Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891925472> ?p ?o ?g. }
- W2891925472 endingPage "216" @default.
- W2891925472 startingPage "211" @default.
- W2891925472 abstract "Exosomes as cell-derived vesicles have the potential to be novel biomarkers for noninvasive diagnosis of cancers. However, cost-effective detection of exosomes in routine clinical settings is still challenging. Herein, we present a ZnO nanowires coated three-dimensional (3D) scaffold chip device for effective immunocapture and classically visible and colorimetric detection of exosomes. The chip device is composed of 3D polydimethylsiloxane (PDMS) scaffold skeleton covered by free-standing ZnO nanowire array. The interconnected micropores of 3D scaffold induces the fluid flow with chaotic or vortex feature, and ZnO nanowire array provides large surface area for immobilization of exosome specific antibody as well as size exclusion-like effect for retaining exosomes. These synergistically and significantly enhance the capture of exosomes at a high flow rate. The captured exosomes are detected by horseradish peroxidase (HRP) labeled antibody which can initiate 3,3',5,5'-tetramethylbenzidine (TMB)-based colorimetric sensing. The quantitative readout of exosomes is easily accomplished by UV-vis spectrometry or microplate reader with a linear range of 2.2 × 105 to 2.4 × 107 particles/μL and a minimal detectable concentration of 2.2 × 104 particles/μL. This chip device was applicable to clinical samples where cancer patients demonstrate statistically significant increase in exosomes compared with healthy individuals. Thus, our chip device is cost-effective and easy-to-use, facilitating visible and colorimetric assay with high sensitivity toward clinical applications." @default.
- W2891925472 created "2018-09-27" @default.
- W2891925472 creator A5032683909 @default.
- W2891925472 creator A5035667799 @default.
- W2891925472 creator A5037458498 @default.
- W2891925472 creator A5051736674 @default.
- W2891925472 creator A5070591558 @default.
- W2891925472 creator A5073580267 @default.
- W2891925472 creator A5076712472 @default.
- W2891925472 creator A5087631945 @default.
- W2891925472 date "2018-12-01" @default.
- W2891925472 modified "2023-10-15" @default.
- W2891925472 title "Detection of exosomes by ZnO nanowires coated three-dimensional scaffold chip device" @default.
- W2891925472 cites W1500925570 @default.
- W2891925472 cites W1762620449 @default.
- W2891925472 cites W1839188703 @default.
- W2891925472 cites W1877686344 @default.
- W2891925472 cites W1937665132 @default.
- W2891925472 cites W1950118853 @default.
- W2891925472 cites W1971364766 @default.
- W2891925472 cites W1972934647 @default.
- W2891925472 cites W1987409881 @default.
- W2891925472 cites W2004874639 @default.
- W2891925472 cites W2015222608 @default.
- W2891925472 cites W2022805251 @default.
- W2891925472 cites W2031454914 @default.
- W2891925472 cites W2033598310 @default.
- W2891925472 cites W2042445350 @default.
- W2891925472 cites W2051678321 @default.
- W2891925472 cites W2097343453 @default.
- W2891925472 cites W2097774418 @default.
- W2891925472 cites W2100886586 @default.
- W2891925472 cites W2105726520 @default.
- W2891925472 cites W2117909529 @default.
- W2891925472 cites W2121354606 @default.
- W2891925472 cites W2138700918 @default.
- W2891925472 cites W2160436654 @default.
- W2891925472 cites W2169336864 @default.
- W2891925472 cites W2296538017 @default.
- W2891925472 cites W2321130266 @default.
- W2891925472 cites W2460125764 @default.
- W2891925472 cites W2477339980 @default.
- W2891925472 cites W2560669180 @default.
- W2891925472 cites W2568802331 @default.
- W2891925472 cites W2583560695 @default.
- W2891925472 cites W2607716704 @default.
- W2891925472 cites W2729760051 @default.
- W2891925472 cites W2741701179 @default.
- W2891925472 cites W2745201210 @default.
- W2891925472 cites W2774100359 @default.
- W2891925472 cites W2783823932 @default.
- W2891925472 cites W654621879 @default.
- W2891925472 doi "https://doi.org/10.1016/j.bios.2018.09.033" @default.
- W2891925472 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30265971" @default.
- W2891925472 hasPublicationYear "2018" @default.
- W2891925472 type Work @default.
- W2891925472 sameAs 2891925472 @default.
- W2891925472 citedByCount "93" @default.
- W2891925472 countsByYear W28919254722019 @default.
- W2891925472 countsByYear W28919254722020 @default.
- W2891925472 countsByYear W28919254722021 @default.
- W2891925472 countsByYear W28919254722022 @default.
- W2891925472 countsByYear W28919254722023 @default.
- W2891925472 crossrefType "journal-article" @default.
- W2891925472 hasAuthorship W2891925472A5032683909 @default.
- W2891925472 hasAuthorship W2891925472A5035667799 @default.
- W2891925472 hasAuthorship W2891925472A5037458498 @default.
- W2891925472 hasAuthorship W2891925472A5051736674 @default.
- W2891925472 hasAuthorship W2891925472A5070591558 @default.
- W2891925472 hasAuthorship W2891925472A5073580267 @default.
- W2891925472 hasAuthorship W2891925472A5076712472 @default.
- W2891925472 hasAuthorship W2891925472A5087631945 @default.
- W2891925472 hasConcept C104317684 @default.
- W2891925472 hasConcept C136229726 @default.
- W2891925472 hasConcept C145059251 @default.
- W2891925472 hasConcept C155672457 @default.
- W2891925472 hasConcept C171250308 @default.
- W2891925472 hasConcept C181199279 @default.
- W2891925472 hasConcept C185592680 @default.
- W2891925472 hasConcept C192562407 @default.
- W2891925472 hasConcept C20518536 @default.
- W2891925472 hasConcept C2777676563 @default.
- W2891925472 hasConcept C2779849746 @default.
- W2891925472 hasConcept C2781261824 @default.
- W2891925472 hasConcept C55493867 @default.
- W2891925472 hasConcept C71924100 @default.
- W2891925472 hasConcept C74214498 @default.
- W2891925472 hasConcept C8673954 @default.
- W2891925472 hasConcept C89429830 @default.
- W2891925472 hasConceptScore W2891925472C104317684 @default.
- W2891925472 hasConceptScore W2891925472C136229726 @default.
- W2891925472 hasConceptScore W2891925472C145059251 @default.
- W2891925472 hasConceptScore W2891925472C155672457 @default.
- W2891925472 hasConceptScore W2891925472C171250308 @default.
- W2891925472 hasConceptScore W2891925472C181199279 @default.
- W2891925472 hasConceptScore W2891925472C185592680 @default.
- W2891925472 hasConceptScore W2891925472C192562407 @default.
- W2891925472 hasConceptScore W2891925472C20518536 @default.