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• W2891788346 endingPage "180955" @default.
• W2891788346 startingPage "180955" @default.
• W2891788346 abstract "Acute urinary tract infections (UTIs) are one of the most common nosocomial bacterial infections, which affect almost 50% of the population at least once in their lifetime. UTIs may lead to lethal consequences if they are left undiagnosed and not properly treated. Early, rapid and accurate uropathogens detection methods play a pivotal role in clinical process. In this work, a portable bacteria-grasping surface-enhanced Raman scattering (SERS) chip for identification of three species of uropathogens (Escherichia coli CFT 073, Pseudomonas aeruginosa PAO1 and Proteus mirabilis PRM1) directly from culture matrix was reported. The chip was firstly modified with a positively charged NH3+ group, which enables itself grasp the negatively charged bacterial cells through the electrostatic adsorption principle. After the bacterial cells were captured by the chip, concentrated Ag nanoparticles (NPs) were used to obtain their Raman fingerprint spectra with recognizable characteristic peaks and good reproducibility. With the help of chemometric method such as discriminant analysis (DA), the SERS-based chip allows a rapid, successful identification of three species of UTI bacteria with a minimal bacterial concentration (105 cells ml-1) required for clinical diagnostics. In addition, this chip could spot the bacterial SERS fingerprints information directly from LB culture medium and artificial urine without sample pre-treatment. The portable bacteria-grasping SERS-based chip provides a possibility for fast and easy detection of uropathogens, and viability of future development in healthcare applications." @default.
• W2891788346 created "2018-09-27" @default.
• W2891788346 creator A5003022208 @default.
• W2891788346 creator A5028809157 @default.
• W2891788346 creator A5074657153 @default.
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• W2891788346 date "2018-09-01" @default.
• W2891788346 modified "2023-10-03" @default.
• W2891788346 title "Portable bacteria-capturing chip for direct surface-enhanced Raman scattering identification of urinary tract infection pathogens" @default.
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• W2891788346 doi "https://doi.org/10.1098/rsos.180955" @default.
• W2891788346 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6170559" @default.
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• W2891788346 hasPublicationYear "2018" @default.
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