Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891892632> ?p ?o ?g. }
- W2891892632 abstract "ABSTRACT In laboratory strains of zebrafish, sex determination occurs in the absence of a typical sex chromosome and it is not known what regulates the proportion of animals that develop as male or female. Many sex determination and differentiation genes that act downstream of a sex chromosome are well conserved among vertebrates, but studies that test their contribution to this process have mostly been limited to mammalian models. In mammals, WNT4 is a signaling ligand that is essential for ovary and Müllerian duct development, where it function, in part, to antagonize the male-promoting FGF9 signal. Wnt4 is highly conserved in non-mammalian vertebrates, but it is not known if Wnt4 plays a role in sex determination and/or the differentiation of sex organs outside of mammals. This is an especially interesting question in teleost, such as zebrafish, because they lack an Fgf9 ortholog. Here we show that wnt4a is the ortholog of mammalian Wnt4, and that wnt4b was present in the last common ancestor of humans and zebrafish, but was lost in mammals. We found that wnt4a is expressed in the somatic cells of juvenile gonads during the time sex determination likely occurs. We show that wnt4a loss-of-function mutants develop predominantly as males and conclude that wnt4a activity promotes female sex determination in zebrafish. Additionally, both male and female wnt4a mutants are sterile because their reproductive ducts do not connect to the vent, where wnt4a is normally expressed. Yet when dissected from homozygous wnt4a mutant gonads, both sperm and eggs can produce fertile offspring. Together these results strongly argue that Wnt4a is a conserved regulator of female sex determination and reproductive duct development in non-mammalian vertebrates. SUMMARY Wnt4 is a key regulator of ovary development in mammals, but it is not known if it plays a similar role in other vertebrates. Here we show that zebrafish wnt4a is the ortholog of mammalian Wnt4 . We show that wnt4a is expressed in zebrafish somatic gonad cells during the time sex determination likely occurs. Through analysis of wnt4a mutants, we show that Wnt4a promotes female sex determination and the development of the male and female reproductive. We conclude that Wnt4/Wnt4a is likely a conserved regulator of ovarian and reproductive duct development in all vertebrates" @default.
- W2891892632 created "2018-09-27" @default.
- W2891892632 creator A5001512160 @default.
- W2891892632 creator A5008936142 @default.
- W2891892632 creator A5016003096 @default.
- W2891892632 creator A5026801171 @default.
- W2891892632 creator A5069087137 @default.
- W2891892632 creator A5082715439 @default.
- W2891892632 date "2018-09-19" @default.
- W2891892632 modified "2023-10-16" @default.
- W2891892632 title "wnt4a promotes female development and reproductive duct elongation in zebrafish" @default.
- W2891892632 cites W1498970130 @default.
- W2891892632 cites W1634167063 @default.
- W2891892632 cites W1974094726 @default.
- W2891892632 cites W1977168957 @default.
- W2891892632 cites W1980605506 @default.
- W2891892632 cites W1981239255 @default.
- W2891892632 cites W1992921729 @default.
- W2891892632 cites W1995827355 @default.
- W2891892632 cites W2004609419 @default.
- W2891892632 cites W2005228725 @default.
- W2891892632 cites W2013052396 @default.
- W2891892632 cites W2013554143 @default.
- W2891892632 cites W2015499825 @default.
- W2891892632 cites W2025874719 @default.
- W2891892632 cites W2027255572 @default.
- W2891892632 cites W2029634472 @default.
- W2891892632 cites W2040001653 @default.
- W2891892632 cites W2043458144 @default.
- W2891892632 cites W2047405437 @default.
- W2891892632 cites W2051401308 @default.
- W2891892632 cites W2062003019 @default.
- W2891892632 cites W2064912476 @default.
- W2891892632 cites W2068166332 @default.
- W2891892632 cites W2078716122 @default.
- W2891892632 cites W2083035724 @default.
- W2891892632 cites W2097686052 @default.
- W2891892632 cites W2106235028 @default.
- W2891892632 cites W2109077589 @default.
- W2891892632 cites W2113714901 @default.
- W2891892632 cites W2117919012 @default.
- W2891892632 cites W2120960764 @default.
- W2891892632 cites W2132023322 @default.
- W2891892632 cites W2140861044 @default.
- W2891892632 cites W2143482057 @default.
- W2891892632 cites W2154969423 @default.
- W2891892632 cites W2155062762 @default.
- W2891892632 cites W2158701974 @default.
- W2891892632 cites W2162581076 @default.
- W2891892632 cites W2221177324 @default.
- W2891892632 cites W2260083591 @default.
- W2891892632 cites W2296550515 @default.
- W2891892632 cites W2421858102 @default.
- W2891892632 cites W2521989110 @default.
- W2891892632 cites W2560574425 @default.
- W2891892632 cites W2621361823 @default.
- W2891892632 cites W2752062112 @default.
- W2891892632 cites W2756230282 @default.
- W2891892632 cites W4255616152 @default.
- W2891892632 doi "https://doi.org/10.1101/421362" @default.
- W2891892632 hasPublicationYear "2018" @default.
- W2891892632 type Work @default.
- W2891892632 sameAs 2891892632 @default.
- W2891892632 citedByCount "0" @default.
- W2891892632 crossrefType "posted-content" @default.
- W2891892632 hasAuthorship W2891892632A5001512160 @default.
- W2891892632 hasAuthorship W2891892632A5008936142 @default.
- W2891892632 hasAuthorship W2891892632A5016003096 @default.
- W2891892632 hasAuthorship W2891892632A5026801171 @default.
- W2891892632 hasAuthorship W2891892632A5069087137 @default.
- W2891892632 hasAuthorship W2891892632A5082715439 @default.
- W2891892632 hasBestOaLocation W28918926321 @default.
- W2891892632 hasConcept C104317684 @default.
- W2891892632 hasConcept C134305767 @default.
- W2891892632 hasConcept C137620995 @default.
- W2891892632 hasConcept C143065580 @default.
- W2891892632 hasConcept C2776878037 @default.
- W2891892632 hasConcept C2778324911 @default.
- W2891892632 hasConcept C2779066055 @default.
- W2891892632 hasConcept C2780384893 @default.
- W2891892632 hasConcept C2781430442 @default.
- W2891892632 hasConcept C2909959250 @default.
- W2891892632 hasConcept C54355233 @default.
- W2891892632 hasConcept C63405245 @default.
- W2891892632 hasConcept C86803240 @default.
- W2891892632 hasConcept C95444343 @default.
- W2891892632 hasConceptScore W2891892632C104317684 @default.
- W2891892632 hasConceptScore W2891892632C134305767 @default.
- W2891892632 hasConceptScore W2891892632C137620995 @default.
- W2891892632 hasConceptScore W2891892632C143065580 @default.
- W2891892632 hasConceptScore W2891892632C2776878037 @default.
- W2891892632 hasConceptScore W2891892632C2778324911 @default.
- W2891892632 hasConceptScore W2891892632C2779066055 @default.
- W2891892632 hasConceptScore W2891892632C2780384893 @default.
- W2891892632 hasConceptScore W2891892632C2781430442 @default.
- W2891892632 hasConceptScore W2891892632C2909959250 @default.
- W2891892632 hasConceptScore W2891892632C54355233 @default.
- W2891892632 hasConceptScore W2891892632C63405245 @default.
- W2891892632 hasConceptScore W2891892632C86803240 @default.
- W2891892632 hasConceptScore W2891892632C95444343 @default.