Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891702191> ?p ?o ?g. }
- W2891702191 endingPage "803" @default.
- W2891702191 startingPage "795" @default.
- W2891702191 abstract "Abstract BACKGROUND The immobilization of multiple oxidoreductases has attracted a lot of research interest for its industrial application in eco‐friendly production. Here, a novel immobilization strategy was developed using manganese ion chelated linear polyethyleneimine as artificial hybrid scaffold that allowed self‐assembly of bifunctional oxidoreductase (glycerol dehydrogenase‐NADH oxidase) via multipoint coordination interaction. RESULT The immobilization formed a highly ordered polymer–metal–enzyme complex structure shaped like a leaf vein with 200‐nm‐wide nanowires. Preliminary results showed that the immobilization could not only retain but substantially increased the biological activity of the immobilized enzyme by 8.2‐fold. The equilibrium concentration of the reaction product DHA also was increased by 4.5‐fold compared with that of the free enzyme, exhibiting higher elimination efficiency of product inhibition compared with other enzyme immobilization strategy using nanoparticles or activated agarose beads. At the same time, linear polyethyleneimine (LPEI)–manganese scaffolds could prevent enzyme subunit disassociation and stabilize the active multimeric form, which improved catalytic condition adaption and thermal stability of target enzyme. CONCLUSION These results indicated a great potential of polymer–metal–enzyme hybrid in the enzyme immobilization and have provided a simple and efficient strategy for the immobilization of multiple oxidoreductases. © 2018 Society of Chemical Industry" @default.
- W2891702191 created "2018-09-27" @default.
- W2891702191 creator A5005614590 @default.
- W2891702191 creator A5014954540 @default.
- W2891702191 creator A5034896707 @default.
- W2891702191 creator A5041677976 @default.
- W2891702191 creator A5046173951 @default.
- W2891702191 creator A5065402568 @default.
- W2891702191 creator A5071437752 @default.
- W2891702191 creator A5081884771 @default.
- W2891702191 date "2018-11-02" @default.
- W2891702191 modified "2023-10-17" @default.
- W2891702191 title "Preparation and evaluation of a polymer-metal-enzyme hybrid nanowire for the immobilization of multiple oxidoreductases" @default.
- W2891702191 cites W126959334 @default.
- W2891702191 cites W1629931862 @default.
- W2891702191 cites W1839959619 @default.
- W2891702191 cites W1965560011 @default.
- W2891702191 cites W1969985867 @default.
- W2891702191 cites W1979519758 @default.
- W2891702191 cites W1984180361 @default.
- W2891702191 cites W1986646577 @default.
- W2891702191 cites W2002024464 @default.
- W2891702191 cites W2005555698 @default.
- W2891702191 cites W2030022295 @default.
- W2891702191 cites W2036479081 @default.
- W2891702191 cites W2040889988 @default.
- W2891702191 cites W2042931139 @default.
- W2891702191 cites W2051419400 @default.
- W2891702191 cites W2078288803 @default.
- W2891702191 cites W2083058120 @default.
- W2891702191 cites W2089157631 @default.
- W2891702191 cites W2095963233 @default.
- W2891702191 cites W2105590412 @default.
- W2891702191 cites W2114032433 @default.
- W2891702191 cites W2118131251 @default.
- W2891702191 cites W2149603427 @default.
- W2891702191 cites W2149704924 @default.
- W2891702191 cites W2158543301 @default.
- W2891702191 cites W2303875806 @default.
- W2891702191 cites W2305514978 @default.
- W2891702191 cites W2316966637 @default.
- W2891702191 cites W2326186751 @default.
- W2891702191 cites W2328363044 @default.
- W2891702191 cites W2330733968 @default.
- W2891702191 cites W2335475385 @default.
- W2891702191 cites W2408768369 @default.
- W2891702191 cites W2466291603 @default.
- W2891702191 cites W2539825157 @default.
- W2891702191 cites W2562647913 @default.
- W2891702191 cites W2563573909 @default.
- W2891702191 cites W2576153484 @default.
- W2891702191 cites W2580604923 @default.
- W2891702191 cites W2583901564 @default.
- W2891702191 cites W2607513260 @default.
- W2891702191 cites W2674118028 @default.
- W2891702191 cites W2692764156 @default.
- W2891702191 cites W2757715201 @default.
- W2891702191 cites W4236657280 @default.
- W2891702191 cites W4248710825 @default.
- W2891702191 cites W4361865023 @default.
- W2891702191 doi "https://doi.org/10.1002/jctb.5825" @default.
- W2891702191 hasPublicationYear "2018" @default.
- W2891702191 type Work @default.
- W2891702191 sameAs 2891702191 @default.
- W2891702191 citedByCount "5" @default.
- W2891702191 countsByYear W28917021912019 @default.
- W2891702191 countsByYear W28917021912020 @default.
- W2891702191 countsByYear W28917021912021 @default.
- W2891702191 countsByYear W28917021912022 @default.
- W2891702191 crossrefType "journal-article" @default.
- W2891702191 hasAuthorship W2891702191A5005614590 @default.
- W2891702191 hasAuthorship W2891702191A5014954540 @default.
- W2891702191 hasAuthorship W2891702191A5034896707 @default.
- W2891702191 hasAuthorship W2891702191A5041677976 @default.
- W2891702191 hasAuthorship W2891702191A5046173951 @default.
- W2891702191 hasAuthorship W2891702191A5065402568 @default.
- W2891702191 hasAuthorship W2891702191A5071437752 @default.
- W2891702191 hasAuthorship W2891702191A5081884771 @default.
- W2891702191 hasConcept C161790260 @default.
- W2891702191 hasConcept C178790620 @default.
- W2891702191 hasConcept C181199279 @default.
- W2891702191 hasConcept C185592680 @default.
- W2891702191 hasConcept C199164860 @default.
- W2891702191 hasConcept C21951064 @default.
- W2891702191 hasConcept C2778476535 @default.
- W2891702191 hasConcept C2779268744 @default.
- W2891702191 hasConcept C2780443040 @default.
- W2891702191 hasConcept C33633552 @default.
- W2891702191 hasConcept C521977710 @default.
- W2891702191 hasConcept C528890316 @default.
- W2891702191 hasConcept C544153396 @default.
- W2891702191 hasConcept C55493867 @default.
- W2891702191 hasConceptScore W2891702191C161790260 @default.
- W2891702191 hasConceptScore W2891702191C178790620 @default.
- W2891702191 hasConceptScore W2891702191C181199279 @default.
- W2891702191 hasConceptScore W2891702191C185592680 @default.
- W2891702191 hasConceptScore W2891702191C199164860 @default.
- W2891702191 hasConceptScore W2891702191C21951064 @default.