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• W2891639002 abstract "Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose.A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-GFP), as a reporter gene, was amplified by PCR and then sub-cloned in the pHEN4/3VGR19 phagemid. The resulting construct was transfected into 293KDR cell. The recombinant phage was extracted and confirmed and then transduced into VEGFR2 expressing cell (293KDR).Seventy-two hr after transfection, green fluorescence was detected in 30% of the cells. About 1% of the cells which transduced by recombinant phages were able to express GFP.It is hoped that the results from this study will help to find potential vectors to improve the efficiency of gene delivery. Taken together, we conclude that this newly-introduced vector can be used in cancer researches." @default.
• W2891639002 created "2018-09-27" @default.
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• W2891639002 date "2018-09-01" @default.
• W2891639002 modified "2023-09-26" @default.
• W2891639002 title "Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody." @default.
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• W2891639002 doi "https://doi.org/10.22038/ijbms.2018.26191.6432" @default.
• W2891639002 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6272075" @default.
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