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• W2891639386 endingPage "307" @default.
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• W2891639386 abstract "Summary The aim of this study was to establish a functional freezing–thawing protocol for epididymal sperm of collared peccaries ( Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed. In addition, to evaluate the survival of frozen–thawed sperm, a thermal resistance test (TRT) was executed. Samples preserved using Tris were in better condition compared with those preserved using ACP ® , showing higher values for most assessments performed, including CASA and the TRT ( P <0.05). After determining Tris to be the better of the two extenders, additional samples were thawed using different thawing rates (37°C/60 s, 55°C/7 s, 70°C/8 s). Sperm thawed at 37°C/60 s had the greatest preservation ( P <0.05) of viability (54.1 ± 5.9%) and functional membrane integrity (43.2 ± 5.4%), and had higher values for various CASA parameters. In conclusion, we suggest the use of a Tris-based extender added to egg yolk and glycerol for the cryopreservation of epididymal sperm obtained from collared peccaries. In order to achieve better post-thawing sperm quality, we suggest that samples should be thawed at 37°C/60 s." @default.
• W2891639386 created "2018-09-27" @default.
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• W2891639386 date "2018-08-01" @default.
• W2891639386 modified "2023-09-23" @default.
• W2891639386 title "Cryopreservation of collared peccary (<i>Pecari tajacu</i> L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)" @default.
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• W2891639386 doi "https://doi.org/10.1017/s0967199418000230" @default.
• W2891639386 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30220256" @default.
• W2891639386 hasPublicationYear "2018" @default.
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