• W2891623539 hasAuthorship W2891623539A5029356969 @default.
• W2891623539 hasAuthorship W2891623539A5033018668 @default.
• W2891623539 hasAuthorship W2891623539A5039666775 @default.
• W2891623539 hasAuthorship W2891623539A5044514487 @default.
• W2891623539 hasAuthorship W2891623539A5045293362 @default.
• W2891623539 hasAuthorship W2891623539A5048075811 @default.
• W2891623539 hasAuthorship W2891623539A5052955378 @default.
• W2891623539 hasAuthorship W2891623539A5057086640 @default.
• W2891623539 hasAuthorship W2891623539A5075796846 @default.
• W2891623539 hasAuthorship W2891623539A5082344930 @default.
• W2891623539 hasAuthorship W2891623539A5083910434 @default.
• W2891623539 hasBestOaLocation W28916235391 @default.
• W2891623539 hasConcept C101985253 @default.
• W2891623539 hasConcept C104317684 @default.
• W2891623539 hasConcept C116834253 @default.
• W2891623539 hasConcept C135763542 @default.
• W2891623539 hasConcept C141231307 @default.
• W2891623539 hasConcept C15151743 @default.
• W2891623539 hasConcept C18903297 @default.
• W2891623539 hasConcept C189206191 @default.
• W2891623539 hasConcept C2781209916 @default.
• W2891623539 hasConcept C2781434637 @default.
• W2891623539 hasConcept C2992965680 @default.
• W2891623539 hasConcept C49105822 @default.
• W2891623539 hasConcept C51679486 @default.
• W2891623539 hasConcept C54355233 @default.
• W2891623539 hasConcept C552990157 @default.
• W2891623539 hasConcept C70721500 @default.
• W2891623539 hasConcept C77434933 @default.
• W2891623539 hasConcept C78063203 @default.
• W2891623539 hasConcept C86803240 @default.
• W2891623539 hasConcept C89423630 @default.
• W2891623539 hasConceptScore W2891623539C101985253 @default.
• W2891623539 hasConceptScore W2891623539C104317684 @default.
• W2891623539 hasConceptScore W2891623539C116834253 @default.
• W2891623539 hasConceptScore W2891623539C135763542 @default.
• W2891623539 hasConceptScore W2891623539C141231307 @default.
• W2891623539 hasConceptScore W2891623539C15151743 @default.
• W2891623539 hasConceptScore W2891623539C18903297 @default.
• W2891623539 hasConceptScore W2891623539C189206191 @default.

• next