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- W2891649283 abstract "e20004 Background: Increasing evidence illustrates that exosomal microRNAs in circulating fluids provide a promising way as biomarkers for noninvasive cancer diagnosis. The aim of this study was to evaluate the feasibility of exosomal miRNAs as a diagnostic and prognostic biomarkers in non-small-cell lung cancer (NSCLC). Methods: Blood samples from 40 NSCLC patients and 24 healthy volunteers were collected and matched with age, gender and blood collection time. Plasma exosomes were accessed by 110,000×g ultracentrifugation and visualized by NS300 equipment. The raw data of exosomal miRNA profiles of NSCLC patients and healthy individuals were generated by NGS around 400× read depth and its expression were measured by Taqman probe quantitive PCR. . Results: Exosome numbers were robust in differentiating NSCLC patients from controls [area under the curve (AUC) = 0.800; 95% confidence interval (CI) = 0.685–0.915 P < 0.01]. RNA sequencing data showed 31 miRNAs were up-regulated and 55 miRNAs down-regulated in plasma of NSCLC. TaqMan quantitative RT-PCR analyses indicated that miR-99a, miR-124 and miR-214 were significantly lower in NSCLC patients than in controls. However, miR-21 and miR-126 had significantly higher expression in NSCLC patients. Mir-214 showed high accuracy in discriminating NSCLC patients from healthy controls with a sensitivity of 0.70 and specify of 0.92 (AUC = 0.858, 95% CI = 0.765–0.950 P < 0.001). Furthermore, decreased miR-99a expression was positively correlated with TNM stage (P = 0.007), lymph node metastasis (P = 0.012), and distant metastasis (P < 0.001). Increased miR-21 expression was significantly associated with poorer pathological differentiation (P = 0.015), tumor size (P = 0.033), and lymph node metastasis (P = 0.028). Kaplan–Meier analysis demonstrated that decreased miR-99a expression contributed to poor progression-free survival (PFS) (P < 0.01) of patients. Multivariate analysis indicated that miR-99a was a independent prognostic marker of survival. Conclusions: Exosomal miRNAs in plasma could indicate the progress of NSCLC and a combination of the explored miRNA could serve as a promising biomarker for NSCLS diagnosis and prognosis." @default.
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- W2891649283 date "2016-05-20" @default.
- W2891649283 modified "2023-09-27" @default.
- W2891649283 title "Evaluation of exosomal miRNAs from plasma as potential biomarkers for non-small-cell lung cancer." @default.
- W2891649283 doi "https://doi.org/10.1200/jco.2016.34.15_suppl.e20004" @default.
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