Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891645373> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2891645373 endingPage "65" @default.
- W2891645373 startingPage "59" @default.
- W2891645373 abstract "In recent years, there has been an increase in reported cases of fowl adenovirus serotype 4 (FAdV-4) in chickens in China. The use of live attenuated vaccines contaminated with FAdV-4 has been proved to be one of the important causes of massive outbreaks of hydropericardium syndrome. To detect the contamination with FAdV-4 in attenuated vaccines more promptly and accurately, a droplet digital PCR (ddPCR) assay was developed for the rapid detection of FAdV-4 and FAdV-10. The ability of this assay to detect FAdV-4 contamination in attenuated Newcastle disease virus vaccines was assessed in comparison to a quantitative real-time PCR (qPCR) and a conventional PCR assay. The findings indicated that the ddPCR assay could detect FAdV-4 contamination at 0.1 EID50/1,000 feathers, while the qPCR could detect FAdV-4 contamination at 1 EID50/1,000 feathers with identical genomic targets, which was 1,000-fold more sensitive than conventional PCR detection with a sensitivity of 102 EID50/1,000 feathers. The ddPCR assay also showed high specificity for FAdV-4/10 and no positive signals were detected for other FAdVs. Consequently, the intuitive and rapid results were especially suitable for the detection of FAdV-4 contamination in vaccines. In this study, a ddPCR assay was developed to effectively detect and quantify low-dose FAdV-4 contamination, providing a new method for rapid detection of FAdV-4 contamination in various samples, especially vaccines." @default.
- W2891645373 created "2018-09-27" @default.
- W2891645373 creator A5000141316 @default.
- W2891645373 creator A5000793847 @default.
- W2891645373 creator A5002849906 @default.
- W2891645373 creator A5022590540 @default.
- W2891645373 creator A5064919743 @default.
- W2891645373 creator A5076622154 @default.
- W2891645373 creator A5087791435 @default.
- W2891645373 date "2019-03-01" @default.
- W2891645373 modified "2023-10-16" @default.
- W2891645373 title "Development and evaluation of a droplet digital PCR assay for the detection of fowl adenovirus serotypes 4 and 10 in attenuated vaccines" @default.
- W2891645373 cites W1965535093 @default.
- W2891645373 cites W2056642329 @default.
- W2891645373 cites W2064543601 @default.
- W2891645373 cites W2087350640 @default.
- W2891645373 cites W2091060176 @default.
- W2891645373 cites W2099340333 @default.
- W2891645373 cites W2128136927 @default.
- W2891645373 cites W2138970503 @default.
- W2891645373 cites W2147994903 @default.
- W2891645373 cites W2175782529 @default.
- W2891645373 cites W2476262018 @default.
- W2891645373 cites W2508116434 @default.
- W2891645373 cites W2545217437 @default.
- W2891645373 cites W2547087767 @default.
- W2891645373 cites W2551889460 @default.
- W2891645373 cites W2624852724 @default.
- W2891645373 cites W2792232091 @default.
- W2891645373 doi "https://doi.org/10.1016/j.jviromet.2018.09.005" @default.
- W2891645373 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30222990" @default.
- W2891645373 hasPublicationYear "2019" @default.
- W2891645373 type Work @default.
- W2891645373 sameAs 2891645373 @default.
- W2891645373 citedByCount "10" @default.
- W2891645373 countsByYear W28916453732019 @default.
- W2891645373 countsByYear W28916453732020 @default.
- W2891645373 countsByYear W28916453732021 @default.
- W2891645373 countsByYear W28916453732022 @default.
- W2891645373 countsByYear W28916453732023 @default.
- W2891645373 crossrefType "journal-article" @default.
- W2891645373 hasAuthorship W2891645373A5000141316 @default.
- W2891645373 hasAuthorship W2891645373A5000793847 @default.
- W2891645373 hasAuthorship W2891645373A5002849906 @default.
- W2891645373 hasAuthorship W2891645373A5022590540 @default.
- W2891645373 hasAuthorship W2891645373A5064919743 @default.
- W2891645373 hasAuthorship W2891645373A5076622154 @default.
- W2891645373 hasAuthorship W2891645373A5087791435 @default.
- W2891645373 hasConcept C10389963 @default.
- W2891645373 hasConcept C104317684 @default.
- W2891645373 hasConcept C112570922 @default.
- W2891645373 hasConcept C127745971 @default.
- W2891645373 hasConcept C159047783 @default.
- W2891645373 hasConcept C18903297 @default.
- W2891645373 hasConcept C2522874641 @default.
- W2891645373 hasConcept C2778116459 @default.
- W2891645373 hasConcept C48023723 @default.
- W2891645373 hasConcept C48126324 @default.
- W2891645373 hasConcept C49105822 @default.
- W2891645373 hasConcept C54355233 @default.
- W2891645373 hasConcept C86803240 @default.
- W2891645373 hasConceptScore W2891645373C10389963 @default.
- W2891645373 hasConceptScore W2891645373C104317684 @default.
- W2891645373 hasConceptScore W2891645373C112570922 @default.
- W2891645373 hasConceptScore W2891645373C127745971 @default.
- W2891645373 hasConceptScore W2891645373C159047783 @default.
- W2891645373 hasConceptScore W2891645373C18903297 @default.
- W2891645373 hasConceptScore W2891645373C2522874641 @default.
- W2891645373 hasConceptScore W2891645373C2778116459 @default.
- W2891645373 hasConceptScore W2891645373C48023723 @default.
- W2891645373 hasConceptScore W2891645373C48126324 @default.
- W2891645373 hasConceptScore W2891645373C49105822 @default.
- W2891645373 hasConceptScore W2891645373C54355233 @default.
- W2891645373 hasConceptScore W2891645373C86803240 @default.
- W2891645373 hasFunder F4320335777 @default.
- W2891645373 hasLocation W28916453731 @default.
- W2891645373 hasLocation W28916453732 @default.
- W2891645373 hasOpenAccess W2891645373 @default.
- W2891645373 hasPrimaryLocation W28916453731 @default.
- W2891645373 hasRelatedWork W147206206 @default.
- W2891645373 hasRelatedWork W1968969368 @default.
- W2891645373 hasRelatedWork W1972497539 @default.
- W2891645373 hasRelatedWork W2044607317 @default.
- W2891645373 hasRelatedWork W2124927935 @default.
- W2891645373 hasRelatedWork W2334200357 @default.
- W2891645373 hasRelatedWork W2392929227 @default.
- W2891645373 hasRelatedWork W3036646312 @default.
- W2891645373 hasRelatedWork W4240867133 @default.
- W2891645373 hasRelatedWork W4281608676 @default.
- W2891645373 hasVolume "265" @default.
- W2891645373 isParatext "false" @default.
- W2891645373 isRetracted "false" @default.
- W2891645373 magId "2891645373" @default.
- W2891645373 workType "article" @default.