Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891675981> ?p ?o ?g. }
- W2891675981 abstract "Abstract Cone snails are a diverse group of predatory marine invertebrates that deploy remarkably complex venoms to rapidly paralyse worm, mollusc or fish prey. ω-Conotoxins are neurotoxic peptides from cone snail venoms that inhibit Ca v 2.2 voltage-gated calcium channel, demonstrating potential for pain management via intrathecal (IT) administration. Here, we isolated and characterized two novel ω-conotoxins, MoVIA and MoVIB from Conus moncuri , the first to be identified in vermivorous (worm-hunting) cone snails. MoVIA and MoVIB potently inhibited human Ca v 2.2 in fluorimetric assays and rat Ca v 2.2 in patch clamp studies, and both potently displaced radiolabeled ω-conotoxin GVIA ( 125 I-GVIA) from human SH-SY5Y cells and fish brain membranes (IC 50 2–9 pM). Intriguingly, an arginine at position 13 in MoVIA and MoVIB replaced the functionally critical tyrosine found in piscivorous ω-conotoxins. To investigate its role, we synthesized MoVIB-[R13Y] and MVIIA-[Y13R]. Interestingly, MVIIA-[Y13R] completely lost Ca v 2.2 activity and MoVIB-[R13Y] had reduced activity, indicating that Arg at position 13 was preferred in these vermivorous ω-conotoxins whereas tyrosine 13 is preferred in piscivorous ω-conotoxins. MoVIB reversed pain behavior in a rat neuropathic pain model, confirming that vermivorous cone snails are a new source of analgesic ω-conotoxins. Given vermivorous cone snails are ancestral to piscivorous species, our findings support the repurposing of defensive venom peptides in the evolution of piscivorous Conidae ." @default.
- W2891675981 created "2018-09-27" @default.
- W2891675981 creator A5000965397 @default.
- W2891675981 creator A5005160521 @default.
- W2891675981 creator A5025386235 @default.
- W2891675981 creator A5025539645 @default.
- W2891675981 creator A5032618006 @default.
- W2891675981 creator A5032924771 @default.
- W2891675981 creator A5033658079 @default.
- W2891675981 creator A5042555323 @default.
- W2891675981 creator A5058316270 @default.
- W2891675981 creator A5072888106 @default.
- W2891675981 creator A5080723533 @default.
- W2891675981 creator A5085347016 @default.
- W2891675981 date "2018-09-07" @default.
- W2891675981 modified "2023-10-13" @default.
- W2891675981 title "Novel analgesic ω-conotoxins from the vermivorous cone snail Conus moncuri provide new insights into the evolution of conopeptides" @default.
- W2891675981 cites W1481413807 @default.
- W2891675981 cites W1589295940 @default.
- W2891675981 cites W1594133400 @default.
- W2891675981 cites W1682432998 @default.
- W2891675981 cites W1891909628 @default.
- W2891675981 cites W1935006995 @default.
- W2891675981 cites W1966349755 @default.
- W2891675981 cites W1966765310 @default.
- W2891675981 cites W1967382991 @default.
- W2891675981 cites W1969344024 @default.
- W2891675981 cites W1975891442 @default.
- W2891675981 cites W1976301354 @default.
- W2891675981 cites W1977634797 @default.
- W2891675981 cites W1995017064 @default.
- W2891675981 cites W1996186559 @default.
- W2891675981 cites W2002195659 @default.
- W2891675981 cites W2003703108 @default.
- W2891675981 cites W2004651435 @default.
- W2891675981 cites W2006441514 @default.
- W2891675981 cites W2022813014 @default.
- W2891675981 cites W2032529224 @default.
- W2891675981 cites W2038531512 @default.
- W2891675981 cites W2040588337 @default.
- W2891675981 cites W2048956541 @default.
- W2891675981 cites W2052933818 @default.
- W2891675981 cites W2056818917 @default.
- W2891675981 cites W2057257888 @default.
- W2891675981 cites W2057584533 @default.
- W2891675981 cites W2061761037 @default.
- W2891675981 cites W2062488318 @default.
- W2891675981 cites W2067924690 @default.
- W2891675981 cites W2077501370 @default.
- W2891675981 cites W2081544730 @default.
- W2891675981 cites W2083030813 @default.
- W2891675981 cites W2084126799 @default.
- W2891675981 cites W2089169743 @default.
- W2891675981 cites W2092424786 @default.
- W2891675981 cites W2096431999 @default.
- W2891675981 cites W2098746681 @default.
- W2891675981 cites W2100975785 @default.
- W2891675981 cites W2112418305 @default.
- W2891675981 cites W2116020741 @default.
- W2891675981 cites W2119057275 @default.
- W2891675981 cites W2122054000 @default.
- W2891675981 cites W2130064123 @default.
- W2891675981 cites W2133430337 @default.
- W2891675981 cites W2134571618 @default.
- W2891675981 cites W2137015675 @default.
- W2891675981 cites W2146837416 @default.
- W2891675981 cites W2147533449 @default.
- W2891675981 cites W2150713801 @default.
- W2891675981 cites W2151024126 @default.
- W2891675981 cites W2152791101 @default.
- W2891675981 cites W2154714625 @default.
- W2891675981 cites W2157568294 @default.
- W2891675981 cites W2160386479 @default.
- W2891675981 cites W2160796763 @default.
- W2891675981 cites W2170338669 @default.
- W2891675981 cites W2172205732 @default.
- W2891675981 cites W2397676117 @default.
- W2891675981 cites W2400232991 @default.
- W2891675981 cites W2512185988 @default.
- W2891675981 cites W2792530372 @default.
- W2891675981 cites W4242729757 @default.
- W2891675981 doi "https://doi.org/10.1038/s41598-018-31245-4" @default.
- W2891675981 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6128854" @default.
- W2891675981 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30194442" @default.
- W2891675981 hasPublicationYear "2018" @default.
- W2891675981 type Work @default.
- W2891675981 sameAs 2891675981 @default.
- W2891675981 citedByCount "18" @default.
- W2891675981 countsByYear W28916759812019 @default.
- W2891675981 countsByYear W28916759812020 @default.
- W2891675981 countsByYear W28916759812021 @default.
- W2891675981 countsByYear W28916759812022 @default.
- W2891675981 crossrefType "journal-article" @default.
- W2891675981 hasAuthorship W2891675981A5000965397 @default.
- W2891675981 hasAuthorship W2891675981A5005160521 @default.
- W2891675981 hasAuthorship W2891675981A5025386235 @default.
- W2891675981 hasAuthorship W2891675981A5025539645 @default.
- W2891675981 hasAuthorship W2891675981A5032618006 @default.
- W2891675981 hasAuthorship W2891675981A5032924771 @default.
- W2891675981 hasAuthorship W2891675981A5033658079 @default.