FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891689731> ?p ?o ?g. }

• W2891689731 endingPage "1277" @default.
• W2891689731 startingPage "1267" @default.
• W2891689731 abstract "Background aims Regenerative medicine strategies based on cell therapy are considered a promising approach to repair bone defects. The aims of this study were to evaluate the effect of subculturing on the osteogenic potential of osteoblasts derived from newborn rat calvaria and the effect of these osteoblasts on bone repair of rat calvaria defects. Methods Cells were obtained from 50 newborn rat calvaria, and primary osteoblasts (OB) were compared with first passage (OB-P1) in terms of osteogenic potential by assaying cell proliferation, alkaline phosphatase (ALP) activity, extracellular matrix mineralization and gene expression of the osteoblastic markers RUNX2, ALP, osteocalcin and bone sialoprotein. Then, 5-mm calvaria defects were created in 24 Wistar rats, and after 2 weeks, they were locally injected with 50 µL of phosphate-buffered saline containing either 5 × 106 osteoblasts (OB-P1, n = 12) or no cells (control, n = 12). Four weeks post-injection, the bone formation was evaluated by micro-computed tomography and histological analyses. Data were compared by analysis of variance, followed by the Student-Newman-Keuls's test or Student's t-test (P ≤ 0.05). Results OB-P1 showed high proliferation and ALP activity, and despite the reduced gene expression of osteoblastic markers and extracellular matrix mineralization compared with OB, they displayed osteogenic potential, being a good choice for injection into calvaria defects. The micro-tomographic and histological data showed that defects treated with OB-P1 presented higher bone formation compared with control defects. Discussion Our results indicate that cells derived from newborn rat calvaria retain osteoblastic characteristics after subculturing and that these osteoblasts stimulate bone repair in a rat calvaria defect model." @default.
• W2891689731 created "2018-09-27" @default.
• W2891689731 creator A5014481104 @default.
• W2891689731 creator A5037998141 @default.
• W2891689731 creator A5054432013 @default.
• W2891689731 creator A5057897128 @default.
• W2891689731 creator A5067092603 @default.
• W2891689731 creator A5086522266 @default.
• W2891689731 creator A5089458838 @default.
• W2891689731 date "2018-10-01" @default.
• W2891689731 modified "2023-10-01" @default.
• W2891689731 title "Effect of cell therapy with allogeneic osteoblasts on bone repair of rat calvaria defects" @default.
• W2891689731 cites W1876261563 @default.
• W2891689731 cites W1964481878 @default.
• W2891689731 cites W1979478755 @default.
• W2891689731 cites W1986872335 @default.
• W2891689731 cites W1988221250 @default.
• W2891689731 cites W2020616952 @default.
• W2891689731 cites W2031193236 @default.
• W2891689731 cites W2037272700 @default.
• W2891689731 cites W2042357573 @default.
• W2891689731 cites W2048993028 @default.
• W2891689731 cites W2055690022 @default.
• W2891689731 cites W2057926683 @default.
• W2891689731 cites W2065323519 @default.
• W2891689731 cites W2068596665 @default.
• W2891689731 cites W2070754567 @default.
• W2891689731 cites W2073130464 @default.
• W2891689731 cites W2086118526 @default.
• W2891689731 cites W2093191361 @default.
• W2891689731 cites W2106060595 @default.
• W2891689731 cites W2114918609 @default.
• W2891689731 cites W2118689481 @default.
• W2891689731 cites W2187781852 @default.
• W2891689731 cites W2264861089 @default.
• W2891689731 cites W2559081809 @default.
• W2891689731 cites W2568419125 @default.
• W2891689731 cites W2609534315 @default.
• W2891689731 cites W2621127213 @default.
• W2891689731 doi "https://doi.org/10.1016/j.jcyt.2018.06.010" @default.
• W2891689731 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30196010" @default.
• W2891689731 hasPublicationYear "2018" @default.
• W2891689731 type Work @default.
• W2891689731 sameAs 2891689731 @default.
• W2891689731 citedByCount "10" @default.
• W2891689731 countsByYear W28916897312019 @default.
• W2891689731 countsByYear W28916897312020 @default.
• W2891689731 countsByYear W28916897312021 @default.
• W2891689731 countsByYear W28916897312022 @default.
• W2891689731 countsByYear W28916897312023 @default.
• W2891689731 crossrefType "journal-article" @default.
• W2891689731 hasAuthorship W2891689731A5014481104 @default.
• W2891689731 hasAuthorship W2891689731A5037998141 @default.
• W2891689731 hasAuthorship W2891689731A5054432013 @default.
• W2891689731 hasAuthorship W2891689731A5057897128 @default.
• W2891689731 hasAuthorship W2891689731A5067092603 @default.
• W2891689731 hasAuthorship W2891689731A5086522266 @default.
• W2891689731 hasAuthorship W2891689731A5089458838 @default.
• W2891689731 hasConcept C105702510 @default.
• W2891689731 hasConcept C126322002 @default.
• W2891689731 hasConcept C131075544 @default.
• W2891689731 hasConcept C134018914 @default.
• W2891689731 hasConcept C160160445 @default.
• W2891689731 hasConcept C16685009 @default.
• W2891689731 hasConcept C181199279 @default.
• W2891689731 hasConcept C185592680 @default.
• W2891689731 hasConcept C189165786 @default.
• W2891689731 hasConcept C202751555 @default.
• W2891689731 hasConcept C2776432469 @default.
• W2891689731 hasConcept C2778260815 @default.
• W2891689731 hasConcept C2779918246 @default.
• W2891689731 hasConcept C55493867 @default.
• W2891689731 hasConcept C71924100 @default.
• W2891689731 hasConcept C8337478 @default.
• W2891689731 hasConceptScore W2891689731C105702510 @default.
• W2891689731 hasConceptScore W2891689731C126322002 @default.
• W2891689731 hasConceptScore W2891689731C131075544 @default.
• W2891689731 hasConceptScore W2891689731C134018914 @default.
• W2891689731 hasConceptScore W2891689731C160160445 @default.
• W2891689731 hasConceptScore W2891689731C16685009 @default.
• W2891689731 hasConceptScore W2891689731C181199279 @default.
• W2891689731 hasConceptScore W2891689731C185592680 @default.
• W2891689731 hasConceptScore W2891689731C189165786 @default.
• W2891689731 hasConceptScore W2891689731C202751555 @default.
• W2891689731 hasConceptScore W2891689731C2776432469 @default.
• W2891689731 hasConceptScore W2891689731C2778260815 @default.
• W2891689731 hasConceptScore W2891689731C2779918246 @default.
• W2891689731 hasConceptScore W2891689731C55493867 @default.
• W2891689731 hasConceptScore W2891689731C71924100 @default.
• W2891689731 hasConceptScore W2891689731C8337478 @default.
• W2891689731 hasIssue "10" @default.
• W2891689731 hasLocation W28916897311 @default.
• W2891689731 hasLocation W28916897312 @default.
• W2891689731 hasOpenAccess W2891689731 @default.
• W2891689731 hasPrimaryLocation W28916897311 @default.
• W2891689731 hasRelatedWork W2028553359 @default.
• W2891689731 hasRelatedWork W2044971600 @default.
• W2891689731 hasRelatedWork W2049109419 @default.

• next