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- W2891915000 abstract "Store-operated Ca2+ entry (SOCE) pathway plays important roles in many cellular processes, which is largely studied by using fluorescent Ca2+ indicator, Fura-2. Extracellular Mn2+ is able to cross the plasma membrane through SOCE and quenches the fluorescence signals from Fura-2. Thus, the fluorescence quenching rate by Mn2+ composes a convenient assay to monitor the extent of SOCE. This chapter describes an experimental method of Mn2+ quenching assay for both cultured esophageal epithelial and skeletal muscle cells. It also explains how to perform a quantitative analysis of graded SOCE." @default.
- W2891915000 created "2018-09-27" @default.
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- W2891915000 date "2018-01-01" @default.
- W2891915000 modified "2023-10-18" @default.
- W2891915000 title "Mn2+ Quenching Assay for Store-Operated Calcium Entry" @default.
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- W2891915000 doi "https://doi.org/10.1007/978-1-4939-8704-7_4" @default.
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