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- W2891845439 abstract "Abstract Immunofluorescence staining is the key technique for visualizing organization of endogenous cellular structures in single cells. Labeling and imaging of blood stage Plasmodium falciparum has always been challenging since it is a small intracellular parasite. The gold standard for parasite immunofluorescence is fixation in suspension with addition of minute amounts of glutaraldehyde to the paraformaldehyde-based solution. While this maintains red blood cell integrity, it has been postulated that antigenicity of the parasite proteins was, if at all, only slightly reduced. Here we show the deleterious effect that even these small quantities of glutaraldehyde can have on immunofluorescence staining quality and present an alternative cell seeding protocol that allows fixation with only paraformaldehyde. The highly improved signal intensity and staining efficiency enabled us to carry out RescueSTED nanoscopy on microtubules and nuclear pores and describe their organization in greater detail throughout the blood stage cycle. Highlights - Omitting glutaraldehyde from fixative improves immunofluorescence staining - STED nanoscopy is readily applicable to infected red blood cells - Intranuclear microtubules are nucleated from distinct sites" @default.
- W2891845439 created "2018-09-27" @default.
- W2891845439 creator A5051298879 @default.
- W2891845439 creator A5058384591 @default.
- W2891845439 date "2018-09-14" @default.
- W2891845439 modified "2023-09-25" @default.
- W2891845439 title "Immunofluorescence staining protocol for STED nanoscopy of Plasmodium-infected red blood cells" @default.
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- W2891845439 doi "https://doi.org/10.1101/416776" @default.
- W2891845439 hasPublicationYear "2018" @default.
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