Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891689319> ?p ?o ?g. }
- W2891689319 abstract "Background: Many gene variants modulate the individual perception of pain and possibly also its persistence. The limited selection of single functional variants is increasingly being replaced by analyses of the full coding and regulatory sequences of pain-relevant genes accessible by means of next generation sequencing (NGS). Methods: An NGS panel was created for a set of 77 human genes selected following different lines of evidence supporting their role in persisting pain. To address the role of these candidate genes, we established a sequencing assay based on a custom AmpliSeqTM panel to assess the exomic sequences in 72 subjects of Caucasian ethnicity. To identify the systems biology of the genes, the biological functions associated with these genes were assessed by means of a computational over-representation analysis. Results: Sequencing generated a median of 2.85 ⋅ 106 reads per run with a mean depth close to 200 reads, mean read length of 205 called bases and an average chip loading of 71%. A total of 3,185 genetic variants were called. A computational functional genomics analysis indicated that the proposed NGS gene panel covers biological processes identified previously as characterizing the functional genomics of persisting pain. Conclusion: Results of the NGS assay suggested that the produced nucleotide sequences are comparable to those earned with the classical Sanger sequencing technique. The assay is applicable for small to large-scale experimental setups to target the accessing of information about any nucleotide within the addressed genes in a study cohort." @default.
- W2891689319 created "2018-09-27" @default.
- W2891689319 creator A5017400006 @default.
- W2891689319 creator A5033460624 @default.
- W2891689319 creator A5052199048 @default.
- W2891689319 creator A5076070835 @default.
- W2891689319 creator A5079793229 @default.
- W2891689319 date "2018-09-19" @default.
- W2891689319 modified "2023-10-14" @default.
- W2891689319 title "Development of an AmpliSeqTM Panel for Next-Generation Sequencing of a Set of Genetic Predictors of Persisting Pain" @default.
- W2891689319 cites W116417404 @default.
- W2891689319 cites W12310695 @default.
- W2891689319 cites W1483719585 @default.
- W2891689319 cites W1500349624 @default.
- W2891689319 cites W1504880924 @default.
- W2891689319 cites W1522123111 @default.
- W2891689319 cites W1567785039 @default.
- W2891689319 cites W1594871287 @default.
- W2891689319 cites W1598563927 @default.
- W2891689319 cites W160295494 @default.
- W2891689319 cites W1687596153 @default.
- W2891689319 cites W1817577608 @default.
- W2891689319 cites W1823900868 @default.
- W2891689319 cites W1848754477 @default.
- W2891689319 cites W1897762459 @default.
- W2891689319 cites W189910528 @default.
- W2891689319 cites W1906660686 @default.
- W2891689319 cites W192884275 @default.
- W2891689319 cites W1942045556 @default.
- W2891689319 cites W1964294716 @default.
- W2891689319 cites W1964601303 @default.
- W2891689319 cites W1965522578 @default.
- W2891689319 cites W1965917335 @default.
- W2891689319 cites W1966247889 @default.
- W2891689319 cites W1967568763 @default.
- W2891689319 cites W1967816257 @default.
- W2891689319 cites W1968242570 @default.
- W2891689319 cites W1968370892 @default.
- W2891689319 cites W1968714225 @default.
- W2891689319 cites W1969384722 @default.
- W2891689319 cites W1969430072 @default.
- W2891689319 cites W1970160843 @default.
- W2891689319 cites W1970441985 @default.
- W2891689319 cites W1970549297 @default.
- W2891689319 cites W1972353474 @default.
- W2891689319 cites W1975032608 @default.
- W2891689319 cites W1975172757 @default.
- W2891689319 cites W1975702320 @default.
- W2891689319 cites W1977321427 @default.
- W2891689319 cites W1978033978 @default.
- W2891689319 cites W1979609462 @default.
- W2891689319 cites W1979664847 @default.
- W2891689319 cites W1980857831 @default.
- W2891689319 cites W1981302370 @default.
- W2891689319 cites W1982215148 @default.
- W2891689319 cites W1984578218 @default.
- W2891689319 cites W1988209540 @default.
- W2891689319 cites W1988787218 @default.
- W2891689319 cites W1988973588 @default.
- W2891689319 cites W1991281518 @default.
- W2891689319 cites W1991344521 @default.
- W2891689319 cites W1991563355 @default.
- W2891689319 cites W1992557727 @default.
- W2891689319 cites W1994611071 @default.
- W2891689319 cites W1994722059 @default.
- W2891689319 cites W1994781970 @default.
- W2891689319 cites W1995019701 @default.
- W2891689319 cites W1995841899 @default.
- W2891689319 cites W1997921822 @default.
- W2891689319 cites W1999075283 @default.
- W2891689319 cites W2000084874 @default.
- W2891689319 cites W2000272921 @default.
- W2891689319 cites W2000344242 @default.
- W2891689319 cites W2002612293 @default.
- W2891689319 cites W2003404396 @default.
- W2891689319 cites W2004219505 @default.
- W2891689319 cites W2005649020 @default.
- W2891689319 cites W2006571737 @default.
- W2891689319 cites W2009717590 @default.
- W2891689319 cites W2010022125 @default.
- W2891689319 cites W2010161770 @default.
- W2891689319 cites W2010323946 @default.
- W2891689319 cites W2011834292 @default.
- W2891689319 cites W2012743573 @default.
- W2891689319 cites W2013079369 @default.
- W2891689319 cites W2013241897 @default.
- W2891689319 cites W2014973557 @default.
- W2891689319 cites W2016762800 @default.
- W2891689319 cites W2018823565 @default.
- W2891689319 cites W2019272591 @default.
- W2891689319 cites W2019765121 @default.
- W2891689319 cites W2022797906 @default.
- W2891689319 cites W2024214083 @default.
- W2891689319 cites W2026039752 @default.
- W2891689319 cites W2027873788 @default.
- W2891689319 cites W2030501710 @default.
- W2891689319 cites W2030711769 @default.
- W2891689319 cites W2030762826 @default.
- W2891689319 cites W2031979328 @default.
- W2891689319 cites W2032273455 @default.