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- W2891878357 abstract "Summary Inorganic polyphosphate (polyP) is a polymer of three to hundreds of phosphate units bound by high‐energy phosphoanhydride bonds and present from bacteria to humans. Most polyP in trypanosomatids is concentrated in acidocalcisomes, acidic calcium stores that possess a number of pumps, exchangers, and channels, and are important for their survival. In this work, using polyP as bait we identified > 25 putative protein targets in cell lysates of both Trypanosoma cruzi and Trypanosoma brucei . Gene ontology analysis of the binding partners found a significant over‐representation of nucleolar and glycosomal proteins. Using the polyphosphate‐binding domain (PPBD) of Escherichia coli exopolyphosphatase (PPX), we localized long‐chain polyP to the nucleoli and glycosomes of trypanosomes. A competitive assay based on the pre‐incubation of PPBD with exogenous polyP and subsequent immunofluorescence assay of procyclic forms (PCF) of T. brucei showed polyP concentration‐dependent and chain length‐dependent decrease in the fluorescence signal. Subcellular fractionation experiments confirmed the presence of polyP in glycosomes of T. brucei PCF. Targeting of yeast PPX to the glycosomes of PCF resulted in polyP hydrolysis, alteration in their glycolytic flux and increase in their susceptibility to oxidative stress." @default.
- W2891878357 created "2018-09-27" @default.
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- W2891878357 creator A5080935177 @default.
- W2891878357 date "2018-10-18" @default.
- W2891878357 modified "2023-10-15" @default.
- W2891878357 title "Inorganic polyphosphate interacts with nucleolar and glycosomal proteins in trypanosomatids" @default.
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- W2891878357 doi "https://doi.org/10.1111/mmi.14131" @default.
- W2891878357 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6281790" @default.
- W2891878357 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30230089" @default.
- W2891878357 hasPublicationYear "2018" @default.
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