FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891772303> ?p ?o ?g. }

• W2891772303 abstract "The CRISPR/Cas9 technology has opened the possibility for targeted genome editing in various organisms including diatom model organisms. One standard method for delivery of vectors to diatom cells is by biolistic particle bombardment. Recently delivery by conjugation was added to the tool-box. An important difference between these methods is that biolistic transformation results in transgene integration of vector DNA into the algae genome, whereas conjugative transformation allows the vector to be maintained as an episome in the recipient cells. In this study, we have used both transformation methods to deliver the CRISPR/Cas9 system to the marine diatom Phaeodactylum tricornutum aiming to induce mutations in a common target gene. This allowed us to compare the two CRISPR/Cas9 delivery systems with regard to mutation efficiency, and to assess potential problems connected to constitutive expression of Cas9. We found that the percentage of CRISPR-induced targeted biallelic mutations are similar for both methods, but an extended growth period might be needed to induce biallelic mutations when the CRISPR/Cas9 system is episomal. Independent of the CRISPR/Cas9 vector system, constitutive expression of Cas9 can cause re-editing of mutant lines with small indels. Complications associated with the biolistic transformation system like the permanent and random integration of foreign DNA into the host genome and unstable mutant lines caused by constitutive expression of Cas9 can be avoided using the episomal CRISPR/Cas9 system. The episomal vector can be eliminated from the diatom cells by removal of selection pressure, resulting in transient Cas9 expression and non-transgenic mutant lines. Depending on legislation, such lines might be considered as non-GMOs." @default.
• W2891772303 created "2018-09-27" @default.
• W2891772303 creator A5014828089 @default.
• W2891772303 creator A5022250069 @default.
• W2891772303 creator A5046372204 @default.
• W2891772303 creator A5049922665 @default.
• W2891772303 creator A5055791898 @default.
• W2891772303 date "2018-09-26" @default.
• W2891772303 modified "2023-10-17" @default.
• W2891772303 title "Transgene-free genome editing in marine algae by bacterial conjugation – comparison with biolistic CRISPR/Cas9 transformation" @default.
• W2891772303 cites W1975135854 @default.
• W2891772303 cites W1980201684 @default.
• W2891772303 cites W2000046950 @default.
• W2891772303 cites W2031468053 @default.
• W2891772303 cites W2036176224 @default.
• W2891772303 cites W2036577945 @default.
• W2891772303 cites W2039705224 @default.
• W2891772303 cites W2040125628 @default.
• W2891772303 cites W2053988575 @default.
• W2891772303 cites W2057847071 @default.
• W2891772303 cites W2064941324 @default.
• W2891772303 cites W2068651431 @default.
• W2891772303 cites W2073412341 @default.
• W2891772303 cites W2077659966 @default.
• W2891772303 cites W2119734711 @default.
• W2891772303 cites W2127313385 @default.
• W2891772303 cites W2129939060 @default.
• W2891772303 cites W2129993269 @default.
• W2891772303 cites W2131565851 @default.
• W2891772303 cites W2136309995 @default.
• W2891772303 cites W2145782793 @default.
• W2891772303 cites W2146349577 @default.
• W2891772303 cites W2148857684 @default.
• W2891772303 cites W2149548143 @default.
• W2891772303 cites W2153344788 @default.
• W2891772303 cites W2160586543 @default.
• W2891772303 cites W2168740468 @default.
• W2891772303 cites W2171359888 @default.
• W2891772303 cites W2224398381 @default.
• W2891772303 cites W2337748739 @default.
• W2891772303 cites W2343659782 @default.
• W2891772303 cites W2513603580 @default.
• W2891772303 cites W2604311524 @default.
• W2891772303 cites W2742850404 @default.
• W2891772303 cites W2782004254 @default.
• W2891772303 cites W2787911644 @default.
• W2891772303 cites W2951302782 @default.
• W2891772303 doi "https://doi.org/10.1038/s41598-018-32342-0" @default.
• W2891772303 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6158232" @default.
• W2891772303 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30258061" @default.
• W2891772303 hasPublicationYear "2018" @default.
• W2891772303 type Work @default.
• W2891772303 sameAs 2891772303 @default.
• W2891772303 citedByCount "48" @default.
• W2891772303 countsByYear W28917723032019 @default.
• W2891772303 countsByYear W28917723032020 @default.
• W2891772303 countsByYear W28917723032021 @default.
• W2891772303 countsByYear W28917723032022 @default.
• W2891772303 countsByYear W28917723032023 @default.
• W2891772303 crossrefType "journal-article" @default.
• W2891772303 hasAuthorship W2891772303A5014828089 @default.
• W2891772303 hasAuthorship W2891772303A5022250069 @default.
• W2891772303 hasAuthorship W2891772303A5046372204 @default.
• W2891772303 hasAuthorship W2891772303A5049922665 @default.
• W2891772303 hasAuthorship W2891772303A5055791898 @default.
• W2891772303 hasBestOaLocation W28917723031 @default.
• W2891772303 hasConcept C102230213 @default.
• W2891772303 hasConcept C104317684 @default.
• W2891772303 hasConcept C132455925 @default.
• W2891772303 hasConcept C141231307 @default.
• W2891772303 hasConcept C144501496 @default.
• W2891772303 hasConcept C204241405 @default.
• W2891772303 hasConcept C54355233 @default.
• W2891772303 hasConcept C70721500 @default.
• W2891772303 hasConcept C86803240 @default.
• W2891772303 hasConcept C98108389 @default.
• W2891772303 hasConceptScore W2891772303C102230213 @default.
• W2891772303 hasConceptScore W2891772303C104317684 @default.
• W2891772303 hasConceptScore W2891772303C132455925 @default.
• W2891772303 hasConceptScore W2891772303C141231307 @default.
• W2891772303 hasConceptScore W2891772303C144501496 @default.
• W2891772303 hasConceptScore W2891772303C204241405 @default.
• W2891772303 hasConceptScore W2891772303C54355233 @default.
• W2891772303 hasConceptScore W2891772303C70721500 @default.
• W2891772303 hasConceptScore W2891772303C86803240 @default.
• W2891772303 hasConceptScore W2891772303C98108389 @default.
• W2891772303 hasIssue "1" @default.
• W2891772303 hasLocation W28917723031 @default.
• W2891772303 hasLocation W28917723032 @default.
• W2891772303 hasLocation W28917723033 @default.
• W2891772303 hasLocation W28917723034 @default.
• W2891772303 hasOpenAccess W2891772303 @default.
• W2891772303 hasPrimaryLocation W28917723031 @default.
• W2891772303 hasRelatedWork W2138734944 @default.
• W2891772303 hasRelatedWork W2527347512 @default.
• W2891772303 hasRelatedWork W2770152187 @default.
• W2891772303 hasRelatedWork W2900771990 @default.
• W2891772303 hasRelatedWork W2943124855 @default.
• W2891772303 hasRelatedWork W2953290252 @default.
• W2891772303 hasRelatedWork W2982388533 @default.

• next