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• W2891628998 abstract "Bacterial multidrug transporter DrrAB exhibits overlapping substrate specificity with mammalian P-glycoprotein. DrrA hydrolyzes ATP, and the energy is transduced to carrier DrrB resulting in export of drugs. Previous studies suggested that DrrB contains a large and flexible drug-binding pocket made of aromatic residues contributed by several transmembrane helices with different drugs binding to both specific and shared residues in this pocket. However, direct binding of drugs to DrrAB or the mechanism of substrate-induced conformational changes between DrrA and DrrB has so far not been investigated. We used two fluorescence-based approaches to determine substrate binding to purified DrrAB. Our analysis shows that DrrB binds drugs with variable affinities and contains multiple drug binding sites. This work also provides evidence for two asymmetric nucleotide binding sites in DrrA with strikingly different binding affinities. Using targeted fluorescence labeling, we provide clear evidence of long-range conformational changes occurring between DrrA and DrrB. It is proposed that the transduction pathway from the nucleotide-binding DrrA subunit to the substrate binding DrrB subunit includes Q-loop and CREEM motifs in DrrA and EAA-like motif in DrrB. This study lays a solid groundwork for examining roles of various conserved regions of DrrA and DrrB in transduction of conformational changes." @default.
• W2891628998 created "2018-09-27" @default.
• W2891628998 creator A5002535999 @default.
• W2891628998 creator A5013311070 @default.
• W2891628998 date "2018-11-01" @default.
• W2891628998 modified "2023-10-16" @default.
• W2891628998 title "Conformational changes in a multidrug resistance ABC transporter DrrAB: Fluorescence-based approaches to study substrate binding" @default.
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• W2891628998 doi "https://doi.org/10.1016/j.abb.2018.09.017" @default.
• W2891628998 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6365169" @default.
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• W2891628998 hasPublicationYear "2018" @default.
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