Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891697602> ?p ?o ?g. }
- W2891697602 endingPage "155" @default.
- W2891697602 startingPage "149" @default.
- W2891697602 abstract "Chlorella variabilis, a symbiotic alga, is usually present in the cytoplasm of Paramecium bursaria, although it can be cultured in host-free conditions. Morphological and chemical properties of its cell wall were compared between its free-living and symbiotic states. Transmission electron microscopy (quick-freezing and freeze-substitution methods) revealed that the cell wall thickness of symbiotic C. variabilis was reduced to about half that of the free-living one. Chemical properties of the cell wall were examined by treatment with three fluorescent reagents (calcofluor white M2R, FITC-WGA, and FITC-LFA) having specific binding affinities to different polysaccharides. When the algae were re-introduced into Paramecium host cells, calcofluor fluorescence intensity reduced by about 50%. Calcofluor stains β-d-glucopyranose polysaccharides such as cellulose, N-acetylglucosamine, sialic acid, and glycosaminoglycans. Because treatment with cellulase showed no effect on calcofluor fluorescence intensity, we consider that cellulose is not majorly responsible for the stainability of calcofluor. Staining intensities of FITC-WGA and FITC-LFA were similar in the free-living and symbiotic conditions, suggesting that N-acetylglucosamine and sialic acid are also not responsible for the reduction in the stainability of calcofluor associated with intracellular symbiosis. The amount of glycosaminoglycans on the cell wall may decrease in C. variabilis present in the cytoplasm of P. bursaria." @default.
- W2891697602 created "2018-09-27" @default.
- W2891697602 creator A5031022142 @default.
- W2891697602 creator A5034193278 @default.
- W2891697602 creator A5044891281 @default.
- W2891697602 creator A5081445851 @default.
- W2891697602 date "2018-10-01" @default.
- W2891697602 modified "2023-09-23" @default.
- W2891697602 title "Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea)" @default.
- W2891697602 cites W1691251583 @default.
- W2891697602 cites W1880872174 @default.
- W2891697602 cites W1925234808 @default.
- W2891697602 cites W1965894664 @default.
- W2891697602 cites W1974288891 @default.
- W2891697602 cites W1974572488 @default.
- W2891697602 cites W1994527211 @default.
- W2891697602 cites W1998407827 @default.
- W2891697602 cites W2015830910 @default.
- W2891697602 cites W2019199044 @default.
- W2891697602 cites W2029310489 @default.
- W2891697602 cites W2035718072 @default.
- W2891697602 cites W2041009353 @default.
- W2891697602 cites W2049528392 @default.
- W2891697602 cites W2059705979 @default.
- W2891697602 cites W2066653865 @default.
- W2891697602 cites W2070019284 @default.
- W2891697602 cites W2070354830 @default.
- W2891697602 cites W2105814837 @default.
- W2891697602 cites W2116342058 @default.
- W2891697602 cites W2125983377 @default.
- W2891697602 cites W2127911529 @default.
- W2891697602 cites W2137015675 @default.
- W2891697602 cites W2145319301 @default.
- W2891697602 cites W2154947150 @default.
- W2891697602 cites W2165158280 @default.
- W2891697602 cites W2319533931 @default.
- W2891697602 cites W2606695790 @default.
- W2891697602 cites W270842019 @default.
- W2891697602 doi "https://doi.org/10.1016/j.ejop.2018.09.003" @default.
- W2891697602 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30286318" @default.
- W2891697602 hasPublicationYear "2018" @default.
- W2891697602 type Work @default.
- W2891697602 sameAs 2891697602 @default.
- W2891697602 citedByCount "5" @default.
- W2891697602 countsByYear W28916976022019 @default.
- W2891697602 countsByYear W28916976022021 @default.
- W2891697602 countsByYear W28916976022022 @default.
- W2891697602 countsByYear W28916976022023 @default.
- W2891697602 crossrefType "journal-article" @default.
- W2891697602 hasAuthorship W2891697602A5031022142 @default.
- W2891697602 hasAuthorship W2891697602A5034193278 @default.
- W2891697602 hasAuthorship W2891697602A5044891281 @default.
- W2891697602 hasAuthorship W2891697602A5081445851 @default.
- W2891697602 hasBestOaLocation W28916976021 @default.
- W2891697602 hasConcept C100817775 @default.
- W2891697602 hasConcept C125235067 @default.
- W2891697602 hasConcept C12554922 @default.
- W2891697602 hasConcept C190062978 @default.
- W2891697602 hasConcept C2777563530 @default.
- W2891697602 hasConcept C2779114684 @default.
- W2891697602 hasConcept C2779669040 @default.
- W2891697602 hasConcept C2780536333 @default.
- W2891697602 hasConcept C523546767 @default.
- W2891697602 hasConcept C54355233 @default.
- W2891697602 hasConcept C55493867 @default.
- W2891697602 hasConcept C559758991 @default.
- W2891697602 hasConcept C59822182 @default.
- W2891697602 hasConcept C86803240 @default.
- W2891697602 hasConceptScore W2891697602C100817775 @default.
- W2891697602 hasConceptScore W2891697602C125235067 @default.
- W2891697602 hasConceptScore W2891697602C12554922 @default.
- W2891697602 hasConceptScore W2891697602C190062978 @default.
- W2891697602 hasConceptScore W2891697602C2777563530 @default.
- W2891697602 hasConceptScore W2891697602C2779114684 @default.
- W2891697602 hasConceptScore W2891697602C2779669040 @default.
- W2891697602 hasConceptScore W2891697602C2780536333 @default.
- W2891697602 hasConceptScore W2891697602C523546767 @default.
- W2891697602 hasConceptScore W2891697602C54355233 @default.
- W2891697602 hasConceptScore W2891697602C55493867 @default.
- W2891697602 hasConceptScore W2891697602C559758991 @default.
- W2891697602 hasConceptScore W2891697602C59822182 @default.
- W2891697602 hasConceptScore W2891697602C86803240 @default.
- W2891697602 hasFunder F4320334764 @default.
- W2891697602 hasLocation W28916976021 @default.
- W2891697602 hasLocation W28916976022 @default.
- W2891697602 hasOpenAccess W2891697602 @default.
- W2891697602 hasPrimaryLocation W28916976021 @default.
- W2891697602 hasRelatedWork W1493252974 @default.
- W2891697602 hasRelatedWork W1996656972 @default.
- W2891697602 hasRelatedWork W2031145294 @default.
- W2891697602 hasRelatedWork W2039238015 @default.
- W2891697602 hasRelatedWork W2040240987 @default.
- W2891697602 hasRelatedWork W2086105059 @default.
- W2891697602 hasRelatedWork W2092917152 @default.
- W2891697602 hasRelatedWork W2110255731 @default.
- W2891697602 hasRelatedWork W2891697602 @default.
- W2891697602 hasRelatedWork W4255277642 @default.
- W2891697602 hasVolume "66" @default.