Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891802653> ?p ?o ?g. }
- W2891802653 endingPage "9" @default.
- W2891802653 startingPage "1" @default.
- W2891802653 abstract "Chrysanthemum stunt viroid (CSVd) and chrysanthemum chlorotic mottle viroid (CChMVd) are the two viroids most frequently infecting chrysanthemum. This study attempted to cryopreserve in vitro shoot tips of Chrysanthemum morifolium ‘Piato’ infected with high or low titers of CSVd, and co-infected with CSVd and CChMVd. By optimizing several key factors including the time and light regimes during cold-hardening of stock shoots, and the size of shoot tips, an encapsulation-vitrification procedure was established for cryopreservation of viroid-infected shoot tips. Viroid-infected stock shoots were cold-hardened in vitro at 4 °C in a 16-h photoperiod for 6 weeks. Shoot tips (1.5 mm in size) containing 3-4 LPs were excised from the cold-hardened stock shoots and subjected to encapsulation-vitrification cryopreservation. With this protocol, about 65%, 45% and 42% of shoot regrowth levels were obtained in cryopreserved shoot tips derived from in vitro stock shoots infected with low or high titers of CSVd, and co-infected with CSVd and CChMVd, respectively. All regenerants from cryopreservation maintained their viroid-infected status, identical to those of their in vitro stock shoots. Histological observations and in situ hybridization elucidated why cryopreservation could maintain viroids in cryopreserved shoot tips. Cryopreservation of viroid-infected plant materials has potential applications to all types of viroid-related basic and applied studies." @default.
- W2891802653 created "2018-09-27" @default.
- W2891802653 creator A5001295469 @default.
- W2891802653 creator A5004839646 @default.
- W2891802653 creator A5026737772 @default.
- W2891802653 creator A5031621722 @default.
- W2891802653 creator A5034762138 @default.
- W2891802653 creator A5084905184 @default.
- W2891802653 date "2019-01-01" @default.
- W2891802653 modified "2023-09-27" @default.
- W2891802653 title "Cryopreservation of viroid-infected chrysanthemum shoot tips" @default.
- W2891802653 cites W1173529113 @default.
- W2891802653 cites W1840760761 @default.
- W2891802653 cites W1967542940 @default.
- W2891802653 cites W1969155516 @default.
- W2891802653 cites W1969343384 @default.
- W2891802653 cites W1972591849 @default.
- W2891802653 cites W1977827182 @default.
- W2891802653 cites W1990917592 @default.
- W2891802653 cites W1991122683 @default.
- W2891802653 cites W1995811423 @default.
- W2891802653 cites W2011412767 @default.
- W2891802653 cites W2018575993 @default.
- W2891802653 cites W2019250968 @default.
- W2891802653 cites W2024556021 @default.
- W2891802653 cites W2025836077 @default.
- W2891802653 cites W2030342240 @default.
- W2891802653 cites W2031419077 @default.
- W2891802653 cites W2035515051 @default.
- W2891802653 cites W2042751765 @default.
- W2891802653 cites W2044860740 @default.
- W2891802653 cites W2045232941 @default.
- W2891802653 cites W2047510441 @default.
- W2891802653 cites W2052092292 @default.
- W2891802653 cites W2054746091 @default.
- W2891802653 cites W2057558123 @default.
- W2891802653 cites W2059720373 @default.
- W2891802653 cites W2065278152 @default.
- W2891802653 cites W2075094995 @default.
- W2891802653 cites W2080137816 @default.
- W2891802653 cites W2087704566 @default.
- W2891802653 cites W2096249174 @default.
- W2891802653 cites W2113067345 @default.
- W2891802653 cites W2122677847 @default.
- W2891802653 cites W2136540077 @default.
- W2891802653 cites W2188045924 @default.
- W2891802653 cites W2215189213 @default.
- W2891802653 cites W2316524231 @default.
- W2891802653 cites W2328014250 @default.
- W2891802653 cites W234815448 @default.
- W2891802653 cites W2471063053 @default.
- W2891802653 cites W2549657613 @default.
- W2891802653 cites W258760677 @default.
- W2891802653 cites W2589287154 @default.
- W2891802653 cites W2593561482 @default.
- W2891802653 cites W2642851852 @default.
- W2891802653 cites W2773195234 @default.
- W2891802653 cites W291048730 @default.
- W2891802653 doi "https://doi.org/10.1016/j.scienta.2018.09.004" @default.
- W2891802653 hasPublicationYear "2019" @default.
- W2891802653 type Work @default.
- W2891802653 sameAs 2891802653 @default.
- W2891802653 citedByCount "9" @default.
- W2891802653 countsByYear W28918026532018 @default.
- W2891802653 countsByYear W28918026532019 @default.
- W2891802653 countsByYear W28918026532020 @default.
- W2891802653 countsByYear W28918026532022 @default.
- W2891802653 countsByYear W28918026532023 @default.
- W2891802653 crossrefType "journal-article" @default.
- W2891802653 hasAuthorship W2891802653A5001295469 @default.
- W2891802653 hasAuthorship W2891802653A5004839646 @default.
- W2891802653 hasAuthorship W2891802653A5026737772 @default.
- W2891802653 hasAuthorship W2891802653A5031621722 @default.
- W2891802653 hasAuthorship W2891802653A5034762138 @default.
- W2891802653 hasAuthorship W2891802653A5084905184 @default.
- W2891802653 hasConcept C109110057 @default.
- W2891802653 hasConcept C144027150 @default.
- W2891802653 hasConcept C151730666 @default.
- W2891802653 hasConcept C159047783 @default.
- W2891802653 hasConcept C16685009 @default.
- W2891802653 hasConcept C179933525 @default.
- W2891802653 hasConcept C183282558 @default.
- W2891802653 hasConcept C196843134 @default.
- W2891802653 hasConcept C21410773 @default.
- W2891802653 hasConcept C2522874641 @default.
- W2891802653 hasConcept C2778022349 @default.
- W2891802653 hasConcept C2778874994 @default.
- W2891802653 hasConcept C2780781446 @default.
- W2891802653 hasConcept C54355233 @default.
- W2891802653 hasConcept C59822182 @default.
- W2891802653 hasConcept C67236022 @default.
- W2891802653 hasConcept C71924100 @default.
- W2891802653 hasConcept C86803240 @default.
- W2891802653 hasConceptScore W2891802653C109110057 @default.
- W2891802653 hasConceptScore W2891802653C144027150 @default.
- W2891802653 hasConceptScore W2891802653C151730666 @default.
- W2891802653 hasConceptScore W2891802653C159047783 @default.
- W2891802653 hasConceptScore W2891802653C16685009 @default.