Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891924821> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2891924821 endingPage "31" @default.
- W2891924821 startingPage "17" @default.
- W2891924821 abstract "Membrane proteins play important roles in many biological processes and are a major drug target. However, only a limited number of structures of eukaryotic membrane proteins have been determined so far. Besides the challenges in crystallizing these proteins, one of the main bottlenecks in structure determination is their recombinant expression. The mammalian HEK293 cell line provides a natural environment for expression of eukaryotic membrane proteins but optimization of transfection and cultivation time is often necessary to yield amounts of protein suitable for structural studies.Here we describe a detailed protocol for expression and purification of membrane proteins from HEK293 cells with an example of the human peptide transporter, PepT2. In the first part, we focus on the expression optimization by changing transfection protocol and cultivation time. In the second part, we describe a robust protocol for large-scale expression and purification of membrane proteins based on affinity chromatography and gel filtration." @default.
- W2891924821 created "2018-09-27" @default.
- W2891924821 creator A5004695848 @default.
- W2891924821 creator A5054655773 @default.
- W2891924821 creator A5060449678 @default.
- W2891924821 date "2018-01-01" @default.
- W2891924821 modified "2023-09-30" @default.
- W2891924821 title "Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells" @default.
- W2891924821 cites W1227091703 @default.
- W2891924821 cites W1602663908 @default.
- W2891924821 cites W1997468757 @default.
- W2891924821 cites W2016384668 @default.
- W2891924821 cites W2052407981 @default.
- W2891924821 cites W2071619296 @default.
- W2891924821 cites W2093599867 @default.
- W2891924821 cites W2117471612 @default.
- W2891924821 cites W2118461522 @default.
- W2891924821 cites W2132820240 @default.
- W2891924821 cites W2162518738 @default.
- W2891924821 cites W2164953211 @default.
- W2891924821 cites W2485216659 @default.
- W2891924821 doi "https://doi.org/10.1007/978-1-4939-8730-6_2" @default.
- W2891924821 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30242677" @default.
- W2891924821 hasPublicationYear "2018" @default.
- W2891924821 type Work @default.
- W2891924821 sameAs 2891924821 @default.
- W2891924821 citedByCount "8" @default.
- W2891924821 countsByYear W28919248212020 @default.
- W2891924821 countsByYear W28919248212021 @default.
- W2891924821 countsByYear W28919248212022 @default.
- W2891924821 countsByYear W28919248212023 @default.
- W2891924821 crossrefType "book-chapter" @default.
- W2891924821 hasAuthorship W2891924821A5004695848 @default.
- W2891924821 hasAuthorship W2891924821A5054655773 @default.
- W2891924821 hasAuthorship W2891924821A5060449678 @default.
- W2891924821 hasConcept C104317684 @default.
- W2891924821 hasConcept C123894998 @default.
- W2891924821 hasConcept C142613039 @default.
- W2891924821 hasConcept C144647389 @default.
- W2891924821 hasConcept C174510640 @default.
- W2891924821 hasConcept C185592680 @default.
- W2891924821 hasConcept C40767141 @default.
- W2891924821 hasConcept C41625074 @default.
- W2891924821 hasConcept C54009773 @default.
- W2891924821 hasConcept C55493867 @default.
- W2891924821 hasConcept C86803240 @default.
- W2891924821 hasConcept C8985478 @default.
- W2891924821 hasConcept C95444343 @default.
- W2891924821 hasConcept C97037327 @default.
- W2891924821 hasConceptScore W2891924821C104317684 @default.
- W2891924821 hasConceptScore W2891924821C123894998 @default.
- W2891924821 hasConceptScore W2891924821C142613039 @default.
- W2891924821 hasConceptScore W2891924821C144647389 @default.
- W2891924821 hasConceptScore W2891924821C174510640 @default.
- W2891924821 hasConceptScore W2891924821C185592680 @default.
- W2891924821 hasConceptScore W2891924821C40767141 @default.
- W2891924821 hasConceptScore W2891924821C41625074 @default.
- W2891924821 hasConceptScore W2891924821C54009773 @default.
- W2891924821 hasConceptScore W2891924821C55493867 @default.
- W2891924821 hasConceptScore W2891924821C86803240 @default.
- W2891924821 hasConceptScore W2891924821C8985478 @default.
- W2891924821 hasConceptScore W2891924821C95444343 @default.
- W2891924821 hasConceptScore W2891924821C97037327 @default.
- W2891924821 hasLocation W28919248211 @default.
- W2891924821 hasLocation W28919248212 @default.
- W2891924821 hasOpenAccess W2891924821 @default.
- W2891924821 hasPrimaryLocation W28919248211 @default.
- W2891924821 hasRelatedWork W1967639197 @default.
- W2891924821 hasRelatedWork W2095924822 @default.
- W2891924821 hasRelatedWork W2227157292 @default.
- W2891924821 hasRelatedWork W2369322244 @default.
- W2891924821 hasRelatedWork W2373087578 @default.
- W2891924821 hasRelatedWork W2379145368 @default.
- W2891924821 hasRelatedWork W2384543036 @default.
- W2891924821 hasRelatedWork W2386445198 @default.
- W2891924821 hasRelatedWork W2391890413 @default.
- W2891924821 hasRelatedWork W2900537141 @default.
- W2891924821 isParatext "false" @default.
- W2891924821 isRetracted "false" @default.
- W2891924821 magId "2891924821" @default.
- W2891924821 workType "book-chapter" @default.