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- W2891674430 abstract "Abstract Dominant negative polypeptides can act as inhibitors by binding to the wild type protein or by titrating an essential ligand. Here, we use high-throughput sequencing of DNA libraries composed of fragments of yeast genes to identify dominant negative polypeptides based on their depletion during cell growth. The method uncovers numerous inhibitory polypeptides for a protein and thus is capable of defining interacting domains with exquisite resolution, even pinpointing individual residues that contact ligands." @default.
- W2891674430 created "2018-09-27" @default.
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- W2891674430 date "2018-09-15" @default.
- W2891674430 modified "2023-10-16" @default.
- W2891674430 title "High-throughput identification of dominant negative polypeptides in yeast" @default.
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- W2891674430 doi "https://doi.org/10.1101/418608" @default.
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