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- W2891917662 endingPage "e1007284" @default.
- W2891917662 startingPage "e1007284" @default.
- W2891917662 abstract "Hepatitis C virus (HCV) infection constitutes a significant health burden worldwide, because it is a major etiologic agent of chronic liver disease, cirrhosis and hepatocellular carcinoma. HCV replication cycle is closely tied to lipid metabolism and infection by this virus causes profound changes in host lipid homeostasis. We focused our attention on a phosphatidate phosphate (PAP) enzyme family (the lipin family), which mediate the conversion of phosphatidate to diacylglycerol in the cytoplasm, playing a key role in triglyceride biosynthesis and in phospholipid homeostasis. Lipins may also translocate to the nucleus to act as transcriptional regulators of genes involved in lipid metabolism. The best-characterized member of this family is lipin1, which cooperates with lipin2 to maintain glycerophospholipid homeostasis in the liver. Lipin1-deficient cell lines were generated by RNAi to study the role of this protein in different steps of HCV replication cycle. Using surrogate models that recapitulate different aspects of HCV infection, we concluded that lipin1 is rate limiting for the generation of functional replicase complexes, in a step downstream primary translation that leads to early HCV RNA replication. Infection studies in lipin1-deficient cells overexpressing wild type or phosphatase-defective lipin1 proteins suggest that lipin1 phosphatase activity is required to support HCV infection. Finally, ultrastructural and biochemical analyses in replication-independent models suggest that lipin1 may facilitate the generation of the membranous compartment that contains functional HCV replicase complexes." @default.
- W2891917662 created "2018-09-27" @default.
- W2891917662 creator A5003859488 @default.
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- W2891917662 creator A5062003448 @default.
- W2891917662 creator A5066021450 @default.
- W2891917662 creator A5081961105 @default.
- W2891917662 date "2018-09-18" @default.
- W2891917662 modified "2023-10-18" @default.
- W2891917662 title "Host phosphatidic acid phosphatase lipin1 is rate limiting for functional hepatitis C virus replicase complex formation" @default.
- W2891917662 cites W1523366628 @default.
- W2891917662 cites W1535922733 @default.
- W2891917662 cites W1715619413 @default.
- W2891917662 cites W1892311206 @default.
- W2891917662 cites W1966153332 @default.
- W2891917662 cites W1969699638 @default.
- W2891917662 cites W1977546905 @default.
- W2891917662 cites W1979110414 @default.
- W2891917662 cites W1980805512 @default.
- W2891917662 cites W1984040540 @default.
- W2891917662 cites W1986944906 @default.
- W2891917662 cites W1987002413 @default.
- W2891917662 cites W1987984630 @default.
- W2891917662 cites W1989571181 @default.
- W2891917662 cites W1992980640 @default.
- W2891917662 cites W1994093923 @default.
- W2891917662 cites W1996803605 @default.
- W2891917662 cites W1997385930 @default.
- W2891917662 cites W1998373476 @default.
- W2891917662 cites W1998384937 @default.
- W2891917662 cites W1998682759 @default.
- W2891917662 cites W2000434613 @default.
- W2891917662 cites W2003579943 @default.
- W2891917662 cites W2004968462 @default.
- W2891917662 cites W2005790383 @default.
- W2891917662 cites W2011677146 @default.
- W2891917662 cites W2014290325 @default.
- W2891917662 cites W2017221029 @default.
- W2891917662 cites W2020725477 @default.
- W2891917662 cites W2021993761 @default.
- W2891917662 cites W2022687771 @default.
- W2891917662 cites W2040154453 @default.
- W2891917662 cites W2044193462 @default.
- W2891917662 cites W2045104704 @default.
- W2891917662 cites W2046984465 @default.
- W2891917662 cites W2052660293 @default.
- W2891917662 cites W2055005801 @default.
- W2891917662 cites W2056724322 @default.
- W2891917662 cites W2059670505 @default.
- W2891917662 cites W2063874036 @default.
- W2891917662 cites W2066140350 @default.
- W2891917662 cites W2066207556 @default.
- W2891917662 cites W2067299494 @default.
- W2891917662 cites W2068602754 @default.
- W2891917662 cites W2069633173 @default.
- W2891917662 cites W2075833662 @default.
- W2891917662 cites W2079599967 @default.
- W2891917662 cites W2081171941 @default.
- W2891917662 cites W2083117917 @default.
- W2891917662 cites W2083901349 @default.
- W2891917662 cites W2084084147 @default.
- W2891917662 cites W2084967585 @default.
- W2891917662 cites W2088337621 @default.
- W2891917662 cites W2088621072 @default.
- W2891917662 cites W2089441009 @default.
- W2891917662 cites W2096963938 @default.
- W2891917662 cites W2099540110 @default.
- W2891917662 cites W2104356883 @default.
- W2891917662 cites W2108388328 @default.
- W2891917662 cites W2110525565 @default.
- W2891917662 cites W2110671968 @default.
- W2891917662 cites W2110842901 @default.
- W2891917662 cites W2112825470 @default.
- W2891917662 cites W2113689677 @default.
- W2891917662 cites W2121863870 @default.
- W2891917662 cites W2122170727 @default.
- W2891917662 cites W2123724780 @default.
- W2891917662 cites W2124733101 @default.
- W2891917662 cites W2127274349 @default.
- W2891917662 cites W2127481048 @default.
- W2891917662 cites W2129069648 @default.
- W2891917662 cites W2131605289 @default.
- W2891917662 cites W2131970062 @default.
- W2891917662 cites W2132468746 @default.
- W2891917662 cites W2134812217 @default.
- W2891917662 cites W2138488044 @default.
- W2891917662 cites W2144235090 @default.
- W2891917662 cites W2144246402 @default.
- W2891917662 cites W2150368001 @default.
- W2891917662 cites W2154171026 @default.
- W2891917662 cites W2156798015 @default.
- W2891917662 cites W2160662703 @default.
- W2891917662 cites W2162645720 @default.
- W2891917662 cites W2164249299 @default.