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- W2891903107 abstract "Background: Magnetic bead immunocapture-LC–MS has been widely used for bioanalysis of biotherapeutic proteins. However, magnetic beads are difficult to be fully automated and more costly than ELISA plates. Aim: Develop an ELISA–LC–MS hybrid assay as an alternate platform. Results: Among seven ELISA plates tested, Pierce streptavidin plates, which did not require time-consuming capture antibody precoating steps, provided the best sensitivity and assay dynamic range (5–2500 ng/ml or 10–5000 ng/ml), similar to magnetic bead immunocapture–LC–MS assay and better than an ELISA (50–500 ng/ml). The entire procedures could be fully automated using a liquid handling system. Conclusion: This study demonstrates that ELISA–LC–MS hybrid approach using streptavidin plates represents a promising platform for bioanalysis of biotherapeutics." @default.
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- W2891903107 date "2018-09-01" @default.
- W2891903107 modified "2023-09-23" @default.
- W2891903107 title "Development of an ELISA–LC–MS hybrid assay for quantification of biotherapeutics" @default.
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- W2891903107 doi "https://doi.org/10.4155/bio-2018-0082" @default.
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