Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891933855> ?p ?o ?g. }
- W2891933855 endingPage "76" @default.
- W2891933855 startingPage "68" @default.
- W2891933855 abstract "Although the cross-kingdom transfer of vegetable miRNAs (miRNAs) in mammalian species, including humans, is still controversial, recent studies have rejected this theory. Based on these recent studies, we hypothesized that artichoke-derived miRNAs (cca-miRNAs) are not adsorbed into human intestinal cells after cooking and in vitro digestion. In order to test this hypothesis, we evaluated miRNA (cca-miRNAs) in the edible part of globe artichokes (head portion), after cooking and digestion by an in vitro digestion system. The cca-miRNA levels were analyzed by real-time PCR (RT-qPCR), and those that withstood cooking and digestion conditions were further analyzed for their bioavailability using an in vitro system (Caco-2/TC7 cell clone). We detected 20 cca-miRNAs after cooking, 5 of which were statistically down-regulated in comparison with uncooked samples. Only 4 cca-miRNAs were found after in vitro digestion. By using scanning electron microscopy (SEM), we also evaluated the extracellular vesicles (EVs) in homogenized artichoke as possible miRNA transporters. However, approximately 81% were degraded after cooking, while the remaining EVs had changed shape from round to elliptical. Finally, we detected no cell-free cca-miRNAs, miRNAs bound to protein complex, and no cca-miRNAs encapsulated in EVs inside Caco-2 cells or in basolateral medium after bioavailability experiments. In conclusion, the data from the present study agrees with recent findings that the human small intestine does not uptake dietary miRNAs from raw or cooked artichoke heads." @default.
- W2891933855 created "2018-09-27" @default.
- W2891933855 creator A5028392641 @default.
- W2891933855 creator A5028489652 @default.
- W2891933855 creator A5052303723 @default.
- W2891933855 creator A5063045793 @default.
- W2891933855 creator A5072588854 @default.
- W2891933855 creator A5073619126 @default.
- W2891933855 creator A5080802164 @default.
- W2891933855 date "2018-12-01" @default.
- W2891933855 modified "2023-10-06" @default.
- W2891933855 title "High degradation and no bioavailability of artichoke miRNAs assessed using an in vitro digestion/Caco-2 cell model" @default.
- W2891933855 cites W1512778862 @default.
- W2891933855 cites W1757949072 @default.
- W2891933855 cites W1859042441 @default.
- W2891933855 cites W1900023718 @default.
- W2891933855 cites W1965082410 @default.
- W2891933855 cites W1976612131 @default.
- W2891933855 cites W1992967330 @default.
- W2891933855 cites W2009581907 @default.
- W2891933855 cites W2019034328 @default.
- W2891933855 cites W2024206172 @default.
- W2891933855 cites W2058318481 @default.
- W2891933855 cites W2063331767 @default.
- W2891933855 cites W2077423437 @default.
- W2891933855 cites W2099794145 @default.
- W2891933855 cites W2104116256 @default.
- W2891933855 cites W2113116576 @default.
- W2891933855 cites W2117072085 @default.
- W2891933855 cites W2121354606 @default.
- W2891933855 cites W2141576798 @default.
- W2891933855 cites W2149951854 @default.
- W2891933855 cites W2160446463 @default.
- W2891933855 cites W2164480958 @default.
- W2891933855 cites W2165374397 @default.
- W2891933855 cites W2166771618 @default.
- W2891933855 cites W2174104029 @default.
- W2891933855 cites W2198477092 @default.
- W2891933855 cites W2201635681 @default.
- W2891933855 cites W2233386492 @default.
- W2891933855 cites W2270037553 @default.
- W2891933855 cites W2340265793 @default.
- W2891933855 cites W2487887608 @default.
- W2891933855 cites W2511377607 @default.
- W2891933855 cites W2549746211 @default.
- W2891933855 cites W2553193414 @default.
- W2891933855 cites W2569379204 @default.
- W2891933855 cites W2600724784 @default.
- W2891933855 cites W2733152565 @default.
- W2891933855 cites W2768576987 @default.
- W2891933855 cites W2770423695 @default.
- W2891933855 cites W2789888491 @default.
- W2891933855 cites W2790551548 @default.
- W2891933855 cites W2806593469 @default.
- W2891933855 cites W2939823076 @default.
- W2891933855 cites W340278144 @default.
- W2891933855 doi "https://doi.org/10.1016/j.nutres.2018.08.007" @default.
- W2891933855 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30527261" @default.
- W2891933855 hasPublicationYear "2018" @default.
- W2891933855 type Work @default.
- W2891933855 sameAs 2891933855 @default.
- W2891933855 citedByCount "7" @default.
- W2891933855 countsByYear W28919338552019 @default.
- W2891933855 countsByYear W28919338552020 @default.
- W2891933855 countsByYear W28919338552021 @default.
- W2891933855 countsByYear W28919338552022 @default.
- W2891933855 countsByYear W28919338552023 @default.
- W2891933855 crossrefType "journal-article" @default.
- W2891933855 hasAuthorship W2891933855A5028392641 @default.
- W2891933855 hasAuthorship W2891933855A5028489652 @default.
- W2891933855 hasAuthorship W2891933855A5052303723 @default.
- W2891933855 hasAuthorship W2891933855A5063045793 @default.
- W2891933855 hasAuthorship W2891933855A5072588854 @default.
- W2891933855 hasAuthorship W2891933855A5073619126 @default.
- W2891933855 hasAuthorship W2891933855A5080802164 @default.
- W2891933855 hasConcept C104317684 @default.
- W2891933855 hasConcept C145059251 @default.
- W2891933855 hasConcept C181389837 @default.
- W2891933855 hasConcept C185592680 @default.
- W2891933855 hasConcept C193176779 @default.
- W2891933855 hasConcept C202751555 @default.
- W2891933855 hasConcept C2781357212 @default.
- W2891933855 hasConcept C31903555 @default.
- W2891933855 hasConcept C43617362 @default.
- W2891933855 hasConcept C55493867 @default.
- W2891933855 hasConcept C60644358 @default.
- W2891933855 hasConcept C86803240 @default.
- W2891933855 hasConceptScore W2891933855C104317684 @default.
- W2891933855 hasConceptScore W2891933855C145059251 @default.
- W2891933855 hasConceptScore W2891933855C181389837 @default.
- W2891933855 hasConceptScore W2891933855C185592680 @default.
- W2891933855 hasConceptScore W2891933855C193176779 @default.
- W2891933855 hasConceptScore W2891933855C202751555 @default.
- W2891933855 hasConceptScore W2891933855C2781357212 @default.
- W2891933855 hasConceptScore W2891933855C31903555 @default.
- W2891933855 hasConceptScore W2891933855C43617362 @default.
- W2891933855 hasConceptScore W2891933855C55493867 @default.
- W2891933855 hasConceptScore W2891933855C60644358 @default.