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• W2891933006 abstract "Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affinity binders from libraries of folded proteins. It works entirely in vitro, and this has two important consequences. First, since no transformation of any cells is required, libraries with much greater diversity can be handled than with most other techniques. Second, since a library does not have to be cloned and transformed, it is very convenient to introduce random errors in the library by PCR-based methods and select improved binders. Thus, a true directed evolution, an iteration between randomization and selection over several generations, can be conveniently carried out, e.g., for affinity maturation, either on a given clone or on the whole library. Ribosome display has been successfully applied to antibody single-chain Fv fragments (scFv), which can be selected not only for specificity but also for stability and catalytic activity. High-affinity binders with new target specificity can be obtained from highly diverse libraries in only a few selection rounds. In this protocol, the selection from the library and the process of affinity maturation and off-rate selection are explained in detail." @default.
• W2891933006 created "2018-09-27" @default.
• W2891933006 creator A5016483675 @default.
• W2891933006 creator A5083658123 @default.
• W2891933006 date "2018-01-01" @default.
• W2891933006 modified "2023-10-02" @default.
• W2891933006 title "Rapid Selection of High-Affinity Antibody scFv Fragments Using Ribosome Display" @default.
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• W2891933006 doi "https://doi.org/10.1007/978-1-4939-8648-4_13" @default.
• W2891933006 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30196501" @default.
• W2891933006 hasPublicationYear "2018" @default.
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