Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891635336> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2891635336 endingPage "369" @default.
- W2891635336 startingPage "369" @default.
- W2891635336 abstract "Background: Mangrove plants have high potential in medical research and they have been used in traditional medicine for any diseases. Squalene, a natural 30-carbon triterpenoid compound, was originally obtained from Shark and for the first time isolated from mangrove plant species. From the historical background, it was known that squalene display protective actions against several diseases and carcinogens. Objective: Herein, we desire to report the in vitro evaluation of squalene on AGS cell line. In the present investigation, after 24 h incubation, the inhibitory effect of squalene was found to have significant activity on AGS cell lines proliferation. Materials and Methods: Mangrove plant isolated squalene compound was treated with gastric adenocarcinoma cancer cell line AGS for 24 h with control. The cells were treated at varying concentration range 10, 30, and 50 μg/mL. The cytotoxicity effect of squalene was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide, mitochondria membrane potential, etc., Results: Among the tested concentrations, squalene at 50 μg/mL showed a higher level of mitochondrial depolarization, DNA fragmentation, and induced apoptosis in AGS cells when compared to control. A similar but lower activity was observed in minimum doses of 20 and 30 μg/mL compared to maximum dose of 50 μg/mL. A 50% and 100% cell viability was observed at 30 and 50 μg/mL, respectively. Likewise, there was a significant reduction in thiobarbituric acid reactive substrate and lipid hydroperoxides levels, while antioxidant enzymes like superoxide dismutase. Catalase, glutathione (GSH) peroxidase, reduced GSH, Vitamin-C (Vit-C), and Vitamin-E were increased on squalene treatment in a dose-dependent manner. Conclusion: On the basis of results, it was concluded that isolated compound (squalene) from Rhizophora mucronata had a potent antigastric carcinogenic effect at 50 μg/mL in AGS cell lines and appeared to be more sensitive toward the AGS cell line. Abbreviations used: PBS: Phosphate-buffered saline; FBS: Fetal bovine serum; DMEM: Dulbecco's modified eagle medium; MTT: 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-tetrazolium bromide; DCFH-DH-7: Diacetyl dichlorofluorescein; Rh 123: Rhodamine 123; EB: Ethidium bromide; AO: Acridine orange; TBA: Thiobarbituric acid; NBT: Nitro blue tetrazolium; PMS: Phenazine methosulfate; DTNB-5: 5-dithiobis 2-nitrobenzoic acid; GSH: Reduced glutathione." @default.
- W2891635336 created "2018-09-27" @default.
- W2891635336 creator A5000543814 @default.
- W2891635336 creator A5006997460 @default.
- W2891635336 creator A5007109382 @default.
- W2891635336 creator A5047049313 @default.
- W2891635336 creator A5056569327 @default.
- W2891635336 date "2018-01-01" @default.
- W2891635336 modified "2023-10-12" @default.
- W2891635336 title "In Vitro antigastric cancer activity of squalene, a triterpenoid compound isolated from Rhizophora Mucronata mangrove plant leaves against AGS cell line" @default.
- W2891635336 cites W1974061717 @default.
- W2891635336 doi "https://doi.org/10.4103/pm.pm_577_17" @default.
- W2891635336 hasPublicationYear "2018" @default.
- W2891635336 type Work @default.
- W2891635336 sameAs 2891635336 @default.
- W2891635336 citedByCount "12" @default.
- W2891635336 countsByYear W28916353362019 @default.
- W2891635336 countsByYear W28916353362020 @default.
- W2891635336 countsByYear W28916353362021 @default.
- W2891635336 countsByYear W28916353362023 @default.
- W2891635336 crossrefType "journal-article" @default.
- W2891635336 hasAuthorship W2891635336A5000543814 @default.
- W2891635336 hasAuthorship W2891635336A5006997460 @default.
- W2891635336 hasAuthorship W2891635336A5007109382 @default.
- W2891635336 hasAuthorship W2891635336A5047049313 @default.
- W2891635336 hasAuthorship W2891635336A5056569327 @default.
- W2891635336 hasConcept C107585567 @default.
- W2891635336 hasConcept C121608353 @default.
- W2891635336 hasConcept C185592680 @default.
- W2891635336 hasConcept C18903297 @default.
- W2891635336 hasConcept C193950572 @default.
- W2891635336 hasConcept C202751555 @default.
- W2891635336 hasConcept C2776703722 @default.
- W2891635336 hasConcept C2777817122 @default.
- W2891635336 hasConcept C2777912774 @default.
- W2891635336 hasConcept C2778538235 @default.
- W2891635336 hasConcept C2994372470 @default.
- W2891635336 hasConcept C54355233 @default.
- W2891635336 hasConcept C55493867 @default.
- W2891635336 hasConcept C556039675 @default.
- W2891635336 hasConcept C59822182 @default.
- W2891635336 hasConcept C68874143 @default.
- W2891635336 hasConcept C71924100 @default.
- W2891635336 hasConcept C86803240 @default.
- W2891635336 hasConcept C96232424 @default.
- W2891635336 hasConceptScore W2891635336C107585567 @default.
- W2891635336 hasConceptScore W2891635336C121608353 @default.
- W2891635336 hasConceptScore W2891635336C185592680 @default.
- W2891635336 hasConceptScore W2891635336C18903297 @default.
- W2891635336 hasConceptScore W2891635336C193950572 @default.
- W2891635336 hasConceptScore W2891635336C202751555 @default.
- W2891635336 hasConceptScore W2891635336C2776703722 @default.
- W2891635336 hasConceptScore W2891635336C2777817122 @default.
- W2891635336 hasConceptScore W2891635336C2777912774 @default.
- W2891635336 hasConceptScore W2891635336C2778538235 @default.
- W2891635336 hasConceptScore W2891635336C2994372470 @default.
- W2891635336 hasConceptScore W2891635336C54355233 @default.
- W2891635336 hasConceptScore W2891635336C55493867 @default.
- W2891635336 hasConceptScore W2891635336C556039675 @default.
- W2891635336 hasConceptScore W2891635336C59822182 @default.
- W2891635336 hasConceptScore W2891635336C68874143 @default.
- W2891635336 hasConceptScore W2891635336C71924100 @default.
- W2891635336 hasConceptScore W2891635336C86803240 @default.
- W2891635336 hasConceptScore W2891635336C96232424 @default.
- W2891635336 hasIssue "57" @default.
- W2891635336 hasLocation W28916353361 @default.
- W2891635336 hasOpenAccess W2891635336 @default.
- W2891635336 hasPrimaryLocation W28916353361 @default.
- W2891635336 hasRelatedWork W1567515804 @default.
- W2891635336 hasRelatedWork W2040454192 @default.
- W2891635336 hasRelatedWork W2106771900 @default.
- W2891635336 hasRelatedWork W2130774518 @default.
- W2891635336 hasRelatedWork W2187828558 @default.
- W2891635336 hasRelatedWork W2526513837 @default.
- W2891635336 hasRelatedWork W2891635336 @default.
- W2891635336 hasRelatedWork W2971956568 @default.
- W2891635336 hasRelatedWork W3211511967 @default.
- W2891635336 hasRelatedWork W4387309048 @default.
- W2891635336 hasVolume "14" @default.
- W2891635336 isParatext "false" @default.
- W2891635336 isRetracted "false" @default.
- W2891635336 magId "2891635336" @default.
- W2891635336 workType "article" @default.