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- W2891657573 abstract "Due to the scarcity of tissues and organs for transplantation, the demand for bioengineered tissues is increasing with the advancement of technologies and new treatments in human and animal regenerative medicine. Thus, decellularized placental extracellular matrix (ECM) has emerged as a new tool for the production of biological scaffolds for subsequent recellularization and implantation for recovery of injured areas or even for replacement of organ and tissue fractions. To be classified as an ideal biological scaffold, the ECM must be acellular and preserve its proteins and physical features to be useful for cellular adhesion. In this context, we developed a process of decellularization of canine placentas with 35 and 40 days of gestation using dodecyl sulfate sodium under immersion and agitation in sterile conditions. Before use of this scaffold in recellularization processes, the decellularization efficiency needs to be confirmed by the absence of cellular content and an irrelevant amount of reminiscent DNA. Both vasculature architecture and ECM proteins, such as collagen types I, III, and IV, laminin, and fibronectin, were preserved with our method. In this way, we established a new biological scaffold model that could be used for recellularization in regenerative medicine of tissues." @default.
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- W2891657573 date "2018-01-01" @default.
- W2891657573 modified "2023-10-10" @default.
- W2891657573 title "Optimization of Canine Placenta Decellularization: An Alternative Source of Biological Scaffolds for Regenerative Medicine" @default.
- W2891657573 doi "https://doi.org/10.1159/000492466" @default.
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