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• W2891784015 endingPage "468" @default.
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• W2891784015 abstract "Epithelial-mesenchymal transition (EMT) is a biological process during which cells lose their characteristic structure and biochemical properties then adopt typical features of a mesenchymal phenotype. Alterations in the morphology, structure, and mechanical properties of cells during EMT are associated with a series of pathological processes. In this work, atomic force microscopy (AFM) is used for investigating effects of TGF-β1 on morphology and mechanical properties of living bladder cancer cells (T24) during EMT for the first time. High-resolution topography and Young's modulus images of T24 living cell are obtained simultaneously. The results show that TGF-β1 is able to induce EMT, leading to the increased F-actin stress fibers and much higher Young's modulus values of T24 living cells. It reveals that the cytoskeletal-associated cell architecture is closely related to the mechanical dynamics of T24 cells during EMT. This work provides new insights into the changes of cell morphology and mechanical properties during EMT. It enables us to gain a deeper understanding of the growth, development and metastasis of the bladder cancer cell therefore it is of great significance for studying the pathological mechanism of cells at single-cell level." @default.
• W2891784015 created "2018-09-27" @default.
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• W2891784015 date "2019-01-01" @default.
• W2891784015 modified "2023-10-16" @default.
• W2891784015 title "Directly observing alterations of morphology and mechanical properties of living cancer cells with atomic force microscopy" @default.
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• W2891784015 doi "https://doi.org/10.1016/j.talanta.2018.09.008" @default.
• W2891784015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30262086" @default.
• W2891784015 hasPublicationYear "2019" @default.
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