Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891708521> ?p ?o ?g. }
- W2891708521 endingPage "161" @default.
- W2891708521 startingPage "154" @default.
- W2891708521 abstract "As alkaline phosphatase (ALP) plays crucial roles in disease warning and dephosphorylation-related cellular regulation, it is widely recognized as an important biomarker for clinical diagnosis. In this work, we proposed a facile colorimetric assay based on phosphate anion-quenched oxidase-mimicking activity of Ce(Ⅳ) ions for sensitive and selective detection of ALP activity. Free Ce(Ⅳ) ions exhibited a strong oxidase-like capability (providing a 40-fold catalytic turnover number compared with CeO2) to catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into its blue product TMBox mediated by dissolved O2 at neutral pH, thus triggering a remarkable color reaction visually. When PO43− was added, its strong affinity to Ce(Ⅳ) ions rapidly precipitated the free Ce(Ⅳ) ions, resulting in the quenching of their enzymatic ability. Given that ALP catalyzed the hydrolysis of adenosine triphosphate to produce PO43−, determination of ALP activity could be achieved using the colorimetric assay with no need of complicated instrumentation and protocol. As demonstrated, our assay offered a highly sensitive readout for ALP activity in two linear scopes of 0–50 U L−1 and 50–250 U L−1, providing a detection limit down to 2.3 U L−1. Besides, it could provide specific response toward ALP against other enzymes and biological species. Furthermore, the developed assay was successfully applied to evaluate ALP activity in human plasma accurately and reliably, indicating its great promise as a powerful and convenient tool for monitoring of ALP activity in clinical practice." @default.
- W2891708521 created "2018-09-27" @default.
- W2891708521 creator A5032497440 @default.
- W2891708521 creator A5037095934 @default.
- W2891708521 creator A5039901280 @default.
- W2891708521 creator A5043998061 @default.
- W2891708521 creator A5048030742 @default.
- W2891708521 creator A5074021241 @default.
- W2891708521 date "2018-12-01" @default.
- W2891708521 modified "2023-10-17" @default.
- W2891708521 title "Sensitive and selective colorimetric detection of alkaline phosphatase activity based on phosphate anion-quenched oxidase-mimicking activity of Ce(Ⅳ) ions" @default.
- W2891708521 cites W1870790255 @default.
- W2891708521 cites W1934005039 @default.
- W2891708521 cites W1976652877 @default.
- W2891708521 cites W1984662357 @default.
- W2891708521 cites W1991161810 @default.
- W2891708521 cites W2011676749 @default.
- W2891708521 cites W2012268544 @default.
- W2891708521 cites W2023477615 @default.
- W2891708521 cites W2030829280 @default.
- W2891708521 cites W2039880215 @default.
- W2891708521 cites W2047421240 @default.
- W2891708521 cites W2048105979 @default.
- W2891708521 cites W2049463639 @default.
- W2891708521 cites W2053282636 @default.
- W2891708521 cites W2054179978 @default.
- W2891708521 cites W2066984582 @default.
- W2891708521 cites W2068615956 @default.
- W2891708521 cites W2069395700 @default.
- W2891708521 cites W2080356514 @default.
- W2891708521 cites W2086673935 @default.
- W2891708521 cites W2091688144 @default.
- W2891708521 cites W2105388112 @default.
- W2891708521 cites W2120028559 @default.
- W2891708521 cites W2170913114 @default.
- W2891708521 cites W2187994689 @default.
- W2891708521 cites W2189180879 @default.
- W2891708521 cites W2189968318 @default.
- W2891708521 cites W2226131629 @default.
- W2891708521 cites W2259373292 @default.
- W2891708521 cites W2313826636 @default.
- W2891708521 cites W2315077945 @default.
- W2891708521 cites W2321317665 @default.
- W2891708521 cites W2325779013 @default.
- W2891708521 cites W2330791167 @default.
- W2891708521 cites W2332509679 @default.
- W2891708521 cites W2332536232 @default.
- W2891708521 cites W2337627606 @default.
- W2891708521 cites W2338371037 @default.
- W2891708521 cites W2461787424 @default.
- W2891708521 cites W2465938557 @default.
- W2891708521 cites W2520553409 @default.
- W2891708521 cites W2532290176 @default.
- W2891708521 cites W2534326905 @default.
- W2891708521 cites W2544786672 @default.
- W2891708521 cites W2550297593 @default.
- W2891708521 cites W2555317635 @default.
- W2891708521 cites W2586484796 @default.
- W2891708521 cites W2591395609 @default.
- W2891708521 cites W2592498980 @default.
- W2891708521 cites W2594203659 @default.
- W2891708521 cites W2594267852 @default.
- W2891708521 cites W2604679019 @default.
- W2891708521 cites W2761621912 @default.
- W2891708521 cites W2791743752 @default.
- W2891708521 cites W2792118519 @default.
- W2891708521 cites W4230713761 @default.
- W2891708521 cites W639302564 @default.
- W2891708521 doi "https://doi.org/10.1016/j.aca.2018.09.045" @default.
- W2891708521 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30442397" @default.
- W2891708521 hasPublicationYear "2018" @default.
- W2891708521 type Work @default.
- W2891708521 sameAs 2891708521 @default.
- W2891708521 citedByCount "50" @default.
- W2891708521 countsByYear W28917085212019 @default.
- W2891708521 countsByYear W28917085212020 @default.
- W2891708521 countsByYear W28917085212021 @default.
- W2891708521 countsByYear W28917085212022 @default.
- W2891708521 countsByYear W28917085212023 @default.
- W2891708521 crossrefType "journal-article" @default.
- W2891708521 hasAuthorship W2891708521A5032497440 @default.
- W2891708521 hasAuthorship W2891708521A5037095934 @default.
- W2891708521 hasAuthorship W2891708521A5039901280 @default.
- W2891708521 hasAuthorship W2891708521A5043998061 @default.
- W2891708521 hasAuthorship W2891708521A5048030742 @default.
- W2891708521 hasAuthorship W2891708521A5074021241 @default.
- W2891708521 hasConcept C119128265 @default.
- W2891708521 hasConcept C121332964 @default.
- W2891708521 hasConcept C121745418 @default.
- W2891708521 hasConcept C145148216 @default.
- W2891708521 hasConcept C160160445 @default.
- W2891708521 hasConcept C178666793 @default.
- W2891708521 hasConcept C178790620 @default.
- W2891708521 hasConcept C181199279 @default.
- W2891708521 hasConcept C181912034 @default.
- W2891708521 hasConcept C185592680 @default.
- W2891708521 hasConcept C2777132085 @default.
- W2891708521 hasConcept C38485361 @default.